MMDL publications

MMDL publications about our assays

• • Seilie AM, Chang M, Hanron AE, Billman ZP, Stone BC, Zhou K, Olsen TM, Daza G, Ortega J, Cruz K, Smith NT, Healy SA, Neal J, Wallis CK, Fritzen E, Shelton L, VonGoedert T, Wong-Madden S, Kappe SHI, Fishbaugher M, Betz W, Hume JCC, Talley AK, Wald A, Plowe CV, Adams M, Fahle GA, Cowan E, Duffy PE, Kublin JG, Murphy SC. 2019. Beyond blood smears for malaria - clinical validation of the Plasmodium 18S rRNA as a biomarker for controlled human malaria infections. Amer J Trop Med Hyg 100(6):1466-1476. (https://doi.org/10.4269/ajtmh.19-0094)

    • This manuscript describes the FDA Biomarker Qualification.  

  • Murphy SC, Prentice JL, Williamson K, Wallis CK, Fang FC, Fried M, Pinzon C, Wang R, Talley AK, Kappe SHI, Duffy PE, Cookson BT. 2012. Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials Am J Trop Med Hyg. 86(3):383–94.

  • Murphy SC, Hermsen CC, Douglas AD, Edwards NJ, Petersen P, Fahle GA, Adams M, Berry AA, Billman ZP, Gilbert SC, Laurens MC, Leroy O, Lyke KE, Plowe CV, Seilie AM, Strauss KA, Teelen K, Hill AVS, Sauerwein RW. 2014. External quality assurance of malaria nucleic acid testing for clinical trials and eradication surveillance PLoS ONE9(5): e97398.

  • Murphy SC, Daza G, Chang M, Coombs RW. 2012. Laser cutting eliminates nucleic acid cross-contamination in dried blood spot processing. J Clin Microbiol. 50(12):4128-30.

Publications involving MMDL testing services

• • Murphy SC, Shott JP, Parikh S, Etter P, Prescott WR, Stewart VA. 2013. Malaria Diagnostics in Clinical Trials. Am J Trop Med Hyg. 89(5):824-839.

  • Hodgson SH, Douglas AD, Edwards NJ, Kimani D, Elias SC, Chang M, Daza G, Seilie AM, Magiri C, Muia A, Juma EA, Cole AO, Rampling TW, Anagnostou NA, Gilbert SC, Hoffman SL, Draper SJ, Bejon P, Ogutu B, Marsh K, Hill AVS, Murphy SC. 2015. Increased sample volume and use of quantitative reverse-transcription PCR can improve prediction of liver-to-blood inoculum size in controlled human malaria infection studies. Malaria J 14:33. (doi: 10.1186/s12936-015-0541-6) (PMC4318195)

  • Hanron AH, Billman ZP, Seilie AM, Chang M, Murphy SC. 2016. Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR. Diag Microbiol Infect Dis . doi: 10.1016/j.diagmicrobio.2016.11.021.

  • Hanron AE, Billman ZP, Seilie AM, Olsen TM, Fishbaugher M, Chang M, Rueckle T, Andenmatten N, Greenhouse B, Arinaitwe E, Rek J, Das S, Domingo GJ, Shipman K, Kappe SH, Kublin JG, Murphy SC. 2017. Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs. Malar J 16(1):208. doi: 10.1186/s12936-017-1863-3.

  • Das S, Jang IK, Barney B, Peck R, Rek J, Arinaitwe E, Adama H, Murphy M, Imwong M, Ling C, Proux S, Haohankhunnatham W, Rist M, Seilie AM, Hanron A, Daza G, Chang M, Nakamura T, Kalnoky M, Labarre P, Murphy SC, McCarthy J, Nosten F, Greenhouse B, Domingo GJ. 2017. Performance of a high-sensitivity rapid diagnostic test for Plasmodium falciparum malaria in asymptomatic individuals from Uganda and Myanmar and naïve human challenge infections. Am J Trop Med Hyg 97(5):1540-1550 (PMID 28820709) (https://doi.org/10.4269/ajtmh.17-0245)

  • Fahle GA, Luethy PM, Murphy SC, Seilie AM, Xie YL, Lau C, Tisdale JF, Hsieh MM, Reinhardt JL, Lau AF. 2018. Case Report: Diagnostic challenges of prolonged post-treatment clearance of Plasmodium nucleic acids in a pre-transplant autosplenectomized patient with sickle cell disease. Malaria J. 17:23 (https://doi.org/10.1186/s12936-017-2152-x)

  • Murphy SC, Kas A, Stone BC, Bevan MJ. 2013. A cytotoxic T cell response to a liver-stage Plasmodium antigen is not boosted by repeated sporozoite immunizations. Proc Natl Acad Sci USA 110(15): 6055–6060.

