My study clarifies the possible advantages of jaggery, a non-centrifugal sugar, in reducing seizure recovery periods in bang sensitive Drosophila melanogaster. I have made connections between different research on epilepsy treatments to emphasize how important it is to identify easily accessible and reasonably priced substitutes for the drugs that are now being used. Despite the difficulties in collecting and synchronizing flies, my methodology shows a careful approach to experimental design. Through this entire year I have learned that though unconventional and unexpected, jaggery, a non-centrifugal sugar, showed some evidence to reduce the siezure recovery times in bang sensitive Drosophila melanogaster. I was able to find some convincing evidence of my alternate hypothesis through testing using my methodolody and the principles of experimental design. I learned that even though Ayurvedic treatments are ancient methods, they still deserve to be apart of health treatment conversation today, many studies have been done on various Ayurvedic treatments relating to neurological diseases in humans and I believe these studies should be further developed and be apart of the conversation surrounding possible treatments of neurological diseases. I believe that what I learned about the content surrounding my topic this year could be expanded further as well. Some applications of this include exploring different concentrations of jaggery to determine the optimal dosages and potential variations in the effects of non-centrifugal sugar in bang sensitive Drosophila melanogaster. I also believe that conducting comparative studies with other model organisms that have more biological processes related to humans, we could also utlize human cell culture models to validate our findings in this initial study understanding broader effects of this treatment.
Deep knowledge of my research topic, methods, and outcome was demonstrated in my final presentation and oral defense. Through preparing for my final presentation specificically I was able to understand how to put my findings and content of my research paper in a different form, utilizing visuals as well as various metaphors for biological processes. I needed to make sure that individuals without a scientific background could still understand my project, an example I used to do this I used a car highway metaphor for ion channels in the brain. I showed that I could critically analyze data and I was able to articulate your results clearly. I utlized principles of statistical reasoning in order to put a meaning behind my numbers or data, this also helped individuals even those without a scientific project understand my finfings. The statistical methods also provided a ground for me to make conclusions about my initial hypotheses. For future research projects, this experience might act as a guide, highlighting the significance of careful planning and effective communication.
I believe that I could apply this to my further learning by implementing effective communication, adaptability, and continuous learning into my future endeavours. I think that learning effective communication will be important in future endeavours because it allows me to communicate my interests and educational pursuits in a clear and concise manner which will foster better understanding and engagement even by those who dont share the same interests and fascinations as me. I also think adaptability will provide a way that I could embrace change instead of fear it in my future pursuits. Adaptability can also let me develop strategies to overcome challenges I may encounter, this will ensure success in future projects. I also think in the future continous learning will provide beneficial as well, this concept will allow me to have an exploratory and curious mindset, looking for ways to learn more and make significant contributions to the scientific community and reach other educational goals I may pursue.
I proved my tenacity, flexibility, and dedication to excellence with this journey this year. I believe overcame obstacles with fortitude and gained insightful knowledge about project management and research methods. I want to apply these lessons going ahead, realizing how crucial it is to work together, be dedicated, and never stop learning in order to reach your objectives. I think that being apart of the Ap capstone program and finishing the program has provided me with a unique experience. Not only because doing research in high school is special, but also because this process has taught me lessons I will bring forward to this next chapter of my life as an undergraduate student. I have always been interested in educational pursuits specifically in the realm of science but this program has allowed me to see so many different pespectives and possibilities that students like me have pursued. Through this program, not only seeing my final outcomes was fulfilling, but seeing my peers' final outcomes were definitely very exciting and fulfilling as well, although I initially did not share the same education interests as some of my peers, seeing their interests manifest into their projects and final outcomes was very motivating and I also opened my eyes about some future interests I may want to explore. I believe in the future I will try to establish more effective communication and keep my mind open to others' pesepctives and opinions, even though I may not necessarily agree with them.
My goal is to incorporate introspection into my research methodology by consistently evaluating your advantages, disadvantages, and potential growth areas. I may improve your methodology, pinpoint successful tactics, and develop self-awareness as a researcher and a person by thinking back on my experiences. Additionally, I want to keep an eye on the bigger picture of your study and think about how my research might influence society for the better. I would like to make an effort to carry out ethical, relevant research that is in line with my objectives and values. I think that in the future I would like to conduct research projects and just pursuits and goals in general in the educational space that has a purpose for the betterment of our society.
