The researchers will collect the aerial parts of Cordia dichotoma from Quirangay, Camalig, Albay, ensuring proper harvesting and careful handling. The leaves will be used for their antibacterial properties, which are linked to compounds like flavonoids, tannins, and alkaloids. After cleaning, the leaves will be air-dried for seven days, then cut into small pieces and blended. The resulting material will be weighed and placed in Erlenmeyer flasks, then mixed with 95% denatured ethanol. The mixture will be stored at 29°C for 18–24 hours.

The study will use Voluntary Response Sampling and Personal Networks, where participants are recruited through personal connections like family, friends, or colleagues. This non-probability method relies on individuals choosing to participate, typically those with an interest in the research or a personal connection to the topic.

The treatment phase will involve creating soap formulations with different concentrations of Anonang leaf extract (25%, 50%, 75%, and 100%). These formulations will be tested by the Department of Science and Technology (DOST) for antibacterial activity against E. coli. The effectiveness will be assessed based on bacterial colony count reductions and the soaps' physical stability, including color, odor, texture, viscosity, and sedimentation.

To test the antibacterial activity of Anonang leaf extract, different concentrations (100%, 75%, 50%, and 25%) will be prepared and sent to the Department of Science and Technology (DOST) for testing against E. coli. Consistent sample preparation and sterile materials will be ensured, with control tests using sterile water or ethanol to compare the extract's antibacterial effectiveness.

To make the soap, gather all ingredients, including the soap base, oils, lye, water, and Anonang extract. Prepare a clean workspace with necessary tools like mixing bowls, spatulas, and molds. Melt the soap base to the proper temperature, then mix in the Anonang extract and other additives. Pour the mixture into molds and let it set at room temperature. After curing and hardening, unmold and cut the soap into bars. Finally, package and label the bars with ingredients and usage instructions.