  • Douglass AN, Kain HS, Abdullahi M, Arang N, Austin LS, Mikolajczak SA, Billman ZP, Hume JC, Murphy SC, Kappe SH, Kaushansky A. 2015. Host-based prophylaxis successfully targets liver stage malaria parasites. Mol Ther. 23(5):857-65. (https://doi: 10.1038/mt.2015.18) (PMC4427874)

  • Sack BK, Mikolajczak SA, Fishbaugher M, Vaughan AM, Flannery EL, Nyugen T, Betz W, Navarro MJ, Foquet L, Steel RWJ, Billman ZP, Murphy SC, Hoffman SL, Chakravarty S, Sim BKL, Behet M, Reuling IJ, Walk J, Scholzen A, Sauerwein RW, Ishizuka AS, Flynn B, Seder RA, Kappe SHI. 2017. Humoral protection against mosquito bite-transmitted Plasmodium falciparum infection in humanized mice. NPJ Vaccines 2 (article 27). https://doi:10.1038/s41541-017-0028-2.

  • Flannery EL, Foquet L, Chuenchob V, Fishbaugher M, Billman ZP, Foquet L, Navarro MJ, Betz W, Olsen TO, Lee J, Scafer C, Sack B, Wilson E, Bial J, Campo B, Charman S, Saunders J, Murphy SC, Phillips MA,  Kappe SHI, Mikolajczak S. 2017. Assessment of drug efficacy against P. falciparum liver stages in a liver-chimeric humanized mouse model. JCI Insight (https://doi.org/10.1172/jci.insight.92587)

  • Kisalu N, Idris A, Weidle C, Flores-Garcia Y, Flynn B, Sack B, Murphy SC, Kappe S, Schön A, Freire E, Francica J, Miller A, Riera S, Kanekiyo M, Wheatley A, McDermott A, Farney SK, Chuang G, Zhang B, Natasha KC, Chakravarty S, Kwong P, Bhatia S, Sim BKL, Hoffman S, Zavala F, Pancera M, Seder R. 2018. A human monoclonal antibody prevents malaria infection and defines a site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature Med 24(4):408-416. (https://doi.org/10.1038/nm.4512)

  • Foquet L, Schafer C, Minkah NK, Alanine DGW, Flannery EL, Steel RWJ, Camargo N, Fishbaugher M, Betz W, Nguyen T, Billman ZP, Wilson EM, Bial J, Murphy SC, Draper SJ, Mikolajczak SA, Kappe SHI. 2018. Plasmodium falciparum liver stage infection and transition to stable blood stage infection in liver-humanized and blood-humanized FRGN KO mice. Frontiers Immunol 9:524 (https://doi.org/10.3389/fimmu.2018.00524)

  • Murphy SC, Ishizuka AS, Billman ZP, Olsen TM, Seilie AM, Chang M, Smith N, Chuenchob V, Chakravarty S, Sim BKL, Kappe SHI, Hoffman SL, Seder RA. 2018. Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood. Malaria J 17:275. (https://doi.org/10.1186/s12936-018-2422-2)

  • Jang IK, Tyler A, Lyman C, Kahn M, Kalnoky M, Rek J, Arinaitwe E, Adrama H, Murphy M, Imwong M, Ling C, Proux S, Haohankhunnatham W, Rist M, Seilie A, Hanron A, Daza G, Chang M, Das S, Barney R, Rashid A, Landier J, Boyle D, Murphy S, McCarthy J, Nosten F, Greenhouse B, Domingo G. 2018. Simultaneous Quantification of Plasmodium Antigens and Host Factor CRP in Asymptomatic Individuals with Confirmed Malaria Using a Novel Multiplex Immunoassay. J Clin Microbiol JCM.00948-18 (https://doi.org/10.1128/JCM.00948-18)

  • Healy SA, Murphy SC, Hume JCC, Shelton L, Kuntz S, Van Voorhis WC, Moodie Z, Metch B, Wang R, Silver-Brace T, Fishbaugher M, Kennedy M, Finney OC, Chaturvedi R, Hobbs CV, Warner-Lubin M, Talley AK, Wong-Madden S, Stuart K, Wald A, Kappe SH, Kublin JG, Duffy PE. 2019. Chemoprophylaxis vaccination: Phase 1 study to explore stage-specific immunity to Plasmodium falciparum in U.S. adults. Clin Infect Dis ciz1010. (http://doi.org/10.1093/cid/ciz1010). PMID: 31621832.

