Research
Introduction of BAE group
[노벨상 해설강연] 노벨화학상: 다가오는 유전자 교정 시대 (2020.11.02)
[1편] 이효석의 인터뷰 - 제2의 테슬라가 여기서? 유전자 가위의 모든 것 (2021.08.28)
[2편] 이효석의 인터뷰 - 크리스퍼 유전자가위 제가 다 알려드릴게요 (2021.09.04)
[브라보 K-사이언티스트] 유전자 교정 시대를 열다 - 의생명공학자 배상수 / YTN 사이언스 (2022.12.14)
[다큐S프라임 오리지널] 인류의 삶을 바꾸어 놓을 수 있는 유전자 가위 .. 불치병 치료를 넘어 생명 연장의 꿈 가능해지나.. (2023.01.02)
CRISPR-mediated RNA-guided endonucleases
In January 2013, several groups (Cho et al, Nat. Biotechnol. 2013; Cong et al, Science 2013; Mali et al, Science 2013; Hwang et al, Nat. Biotechnol. 2013; Jiang et al, Nat. Biotechnol. 2013; Jinek et al, Elife 2013) independently reported a new class of genome editing nucleases — termed RNA-guided engineered nucleases (RGENs) herein to avoid confusion with the original type II clustered regularly interspaced short palindromic repeat (CRISPR)–Cas (CRISPR-associated) adaptive immune system in bacteria — the specificity of which is mostly determined by small guide RNAs rather than by DNA-binding proteins. They cleave chromosomal DNA in a site-specific manner, which triggers endogenous DNA repair systems that result in targeted genome modification.
An RGEN is comprised of CRISPR-associated protein (Cas9), a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), which form the dualRNA–Cas9. Alternatively, an RGEN can contain Cas9 and a single-chain guide RNA (sgRNA). The guide sequence in the crRNA (fig a) or sgRNA (fig b) is complementary to a 20bp target DNA sequence known as protospacer, which is next to the 5'-NGG-3' (where N represents any nucleotide) sequence known as protospacer adjacent motif (PAM) (Kim et al, Nat. Rev. Genet. 2014).
This homepage is maintained by Molecular Genome Engineering Laboratory, Seoul National University College of Medicine, Korea.