As a scientist, I have vast experience in working with unstructured data and data handling. Here, I am sharing two protocols for the analysis of neuronal life-imaging data. Including: acquisition, pre-processing, analysis and visualization algorithms.
This protocol delineates the analysis of ATP dynamics within synaptic boutons, employing the last generation of the genetically engineered ATP biosensors, called iATPSnFR2, as detailed in a recent publication
This protocol is intended to use two analysis programs: ImageJ (Fiji) and IGOR Pro (wavemetrics).
Want to know more about this protocol? click here!
This protocol delineates the analysis for the detection of spontaneous activity within synaptic boutons, employing the genetically engineered Glutamate sensors iGluSnFR, published here
This protocol is intended to use two analysis programs: ImageJ (Fiji) and IGOR Pro (wavemetrics).
Want to know more about this protocol? click here!