ASISH CORE - ISH 服務介紹

RNA原位雜合 (RNA In Situ Hybridization, ISH)

植物原位雜交技術是一個功能強大的技術，可偵測及精確定位出目標基因表達在組織部位。我們已經建立了穩定的蝴蝶蘭、水稻和阿拉伯芥的組織切片技術和RNA原位雜交技術檢測目標基因在組織細胞中表達的位置。我們很高興此ISH核心服務能協助研究人員的研究。

In situ Hybridization (ISH) is a powerful technique that allows for precise localization of gene expression within a histological section. We had established stable orchid, rice, and Arabidopsis tissue sectioning technology and RNA ISH hybridization technology to detect gene expression of target gene. We are pleasure to serve and assist PIs’ researches.

Procedures of ISH experiment

Some of our ISH services had been published

References:

Ko, H.Y., Ho, L. H., Neuhaus,H. E., Guo, W. J. (2021). Transporter SlSWEET15 unloads sucrose from phloem and seed coat for fruit and seed development in tomato. Plant physiology 187 2230–2245

Hsiao, A. S., Wang, K., & Ho, T. D. (2020). An Intrinsically Disordered Protein Interacts with the Cytoskeleton for Adaptive Root Growth under Stress. Plant physiology 183(2), 570–587.

Fang, S.C., Chen, J.C., and Wei, M.J. (2016). Protocorms and Protocorm-Like Bodies Are Molecularly Distinct from Zygotic Embryonic Tissues in Phalaenopsis aphrodite. Plant Physiol 171, 2682-2700.

Jang, S. (2015). Functional Characterization of PhapLEAFY, a FLORICAULA/LEAFY Ortholog in Phalaenopsis aphrodite. Plant Cell Physiol 56, 2234-2247.

Ko, S.S., Li, M.J., Ku, M.S.-B., Ho, Y.C., Lin, Y.J., Chuang, M.H., Hsing, H.X., Lien, Y.C., Yang, H.T., Chang, H.C., and Chan, M.T. (2014). The bHLH142 transcription factor coordinates with TDR1 to modulate the expression of EAT1 and regulate pollen development in rice. Plant Cell 26, 2486-2504.

Shih, M.C., Chou, M.L., Yue, J.J., Hsu, C.T., Chang, W.J., Ko, S.S., Liao, D.C., Huang, Y.T., Chen, J.J., Yuan, J.L., Gu, X.P., and Lin, C.S. (2014). BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development. BMC Plant Biol 14, 179.