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Abracadabra. Imagine putting a collection of tiny building blocks of various shapes and sizes into a container, shaking for a time, and opening the container to find a fully functional dollhouse, complete with doors, windows, rooms, and stairways. Joe Tien, an ENG assistant professor of biomedical engineering, proposes to use an analogous process, known as self-assembly, to organize cells into more fully functional engineered tissue than is possible with the processes currently in use.

 

 Crystal growth, one of the basic processes used by materials scientists, is one example of self-assembly. In this case chemical building blocks "grow themselves," creating complex and often useful three-dimensional geometric structures. Biological systems use self-assembly to create very complex, small-scale structures, such as cells and viruses. Tien's research, for which he recently won a Provost's Innovation Fund award, will develop biological self-assembly on a larger scale, as a process to engineer complex tissue capable of replacing damaged tissue in organs such as the liver, pancreas, blood vessels, lungs, and brain. 

 

 Current methods of tissue engineering rely on seeding mixtures of isolated cells onto a biodegradable scaffold. The addition of growth factors may promote the growth of different types of cells, but generally the resulting tissue contains a limited variety of cells, is relatively flat, and possesses little internal structure. Biologically engineered skin, for example, can be successfully grown by current methods, but functioning kidneys, hearts, and livers cannot.

 

 Tien's technique relies on creating small structures built from tiny gel components of various shapes that are coated on one or more surfaces with thin liquid films. Shaking a number of the gel components together causes them to form into three-dimensional structures -- the pieces are attracted and bound together by capillary action between the coated surfaces. The shapes of the gel components are designed so that the final structure resembles the natural structure of the tissue to be replaced.

 

 Tien's first goal is to incorporate several different kinds of cells into branching structures that will function as a vascular network. The successful creation of such networks is an essential first step toward creating complex organs that rely on a vascular system to nourish their cells and carry away waste products.

 

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