Marcello Montagnani, Giulia Giuffrè

Sepachrom Srl – Via Trento 33, 20017 Rho (MI) , Italy

A good practice to select the best column during a method development in liquid chromatography take in consideration several aspects. From the selection of the physical parameters of the media, as type of particle, shape, size, surface area and porosity, through the knowledge of the sample as matrix, liquid, solid or biologics, small molecules, peptides or proteins, polymers, structure and number of the components, pK and concentration, MW and solubility. A good approach to select the proper column will provide better result during the method development that challenge all chromatographers.