Next Generation Therapeutics

Fig. 1. Gene expression regulation by trans-acting factors targeting cis-acting elements.

The Upper panels show an mRNA 5' UTR (blue line) with functional cis-acting elements (regulatory interacting domains of the sequences) known to inhibit protein translation (green), which can be enhanced by using synthetic trans-acting factors such as dGoligos (here dG1, dG4) -designed to target these elements and minimize Gibbs energy-dependent secondary structure formation of 5'UTRs. The Lower panel shows several possible ways to regulate gene expression, including dGoligos (dG1, dG4), also known as eRNA, described for the first time in this paper. For more details see the paper.