Monosaccharide
1 Glucose + 1 Galactose = 1 Lactose
Lactose: C12H24O11
Here are some of the mono-saccharides (think mono = one!) Glucose is the primary monosaccharide our body metabolizes, but the chemical structures of other saccharides only differ in a few atoms and can be rapidly converted to Glucose in the body.
Fructose is a monosaccharide produced in fruit, and it gets metabolized a little differently than Glucose, usually in the liver.
People with Lactose intolerance don't have enough enzymes to break this down into the monosaccharide pieces of Galactose and Glucose. Instead, tiny microbes get to eat on Lactose, which in turn produce methane gas responsible for other digestional issues.
Sucrose is the molecule that makes up granulated table sugar. Sucrose is a natural 50/50 combo of Fructose and Glucose. Hi-Fructose Corn Syrup (HFCS) is 55% Fructose/45% Glucose. Both HFCS and Sucrose are added in a lot of foods we eat. The huge amount of Fructose we consume in the Standard American Diet (SAD) has been shown to be responsible for a number of our national dietary issues.
Cellulose is polymer of Glucose that plants produce to make their cell well more sturdy and rigid. On the macro scale, this ultimately becomes the hard shelled bark that protects a tree. Humans do not produce the enzyme needed to break down Cellulose into Glucose, so it passes through our digestive system as fiber.
You are not lactose. You might be lactose-intolerant, but you aren't lactose. Lactose is the primary carbohydrate in milk.
Lactose is a disaccharide sugar composed of glucose and galactose, naturally present in the milk of mammals. Galactose is present in small amounts in some fruits and vegetables, including beets, citrus fruits, apples, brussels sprouts, carrots. legumes and nuts. Mammals have used this unique saccharide to feed there young for millions of years.
Prolactin is the hormone that stimulates the production of lactose. Prolactin is secreted in the posterior pituitary gland. This mechanism is normally triggered by nursing, and the simultaneous release of the "love hormone" oxytocin helps stimulate further production.
Lactation is sometimes stimulated in dairy cows using prolactin and oxytocin.
Lactose is produced by mammary epithelial cells in the mammary glands of mammals, including dairy cows. During lactation, when the mammary glands are activated due to pregnancy and calving, these mammary epithelial cells become active in producing milk, which includes lactose as a major carbohydrate component. The release of milk from the mammary glands is stimulated by the hormone oxytocin, which triggers the contraction of the myoepithelial cells surrounding the mammary alveoli, leading to the expulsion of milk into the ducts.
The production of milk is stimulated by the same mechanisms across the entire mammal class. The casein and whey portion of milk is made up of amino acids from the mother. Mammary epithelial cells produce or shuttle all of the other triglycerides (fats), immuno-globulines, minerals and vitamins found in milk.
Many people lose the ability to produce sufficient amounts of lactase, the enzyme needed to break down lactose. Without this enzyme, bacteria in your gut feast on the leftover lactose.
Lactobacillus and Bifidobacterium ferment the lactose to produce lactic acid. Lactic acid gives cheese its signature flavor. It's also produced by our muscles after strenuous exercise.
When microbes break down the sugar in the colon, the gas byproducts including H2, CH4 (methane) and CO2 can contribute to bloating and diarrhea.
If produced by the body or taken with food, lactase will break down the lactose into glucose and galactose, which can be absorbed in the small intestines before feeding the bacteria responsible for gas and indigestion.
Lactose is an excellent sugar for growth. As we age, the biological need for breastfeeding tapers off and natural lactase production tapers. Only 30% of adult humans are lactose tolerant and produce enough lactase for normal function. Certain genetic populations have more sensitivity to lactose, while Americans tend to have more tolerance.
Lactase supplements, like Lactaid, help consumers break down the lactose before it becomes a bacterial gut bomb. The market for these lactase supplements is expected to rise substantially in the following decades as dairy becomes more accessible to more populations.
The lac operon contains three genes: lacZ, lacY, and lacA. These genes are transcribed as a single mRNA, under control of one promoter.
The lacZ gene encodes β-galactosidase (lactase), which degrades lactose. The lacY gene product, lactose permease, transports lactose into the cell, and the lacA gene product, lactose acetylase, has an unknown and not usually necessary function.