  • Cunningham JA, Thomson RM, Murphy SC, de la Paz Ade M, Ding XC, Incardona S, Legrand E, Lucchi NW, Menard D, Nsobya SL, Saez AC, Chiodini PL, Shrivastava J. 2020. WHO malaria nucleic acid amplification test external quality assessment scheme: results of distribution programmes one to three. Malaria J. 19(1):129. (https://doi.org/10.1186/s12936-020-03200-0). PMID: 32228615.

  • Wockner LF, Hoffmann I, Webb L, Mordmüller B, Murphy SC, Kublin JG, O'Rourke P, McCarthy JS, Marquart L. 2020. Growth Rate of Plasmodium falciparum: Analysis of Parasite Growth Data From Malaria Volunteer Infection Studies. J Infect Dis 221(6):963-972. (https://doi.org/10.1093/infdis/jiz557). PMID: 31679015. ​

  • Jimmy OT, Emmanuel O, Gonzaga A, Basil O, Jimmy L, Murphy SC, Egwang TG. 2020. Using the ultrasensitive Alere Pf Malaria Ag HRP-2TM rapid diagnostic test in the field and clinic in northeastern Uganda. Am J Trop Med Hyg. 103(2):778-784. (https://doi.org/10.4269/ajtmh.19-0653). PMID: 32602431

  • Goswami D, Betz W, Kamal N, Parthiban C, Brager C, Schäfer C, Camargo N, Nguyen T, Murphy SC, Vaughan A, Kappe S. 2020. A replication-competent late liver stage-attenuated human malaria parasite. JCI Insight. (https://doi.org/10.1172/jci.insight.135589). PMID: 32484795.

  • Schäfer S, Roobsoong W, Kangwanrangsan N, Bardelli M, Rawlinson TA, Dambrauskas N, Trakhimets O, Parthiban C, Goswami D, Reynolds LM, Kennedy SY, Flannery EL, Murphy SC, Sather DN, Draper SJ, Sattabongkot J, Mikolajczak SA, Kappe SHI. 2020. A humanized mouse model for Plasmodium vivax to test interventions that block liver stage to blood stage transition and blood stage infection. iScience. 23(8):101381. (https://doi.org/10.1016/j.isci.2020.101381). PMID: 32739836

  • Vasquez AM, Velez GJ, Medina AC, Serra-Casas E, Campillo A, Gonzalez IJ, Murphy SC, Seilie AM, Tobon-Castano A. 2020. Evaluation of highly sensitive diagnostic tools for the detection of P. falciparum in pregnant women attending antenatal care visits in Colombia. BMC Preg Childbirth. 20(1):440. (https://doi.org/10.1186/s12884-020-03114-4). PMID: 32736543

  • Shears MJ, Seilie AM, Sim BKL, Hoffman SL, Murphy SC. 2020. Quantification of Plasmodium knowlesi versus Plasmodium falciparum in the rhesus liver: implications for malaria vaccine studies in rhesus models. Malar J. 19(1):313. (https://doi.org/10.1186/s12936-020-03385-4). PMID: 32867784.

  • Kublin J, Murphy SC, Maenza J, Seilie AM, Jain JP, Goswami B, Berger D, Spera D, Stein DS, Zhao R, Hackling M, Soon RL, Czartoski JL, Potochnic MA, Duke E, Chang M, Vaughan A, Kappe SHI, Leong FJ, Pertel P. Prince WT. 2021. Safety, pharmacokinetics and causal prophylactic efficacy of KAF156 in a Plasmodium falciparum human infection study. Clin Infect Dis. ciaa952. (https://doi.org/10.1093/cid/ciaa952). PMID: 32644127.

  • Murphy SC, Deye GA, Sim BKL, Galbiati S, Kennedy JK, Cohen KW, Chakravarty S, KC N, Abebe Y, James ER, Kublin JG, Hoffman SL, Richie TL, Jackson LA. 2021. PfSPZ-CVac efficacy against malaria increases from 0% to 75% when administered in the absence of erythrocyte stage parasitemia: a randomized, placebo-controlled trial with controlled human malaria infection. PLoS Pathog. 17(5): e1009594. (https://doi.org/10.1371/journal.ppat.1009594). PMID: 34048504.