My sincere gratitude goes out to Susanne Petri, who oversees the RCHS Biotechnology program, and Nikki Dobos and Bryan McBride, who teach AP Research, for their invaluable guidance and knowledge on lab equipment and study design. Without these members I would not have ultimately reached my final product, through this entire year these individuals have significantly helped me through each part of my process even since my intial planning stage. I would also like to thank Rebecca Uchyn for helping me with the statistical reasoning element of my project. Mrs. Uchyn has been my Ap statistics teacher this year and not only have I learned so much in her class I am also unbelievably grateful for Mrs. Uchyn's help outside class for my project, she has helped me put a meaning behind my data values that was very useful for my conclusions of my data. Mary Burnham is also an individual I would like to thank. Mrs. Burnham has provided a lot of support for me throughout my entire Capstone journey. I had Mrs. Burnham as my AP Seminar teacher and she has taught me so much surrounding writing principles and how to be a researcher in any academic setting. Moreover, Mrs. Burnham has been my Speech and Debate coach since 10th grade and I could not be more thankful of the elements of writing and debate she has taught me over the years. I can also always count on Mrs. Burnham to provide her knowledge not only on this project but on any project I may have questions about.
Abstract
In this work, bang-sensitive fruit flies (Drosophila melanogaster) are used to examine the possible effects of jaggery ingestion on seizure recovery times. This study closes a gap in the literature about the neurological effects of jaggery by investigating the impact of a natural supplement on epileptic symptoms, specifically seizure recovery times. Because jaggery reduces the generation of inflammatory cytokines, it is a non-centrifugal sugar that is rich in antioxidants and anti-inflammatory qualities. This makes it a possible treatment option for epilepsy. Fruit flies were subjected to four different concentrations of jaggery using a controlled experimental design: 0% (control), 2.5%, and 15%. A vortex mixer was used to create seizures, record them, and measure the amount of time it took for the flies to return to normal activity after their seizure. The significance of the observed variations in recovery times was evaluated using statistical analysis, notably two-sample t-tests. Findings show that there was a distinct concentration-dependent effect in the reduced seizure recovery periods that flies exposed to jaggery showed when compared to control groups. There may be a dose-response relationship because the 15% jaggery concentration group had the greatest reduction in seizure recovery periods.
These results provide evidence for the theory that jaggery or non centrifugal sugar consumption may affect the length of time it takes for bang sensitive Drosophila melanogaster to recover from seizures and may have therapeutic effects for epilepsy. Prospective investigations may examine cross-species comparisons or animal models to corroborate these results and expand on the neurological uses of jaggery. Further research into a larger spectrum of jaggery concentrations may also shed light on the fruitful use of jaggery or NCS as a possible epilepsy treatment. Given the drawbacks and side effects of the available pharmacological treatments for epilepsy, this study emphasizes the need of investigating natural supplements like jaggery as supplementary or alternative therapies. It also emphasizes the importance of tenacity and commitment in scientific research, which advances the continuous search for better epilepsy treatment approaches.
These links provide access to both my final paper and my final presentation slide show. The works cited for both the paper and presentation include all of the sources I used in creating of both of these final products. The paper corresponds to the final submission I made to College Board as well and the slides are the ones used when I recorded my final presentation for College Board.
Link to Paper: https://docs.google.com/document/d/17fUT65WG6bytFduTbWHHnbv48Sok0uim24JXgSdI8FQ/edit?usp=sharing
Link to Presentation Slide Show: https://docs.google.com/presentation/d/1U8dloqJx_eiAyFeSb8Ma9GmtAtBDiIny0wm8MzdXOSU/edit?usp=sharing
I will graduate high school this year from Rock Canyon High School. After this endeavor I am planning to continue my education journey at Creighton University in Omaha, Nebraska. I am a Dean's Scholarship recipient at Creighton and I am also apart of the direct admission dental program as well. During my time at Creighton I hope to further expand my research journey in neurological topics as I will be a neuroscience major. I am also looking to pursue both a computer science and business major as well. I am also looking to expand my experience in the medical field during my time as an undergraduate as well!