E. coli bacteria can break down lactose due to lac operon, but it's not their favorite fuel. If glucose is around, they would much rather use that. Both the presence of lactose AND the absence of glucose are needed to fully activate the Lac operon and allow transcription.
The lac operon is a an important genetic model that is covered extensively in many biology and biochemistry courses. It was first proposed by F. Jacob and J. Monod, who were also awarded the Nobel Prize in Physiology or Medicine in 1965 for their contributions. The discovery of the operon marked a pivotal point in science - the discovery not only provided a model for gene regulation, but also established a new era in science with the emergence of molecular biology.
Cheese starts with milk, which contains both lactose and proteins like casein. When milk is curdled (using rennet or acidic substances), it separates into solid curds and liquid whey.
Bacteria are introduced in most cheese-making processes to acidify the milk and start fermentation. These bacteria consume lactose as a food source and convert it into lactic acid, which gives cheese its tangy flavor.
Fresh cheeses (such as ricotta or mozzarella) retain slightly more lactose because they undergo shorter fermentation and aging processes.
The more aged the cheese, the longer the bacteria have to consume the remaining lactose, meaning aged cheeses (like Parmesan, Cheddar, or Swiss) often have negligible lactose content, making them tolerable for most people with lactose intolerance.
Cheese likely began around 8,000-10,000 years ago, soon after humans domesticated sheep and goats. Cheese is made when rennet or an acid is added to milk.
The earliest cheeses were probably discovered by accident when milk stored in animal stomachs (which contain rennet, an enzyme) curdled.
Evidence of cheese-making has been found in ancient pots and strainers with milk-fat residues in archaeological sites from Poland to the Middle East (this one pictured is a 3000 year old pot cheese in Egypt)
Early records and depictions in tombs show that the Egyptians were fond of cheese, which they used in religious offerings and as a food source in the hot climate.
Homer mentions cheese in the Odyssey, where Polyphemus, the Cyclops, stored it. The Romans spread cheese across Europe, developing more refined methods that would become the basis for cheese varieties we recognize today.
Cheese consumption surged with the spread of refrigeration and modern dairy farming in the 20th century. Countries like France, Italy, and Greece became famous for their cheeses, and global consumption continues to rise.
PRELAB:
(5 points) List your top 5 favorite cheeses. Be objective (5 points) Create an MSDS Compliant Reagent List for your ingredients. List out the primary chemical in the ingredient (can be found via Google), and use that chemical to search for an MSDS of that item. List out general hazards.LAB:
(5 points) Create your recipe IRL. Pics or it didn't happen. Take at least 5 snapshots during the building process. Instructions would be helpful!POSTLAB:
(6 points) Complete the macronutrient breakdown sheet for your created dish. Link the spreadsheet from the copied (4 points) Mac and Cheese Postlab EvidenceBrie
Blue
Goat
Cheddar
Mozerella
Feta
Include evidence of your Thai (Milk) Tea spectroscopy lab. 1 point for evidence of completion, 2 points for evidence of pixel extraction, 2 points for completion of data table.
This doesn't have to be something complex! Use my simple recipe as an example.
(1) Include a picture of your completed Mac and Cheese product from Lab 8.
(1) List your ingredients and include your preparation instructions. Remember to include a protein, like some of these interesting choices.
(1) Name your Mac: Be Creative
(1) Rate your finished product out of 5 stars and include any adjustments you would make for your next mac
Bacon Broc Mac ⭐⭐⭐⭐
While you can't go wrong with a traditional cheddar broc, this version was a tad too soupy.
Camarones & Queso⭐⭐⭐⭐⭐
The sauce these students added to the shrimp was 👌
Collect a school milk on 11/12 or 11/13 and donate it to me for use in our MST Potluck on 11/13. Please document the collection date on the carton (only 11/12 or 11/13) and take a selfie with that date + yourself + myself. Include the picture on your site for extra credit!
Nonfat and lowfat needed for the demo. No credit for Chocolate/Strawberry milk!
Full fat (~3.25%) would be better for ice cream, but we'll make due with 1%
I don't need this stuff, only the lowfat/nonfat variety.