  • Mwakingwe-Omari A, Healy SA, Lane J, Cook DM, Kalhori S, Wyatt C, Kolluri A, Marte-Salcedo O, Imeru A, Nason M, Ding LK, Decederfelt H, Duan J, Neal J, Raiten J, Lee G, Hume JCC< Jeon JE, Ikpeama I, KC N, Chakravarty S, Murshedkar T, Church LWP, Manoj A, Gunasekera A, Anderson C, Murphy SC, March S, Bhatia SN, James ER, Billingsley PF, Sim BKL, Richie TL, Zaidi I, Hoffman SL, Duffy PE. 2021. Two chemoattenuated PfSPZ malaria vaccines induce sterile hepatic immunity. Nature. 595(7866):289-294. (https://doi.org/10.1038/s41586-021-03684-z). PMID: 34194041.

  • Chang M, Johnson S, Seilie AM, Hergott D, Murphy SC. 2021. Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies. Malar J. https://doi.org/10.1186/s12936-021-03907-8). PMID: 34620192.

  • Shaffer D, Kumwenda J, Chen H, Akelo V, Angira F, Kosgei J, Tonui R, Ssali F, McKhann A, Hogg E, Stewart VA, Murphy SC, Coombs R, Schooley R; A5297 Team. 2022. Brief report: no differences between lopinavir/ritonavir and non-nucleoside reverse transcriptase inhibitor-based antiretroviral therapy on clearance of Plasmodium falciparum subclinical parasitemia in adults living with HIV starting treatment (A5297). J Acquir Immune Defic Syndr. 89(2):178-182. (https://doi.org/10.1097/QAI.0000000000002839). PMID: 34693933.

  • Watson FN, Shears MJ, Matsubara J, Kalata AC, Seilie AM, Talavera IC, Olsen TM, Tsuji M, Chakravarty S, Sim BKL, Hoffman SL, Murphy SC. 2022. Cryopreserved sporozoites with and without the glycolipid adjuvant 7DW8-5 protect in prime-and-trap malaria vaccination. Am J Trop Med Hyg. 106(4):1227-1236 (https://doi.org/10.4269/ajtmh.21-1084). PMID: 35226868.

  • Matsubara J, Chang M, Seilie AM, Murphy SC. 2022. Flow cytometric sorting of infected erythrocytes demonstrates reliable detection of individual ring-stage Plasmodium falciparum parasites by Plasmodium 18S rRNA reverse transcription PCR. Am J Trop Med Hyg. 11:tpmd211226. (https://doi.org/10.4269/ajtmh.21-1226). PMID: 35405648.

  • Goswami D, Kumar S, Betz W, Armstrong JA, Haile MT, Camargo N, Parthiban C. Seilie AM, Murphy SC, Vaughan AM, Kappe S. 2022. A Plasmodium falciparum ATP binding cassette transporter is essential for liver stage entry into schizogony. iScience 8;25(5):104224. (https://doi.org/10.1016/j.isci.2022.104224): PMID: 35521513

  • Wilder BK, Vigdorovich V, Carbonetti S, Minkah N, Hertoghs N, Raappana A, Cardamone H, Oliver BG, Trakhimets O, Kumar S, Dambrauskas N, Arredondo SA, Camargo N, Murphy SC, Kappe SHI, Sather DN. 2022. Anti-TRAP/SSP2 monoclonal antibodies can inhibit sporozoite infection and enhance protection of anti-CSP monoclonal antibodies. NPJ Vaccines. 7(1):58. (https://doi.org/10.1038/s41541-022-00480-2). PMID: 35618791

  • Hergott DEB, Owalla TJ, Balkus JE, Apio B, Lema J, Cemeri B, Akileng A, Seilie AM, Chavtur C, Staubus W, Chang M, Egwang TG, Murphy SC. 2022. Feasibility of community at-home dried blood spot collection combined with pooled reverse transcription PCR as a viable and convenient method for malaria epidemiology studies. Malar J 221. (https://doi.org/10.1186/s12936-022-04239-x). PMID: 35836179. PMC9284728.

