Nijisanji is a virtual YouTuber agency owned by Anycolor Inc. (formerly Ichikara Inc.). The agency was founded in 2018 with the purpose of promoting the use of Live2D models and streaming as opposed to the 3D and clips format popularized by Kizuna AI.

Aster Arcadia (アスター アルカディア) is a male English Virtual YouTuber affiliated with NIJISANJI EN's sixth wave "ILUNA", alongside Maria Marionette, Kyo Kaneko, Aia Amare, Scarle Yonaguni, and Ren Zotto. 

Uki Violeta (浮奇ヴィオレタ) is a male English Virtual YouTuber affiliated with NIJISANJI EN's fifth wave "Noctyx", alongside Sonny Brisko, Alban Knox, Fulgur Ovid, and Yugo Asuma. 

Selen Tatsuki (セレン 龍月) was a female English Virtual YouTuber affiliated with NIJISANJI EN's second-wave group "OBSYDIA" alongside Rosemi Lovelock and Petra Gurin.