Starry night
Artwork by Jen-Hao Cheng/Sheng-hong Chen, photography by Hannah K. C. Co
Spindle-shaped human cells (RPE) were cultured in 2D surface until full confluency followed by immunofluorescent staining of YAP1 protein.
Swam intelligence revealed by self-organization of human cells into mysterious topological patterns.
Artwork by Hannah Co, Sheng-hong Chen and Lab for Cell Dynamics, photography by Hannah Co
Cells expressing fluorescent membrane marker were cultured and imaged under Zeiss Axio Observer 7 inverted microscope under controlled temperature (37 °C), atmosphere (5% CO2), and humidity (90 - 100%). Images were taken every 15 minutes using a Zeiss 20x/0.8 M27 Plan-Apochromat objective.
Microscope: Hey Gaia! How are you doing?
Gaia: I am feeling wonderful! See, those migratory tracks are leaving information for cells to self-organize into magical patterns soon~
The Gaia hypothesis by James Lovelock (1972) postulates that “the Earth is maintained in a habitable state by self-regulating feedback mechanisms involving organisms tightly coupled to their environment.” In the cellular ecosystem, cells leave behind migratory tracks as a feedback mechanism to their neighbors. Cells gather this feedback from other cells to change their space exploration
Artwork by Liya
Expression of Blue fluorescent protein in human fibroblasts.
I wanted to see the expression location of the protein, so I attached a fluorescent marker to it. Although the results didn't meet my expectations, I unexpectedly found joy in the process...
Photography by Yae-Huei Liou
ZEISS LSM980 and cat Mimi
Both items are lovely and cost a lot...
Pin-Yi Song
bacterial culture on LB agar contaminated with mold, Marker
An optimistic researcher could always make himself happy even when accident happened.
Experiment and photography by Hanna K.C. Co, analysis and artwork by Jen-Hao Cheng
Human cells grown and self-organize for two days. Angles (colored based on -90 to 90 degrees) of cell orientation and alignment quantified using Q-tensor calculation on phase contrast image. Lines quantified by entropy.
Derivation of colors from cells see: https://photos.app.goo.gl/M6Zizn9cDKMLVgrWA
Vibrant colors captures the large heterogeneity of spindle-shaped cells upon naturally occurring self-organization, vividly placing spotlight on the diversity and differences of individuals. Everyone is different. Still, each individual collectively aligns with their neighbors, celebrating individual differences as well as organization of community.
Won under Looking at Life
Creator
Experiment and Imaging by Ming-Hui Lin
Materials and Methods
The DAPI signal is labeled by mpl-magma. MAP-2-staining (Cyan Hot) indicate the neuron morphology.
Description
The aggregation of primary rat cortical neurons.
Won under Still LIfe
Creator
Meng-Jia Wang
Materials and Methods
iphone 11
Description
After the rain, the small puddles reflect the serene and beautiful scenery of the IMB.
Won under Life and Beyond
Creator
Experiment by Hannah Co, processing and digital sketch by Jen-Hao Cheng
Materials and Methods
Immunofluorescence of a transcription factor overlaid with DAPI, done on self-organized spindle-shaped cells, touched up by digital sketch
Description
In glowing cells, a sparrow born,
To soar beyond the culture's morn,
A flight of light, a fleeting show,
From lab to sky, it's free to go.
-description generated by ChatGPT
Won under Life in Motion (tied)
Creator
Experiment and imaging by Poulomi
Materials and Methods
The active neurons were "Trapped" in the Fos-CreER mouse brain by tamoxifen drug injection to express tdTomato fluorescent protein.
Description
Amidst the vast expanse of darkness, the soma of a neuron extends its delicate processes, reaching out like tendrils of curiosity. It seeks to find kindred partners, yearning to intertwine and form intricate bonds in a symphony of neural connection.