Methods

To figure out the possible transcription factors associated with the ABCC11 gene, UCSC's genome browser was utilized. Inputting ABCC11 into the browse directed me to the location of the gene within the human genome. Using the available options, I turned on tracks for ENCODE and ORegAnno data.

ENCODE is a international collaborative database which provides information on protein and RNA as well as regulatory elements that affect cells for various specific genes. ORegAnno is a resource for regulatory elements including but not limited to transcription factor binding sites, regulatory regions, RNA binding sites, etc.

The orange lines indicate transcription factor regions while the blue lines indicate regulatory regions. I specifically looked that the area just before the ABCC11 gene as it is where the promoter is located.

MACS (Model-based Analysis of ChIP-Seq) software was used to analyze available ChIP-seq data to identify transcription factor binding sites. ChIP-seq is a widely used method to determine specific transcription factor binding sites. [4]

Data from the Human Protein Atlas provided information on the varied expression levels between tissue types.

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