Carmela Maria Montone, Susy Piovesana, Sara Elsa Aita, Chiara Cavaliere, Andrea Cerrato, Aldo Laganà, Enrico Taglioni, Anna Laura Capriotti

Department of Chemistry, Università di Roma La Sapienza, Piazzale Aldo Moro 5, 00185, Rome, Italy

A new method for the online extraction and analysis of pesticides in beer was developed and validated, addressing the challenges of polar pesticides like glyphosate, AMPA, and glufosinate, which are often excluded from multi-residue methods due to their high polarity and low molecular weight. The method utilized an online trapping device with reversed-phase and anion exchange properties to retain both polar and non-polar pesticides from liquid samples. 

Chromatographic separation was optimized through a comparison of hydrophilic interaction liquid chromatography (HILIC) and reversed-phase C30, enabling effective separation of pesticides by modulating the trapping column and mobile phase composition. Validation in beer samples demonstrated recoveries between 71-112%, with relative standard deviations under 15%, and detection limits from 0.02 to 1 µg/L. The method was competitive with existing approaches and validated for 15 pesticides across a wide polarity range, providing a fast and effective solution for multi-residue analysis using RP-LC-MS/MS. Sample preparation was streamlined, requiring only degassing and dilution before online processing.

Keywords: Monolithic column, sample preparation, on-line method, click reaction

References

1. Piovesana S., Aita S. E., et al., Analytical Chemistry, 96, 11, (2024), pag. 4639–4646 DOI: 10.1021/acs.analchem.3c05706