Investigating the complex structure and functioning of biological systems through light microscopy represents a foundational pillar of the life sciences. However, the very complexity of the samples cellular structure leads to one
of the main limitations of optical microscopy: light scattering confines it to the outer most tissue layers. In the past decades, multiphoton microscopy successfully extended the accessible depth ranges and on a different front, advances in optical wavefront shaping showed that scattering can be compensated for, even in regimes where light fully lost its initial directionality. The aim of this project is to establish scattering correction through wavefront shaping in higher- order multiphoton microscopy, namely three-photon fluorescence imaging, to surpass the scattering barrier and reach unprecedented depths in optical microscopy.
Publications:
Bernhard Rauer, Hilton B. de Aguiar, Laurent Bourdieu, Sylvain Gigan, "Scattering correcting wavefront shaping for three-photon microscopy”, under review, arXiv:2206.13191 (2022)
Bingxin Tian, Bernhard Rauer, Antoine Boniface, Jun Han, Sylvain Gigan, and Hilton B. de Aguiar, "Non-invasive chemically selective energy delivery and focusing inside a scattering medium guided by Raman scattering”, Opt. Lett. 47, 2145-2148 (2022)
Louisiane Devaud, Bernhard Rauer, Jakob Melchard, Matthias Kühmayer, Stefan Rotter, Sylvain Gigan, “Speckle Engineering through Singular Value Decomposition of the Transmission Matrix” Phys. Rev. Lett. 127, 093903 (2021)
Louisiane Devaud, Bernhard Rauer, Matthias Kühmayer, Jakob Melchard, Mickaël Mounaix, Stefan Rotter, Sylvain Gigan, “Temporal light control in complex media through the singular-value decomposition of the time-gated transmission matrix” Phys. Rev. A 105, L051501 (2022)