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We developed the Blooming Biology Tool (BBT), an assessment tool based on Bloom's Taxonomy, to assist science faculty in better aligning their assessments with their teaching activities and to help students enhance their study skills and metacognition. The work presented here shows how assessment tools, such as the BBT, can be used to guide and enhance teaching and student learning in a discipline-specific manner in postsecondary education. The BBT was first designed and extensively tested for a study in which we ranked almost 600 science questions from college life science exams and standardized tests. The BBT was then implemented in three different collegiate settings. Implementation of the BBT helped us to adjust our teaching to better enhance our students' current mastery of the material, design questions at higher cognitive skills levels, and assist students in studying for college-level exams and in writing study questions at higher levels of Bloom's Taxonomy. From this work we also created a suite of complementary tools that can assist biology faculty in creating classroom materials and exams at the appropriate level of Bloom's Taxonomy and students to successfully develop and answer questions that require higher-order cognitive skills.

Most faculty would agree that academic success should be measured not just in terms of what students can remember, but what students are able to do with their knowledge. It is commonly accepted that memorization and recall are lower-order cognitive skills (LOCS) that require only a minimum level of understanding, whereas the application of knowledge and critical thinking are higher-order cognitive skills (HOCS) that require deep conceptual understanding (Zoller, 1993). Students often have difficulty performing at these higher levels (Zoller, 1993; Bransford et al., 2000; Bailin, 2002). In the past decade, considerable effort has been directed toward developing students' critical-thinking skills by increasing student engagement in the learning process (Handelsman et al., 2004). An essential component of this reform is the development of reliable tools that reinforce and assess these new teaching strategies.

Alignment of course activities and testing strategies with learning outcomes is critical to effective course design (Wiggins and McTighe, 1998; Sundberg, 2002; Ebert-May et al., 2003; Fink, 2003; Tanner and Allen, 2004; Bissell and Lemons, 2006). Students are motivated to perform well on examinations; therefore, the cognitive challenge of exam questions can strongly influence students' study strategies (Gardiner, 1994; Scouller, 1998). If classroom activities focus on concepts requiring HOCS but faculty test only on factual recall, students quickly learn that they do not need to put forth the effort to learn the material at a high level. Similarly, if faculty primarily discuss facts and details in class but test at a higher cognitive level, students often perform poorly on examinations because they have not been given enough practice developing a deep conceptual understanding of the material. Either case of misalignment of teaching and testing leads to considerable frustration on the part of both instructor and student. Though considerable attention has been given to changing our classrooms to incorporate more active-learning strategies, not enough attention has been placed on how to better align assessment methods with learning goals. Indeed, one of the most significant ways to impact the quality of student learning is through the improvement of our assessments (Entwistle and Entwistle, 1992).

Although Bloom's lends itself to wide application, each discipline must define the original classifications within the context of their field. In biology, Bloom's has been used to design rubrics for evaluating student performance on introductory biology exams (Bissell and Lemons, 2006), develop formative assessment questions at the appropriate cognitive level (Allen and Tanner, 2002), and inform course design (Allen and Tanner, 2007). Nonetheless, there is significant need for more comprehensive assessment tools that undergraduate biology instructors can easily use to assess student learning, guide development of teaching strategies, and promote student metacognition in the biological sciences.

We have developed the Blooming Biology Tool (BBT; Table 1), which can be used to assess the Bloom's Taxonomy level of questions on biology-related topics. The BBT evolved out of a study we were asked to participate in that required us to rank more than 600 biology exam questions from a wide variety of sources including MCAT, GRE, and AP biology exams, as well as introductory biology and first-year medical school courses (Zheng et al., 2008). Here we present a detailed description of the BBT and complementary materials for use by college and university faculty and students. We also highlight how we implemented the BBT and associated learning activities in a variety of educational settings. We found the BBT a useful guide for faculty in diagnosing students' aptitudes and creating new assignments to help students develop critical-thinking skills. Our students used the BBT to create more challenging study questions and self-identify the skill levels that they find the most demanding.

The level of Bloom's that is assessed by a given type of exam question depends highly on what information is provided to the student and which inferences or connections the student must make on his or her own. It is equally important to consider the level of information previously provided through classroom instruction i.e., if students are explicitly given an answer to an analysis question in class and then given that same question on an exam, then that question only requires recall (Allen and Tanner, 2002). We would argue that labeling of diagrams, figures, etc., cannot assess higher than application-level thinking as this question-type, at most, requires students to apply their knowledge to a new situation. However, fill-in the blank, true-false, and multiple-choice questions can be designed to test analysis-level skills. It is nevertheless challenging to develop fill-in-the-blank questions that require higher than application-level thinking, but we have provided one such example (Supplemental Material A; Virology). Further, whereas multiple-choice questions can be designed to assess evaluation skills if they require students to determine relative value or merit (e.g., which data best support the following hypothesis), multiple-choice questions cannot assess synthesis-level thinking as all the answers are provided, eliminating the need for students to create new models, hypotheses, or experiments on their own. Many resources exist to assist faculty in designing high-quality, multiple-choice questions (Demetrulias et al., 1982; Udovic, 1996; Brady, 2005), and we have provided a list of some of these resources (Supplemental Material B). ff782bc1db

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