Research Interests

Identification and characterization of zona receptors for sperm  

The outermost layer of the mammalian egg, known as the zona, plays a critical role in the interaction between sperm and egg. However, the specific receptor(s) responsible for this interaction remain unidentified. To address this knowledge gap, our lab employs a multidisciplinary approach involving bioinformatics, protein biology, and structural biology. Through these methods, we aim to identify and characterize the elusive zona receptors. This research will significantly contribute to unraveling the intricate molecular mechanisms of mammalian fertilization.

Investigating the role of ZP2 and its genetic variations in female infertility 

Building upon our previous studies, we have established the significance of ZP2 in mediating sperm-egg interactions. Consequently, we hypothesize that certain single nucleotide polymorphisms (SNPs) in the protein may underlie cases of idiopathic female infertility. Using SNP database and various bioinformatic tools, we have identified SNPs present either in the N-term sperm binding domain of ZP2 or in the zona domain of ZP2, which may be resulting in idiopathic infertility in females. successfully identified SNPs located within the N-terminal sperm binding domain and zona domain of ZP2. Our current efforts involve population-based studies to identify novel SNPs within the Indian genome. This comprehensive investigation will shed light on the relationship between these genetic variations and idiopathic infertility in females. 

Exploring miRNAs and semen analysis in the context of male infertility 

Idiopathic male infertility represents a significant reproductive health issue. Affected individuals exhibit normal hormonal and biochemical profiles, yet display abnormalities in semen parameters. In addition to genetic, anatomical, and immunological factors, our research is investigating the association between microRNAs (miRNAs) and idiopathic male infertility. To further elucidate this connection, our lab is actively characterizing specific miRNAs and the associated pathways involved in male infertility. Furthermore, we recognize the importance of semen analysis as an excellent diagnostic and therapeutic tool for male infertility. Using simple biochemical tests, we have shown that a unique a panel of markers which can differentiate between different types of male infertility. By integrating these research avenues, we aim to contribute to a comprehensive understanding of male infertility and identify novel strategies for its diagnosis and treatment.