Annie Nixon Singleton, also known as Annie Singleton, played a significant role in the founding of Alpha Phi Alpha Fraternity, Inc., the nation's oldest intercollegiate African-American Greek-letter fraternity.

Annie Singleton, along with her husband Archie Singleton, provided a safe and supportive space in their home in Ithaca, New York, for a literary club consisting of seven Black male college students from Cornell University. The meetings held at their residence served as the genesis for the formation of Alpha Phi Alpha Fraternity in December 1906.

Annie Singleton's home on East State Street was one of several places where African American students lived while studying at Cornell University, as they were not allowed to live on campus at that time. Many African American families in Ithaca offered their homes to support these students in the racially charged environment.

In recognition of her significant contribution and support, Annie Singleton was invited as a special guest to the 1939 General Convention of Alpha Phi Alpha in New York City, where she was introduced to the brotherhood as "Mother Singleton." She became known as the "Mother of Alpha Phi Alpha."

After moving from Ithaca to Toledo, Ohio, and then to Buffalo, Annie Singleton lived with her niece until her passing in 1960. She was memorialized at the 1960 Alpha Phi Alpha Fraternity's General Convention and honored again in 2011 with a new headstone at her gravesite in Forest Lawn Cemetery.

Annie C. Singleton, or "Mother" Singleton, will be remembered for her nurturing and supportive relationship with the founding members of Alpha Phi Alpha Fraternity and her important role in its establishment.