The Aria II Cell sorter combines the sensitivity of a digital bench-top analyzer with the functionality of a high precision sorter. Improvements in fludics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with less stress on the sorted cells and enhanced overall sensitivity. Each Aria is configured slightly differently. 

Rate:

$107.25/hr.

$91.50/hr. (after hours)

External User rate:

$141.25/hr.

The Cytek Aurora is equipped with five lasers, 355nm, 405nm, 488nm, 561nm and 638nm and is capable of up to 67 detection channles (64 fluorecent, FCS, blue and violet laser SSC). An optical design with high sensitivity Coarse Wavelength Division Mulitplexing 8-16 channel semiconductor detector array for each laser provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations with dyes emitting in the 365-830nm range without reconfiguring the system for each application. The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. Flat-Top beam profiles, combined with a uniquely designed fluidic system, translate to outstanding performance at high sample flow rates. The spectral unmixing results in the reduction cellular autoflourescents enabling more sensitive detection of dim signals. .

Rate:

$45.25/hr.

External User rate:

$75.00/hr.

3 Laser - 8 Color Analyzer with 488nm, 647nm and 405nm lasers 

Rate:

$41.50/hr.

External User Rate:

$71.00/hr.

The Canto RUO is equipped with 4 lasers; 488nm, 561nm, 647nm, 405nm capable of 13 color detection. 

Rate:

$41.50/hr.

External User Rate:

$71.00/hr.

The Symphony A3 is equipped with 5 lasers; 488nm blue laser, 561 nm yellow/green laser, 637nm red laser, 405nm violet laser, and 350nm UV laser. Capable of simultaneous detection of 24 fluorochromes and 26 parameter data acquisition. The HTS plate reader accommodates 96-well plates for a more automated high-speed acquisition of samples. 

If you publish data generated in the Cell Analysis Facility, please remember to acknowledge the services provided and the award for the Symphony A3. This helps us meet our obligation to report to NIH successful usage of the instrumentation award. Acknowledgments also aids in requesting funding for future instrumentation!

Suggested acknowledgment for the Symphony A3:

“Flow cytometry data was acquired through the University of Washington, Cell Analysis Facility Shared Resource Lab, with NIH award 1S10OD024979-01A1 funding for the Symphony A3 ”

Rate:

$45.25/hr.

External User Rate:

$75.00/hr.

The Fortessa is equipped with 5 lasers; 488nm blue laser, 532nm green laser, 628nm red laser, 405nm violet laser, and 355nm UV laser. Capable of simultaneous detection of 18 fluorochromes and 20 parameter data acquisition. The HTS plate reader accommodates 96-well plates for more automated high speed acquisition of samples. 

Rate:

$45.25/hr.

External User Rate:

$75.00/hr.

Amnis Imagestream X Mark II is an imaging cytometer.

Correlated image and flow cytometry data gives this instrument unique capabilities not available on other platforms.

Equipped with 488nm, 405nm and 647nm lasers, six high resolution channel, 20X 40X and 60X magnification

Rate:

$115.75/hr

External User Rate:

$150.50/hr.