Publications

1. Joshi, A., Walimbe, A., Sarkar, S., Arora, L., Kaur, G., Jhandai, P., Chatterjee, D., Banerjee, I., & Mukhopadhyay, S. Intermolecular Energy Migration via HomoFRET Captures the Modulation in the Material Property of Phase-Separated Biomolecular Condensates (bioRxiv). https://doi.org/10.1101/2024.02.12.579899

2. Joshi, A., Avni, A., Walimbe, A., Rai, S. K., Sarkar, S., & Mukhopadhyay, S. The Hydrogen-Bonded Water Network in Phase-Separated Biomolecular Condensates (manuscript under submission).

3. Joshi, A., Walimbe, A., Avni, A., Rai, S. K., Arora, L., Sarkar, S., & Mukhopadhyay, S. Single-Molecule FRET Unmasks Structural Subpopulations and Crucial Molecular Events During FUS Low-Complexity Domain Phase Separation. Nat. Commun., 14, 7331 (2023). https://www.nature.com/articles/s41467-023-43225-y

4. Avni, A., Joshi, A., & Mukhopadhyay, S. Hydrogen-Deuterium Exchange Vibrational Raman Spectroscopy Distinguishes Distinct Amyloid Polymorphs Comprising Altered Core Architecture. J. Phys. Chem. Lett., 14, 5592–5601 (2023). https://doi.org/10.1021/acs.jpclett.3c01086

5. Mahapatra, S., Sarbahi, A., Punia, N., Joshi, A., Avni, A., Walimbe, A., & Mukhopadhyay, S. ATP Modulates Self-Perpetuating Conformational Conversion Generating Structurally Distinct Yeast Prion Amyloids that Limit Autocatalytic Amplification. J. Biol. Chem., 299, 104654 (2023). https://doi.org/10.1016/j.jbc.2023.104654

6. Gupta, A.,§ Joshi, A.,§ Arora, K., Mukhopadhyay, S., & Guptasarma, P. The Bacterial Nucleoid-Associated Proteins, HU and Dps, Condense DNA into Context-Dependent Biphasic or Multiphasic Complex Coacervates. J. Biol. Chem., 299, 104637 (2023). https://doi.org/10.1016/j.jbc.2023.104637

§Contributed equally.

7. Joshi, A., & Mukhopadhyay, S. Biophysics of Biomolecular Condensates. Biophys. J., 122, 737–740 (2023). https://doi.org/10.1016/j.bpj.2023.02.002

8. Pattanashetty, S. G., Joshi, A., Walimbe, A., & Mukhopadhyay, S. "Guidelines for Experimental Characterization of Liquid–Liquid Phase Separation in Vitro" in "Droplets of Life" 2022, Volume 1, Pages 233-249. (Publisher: Elsevier; Editor: Vladimir Uversky, University of South Florida). https://doi.org/10.1016/B978-0-12-823967-4.00012-9

9. Avni, A.,§ Joshi, A.,§ Walimbe, A., Pattanashetty, S. G., & Mukhopadhyay, S. Single-Droplet Surface-Enhanced Raman Scattering Decodes the Molecular Determinants of Liquid-Liquid Phase Separation. Nat. Commun., 13, 4378 (2022). https://doi.org/10.1038/s41467-022-32143-0

§Contributed equally.

10. Dogra, P., Roy, S. S., Joshi, A., & Mukhopadhyay, S. Hofmeister Ions Modulate the Autocatalytic Amyloidogenesis of an Intrinsically Disordered Functional Amyloid Domain via Unusual Biphasic Kinetics. J. Mol. Biol., 432, 6173–6186 (2020). https://doi.org/10.1016/j.jmb.2020.10.015

11. Dogra, P., Joshi, A., Majumdar, A., & Mukhopadhyay, S. Intermolecular Charge-Transfer Modulates Liquid-Liquid Phase Separation and Liquid-to-Solid Maturation of an Intrinsically Disordered pH-Responsive Domain. J. Am. Chem. Soc., 141, 20380–20389, (2019). https://doi.org/10.1021/jacs.9b10892

12. Rai, S. K., Khanna, R., Sarbahi, A., Shandilya, E., Joshi, A., & Mukhopadhyay S. "Chaperone-Mediated Phase Separation Regulates Liquid-to-Solid Phase Transitions of Tau into Amyloid Fibrils" (manuscript in preparation).

• Ashish Joshi, Anuja Walimbe, Anamika Avni, Sandeep K. Rai, Lisha Arora, Snehasis Sarkar, and Samrat Mukhopadhyay "Single-droplet single-molecule FRET and vibrational Raman spectroscopy unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation." Presented poster at FCSXIV, the 14th National Workshop on Fluorescence and Raman Spectroscopy meeting, organized jointly by the Indian Institute of Science Education and Research Mohali (IISER Mohali) and Institute of Nanoscience and Technology (INST) from December 9-14, 2023, at Mohali, Punjab, India.

• Ashish Joshi, Anuja Walimbe, Anamika Avni, Sandeep K. Rai, Lisha Arora, Snehasis Sarkar, and Samrat Mukhopadhyay "Single-droplet single-molecule FRET and vibrational Raman spectroscopy unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation." Presented poster at Complex Fluid International Conference (CompFlu 2023), organized by Indian Institute of Technology (IIT) Madras, Chennai, India, from December 18-20, 2023.