  • Kimingi HW, Kinyua AW, Achieng NA, Wambui KM, Mwangi S, Nguti R, Kivisi CA, Jensen ATR, Bejon P, Kapulu MC, Abdi AI, Kinyanjui SM; CHMI-SIKA Study Team (including Murphy SC). 2022. Breadth of antibodies to Plasmodium falciparum variant surface antigens is associated with immunity in a controlled human malaria infection study. Front Immunol. 13:894770. (https://doi.org/10.3389/fimmu.2022.894770). PMID: 35711446.

  • Chakravarty S, Shears MJ, James ER, Rai U, KC N, Murphy SC, Hoffman SL. 2022. Efficient infection of non-human primates with purified, cryopreserved Plasmodium knowlesi sporozoites. Malaria J. 21(1):247. (https://doi.org/10.1186/s12936-022-04261-z). PMID: 36030292.

  • Flannery EL, Kangwangrangsan N, Chuenchob V, Roobsong W, Fishbugher M, Zhou K, Billman ZP, Martinson T, Olsen TM, Schafer C, Campo B, Murphy SC, Mikolajczak SA, Kappe SHI. Sattabongkot J. 2022. Plasmodium vivax latent liver infection is characterized by persistent hypnozoites, hypnozoite-derived schizonts, and time-dependent efficacy of primaquine. Molec Ther 26:427-40. (https://doi.org/10.1016/j.omtm.2022.07.016). PMID: 36092359.

  • Murphy SC, Vaughan AM, Kublin JG, Fishbauger M, Seilie AM, Cruz KP, Mankowski T, Firat M, Magee S, Betz W, Kain H, Camargo N, Haile MT, Gibson J, Fritzen E, Hertoghs N, Sather DN, Deye GA, Galbiati S, Geber C, Butts J, Jackson LA, Kappe SHI 2022. A genetically-engineered Plasmodium falciparum parasite vaccine provides protection from controlled human malaria infection. Sci Transl Med. 14(659):eabn9709. (https://doi.org/10.1126/scitranslmed.abn9709). PMID: 36001680.

  • Owalla TJ, Hergott D, Seilie AM, Staubus W, Chavtur C, Chang M, Kublin JG, Egwang TG, Murphy SC. 2022. Rethinking detection of pre-existing and intervening Plasmodium infections in malaria clinical trials. Front Immunol. (https://doi.org/10.3389/fimmu.2022.1003452). PMID 36203582.   

  • Franke-Fayard B, Marin-Mogollon C, Geurten F, Chevalley-Maurel S, Ramesar J, Kroeze H, Baalbergen EE, Wessels E, Soulard V, Martinson T, Aleshnick M, Huijs A, Subudhi A, Miyazaki Y, Othman A, Kolli S, Lamers O, Roques M, Stanway R, Murphy S, Foquet L, Mendes A, Prudencio M, Dechering K, Heussler V, Pain A, Wilder B, Roestenberg M, Janse C. 2022. Generation and preclinical evaluation of a genetically attenuated malaria parasite, GA2, that arrests growth late in the liver. npj Vaccines. 7(1):139. doi: 10.1038/s41541-022-00558-x. PMID: 36333336.

  • Odera DO, Tuju J, Mwai K, Nkumama IN, Fürle K, Chege T, Kimathi R, Diehl S, Musasia FK, Rosenkranz M, Njuguna P, Hamaluba M, Kapulu MC, Frank R; CHMI-SIKA Study Team; Osier FHA, Abdi AI, Chi PC, de Laurent Z, Jao I, Kamuya D, Kamuyu G, Makale J, Murungi L, Musyoki J, Muthui M, Mwacharo J, Kariuki S, Mwanga D, Mwongeli J, Ndungu F, Njue M, Nyangweso G, Kimani D, Ngoi JM, Musembi J, Ngoto O, Otieno E, Ooko M, Shangala J, Wambua J, Mohammed KS, Omuoyo D, Mosobo M, Kibinge N, Kinyanjui S, Bejon P, Lowe B, Marsh K, Marsh V, Abebe Y, Billingsley PF, Sim BKL, Hoffman SL, James ER, Richie TL, Audi A, Olewe F, Oloo J, Ongecha J, Ongas MO, Koskei N, Bull PC, Hodgson SH, Kivisi C, Imwong M, Murphy SC, Ogutu B, Tarning J, Winterberg M, Williams TN. 2023. Anti-merozoite antibodies induce natural killer cell effector function and are associated with immunity against malaria. Sci Transl Med. 15(682):eabn5993. doi: 10.1126/scitranslmed.abn5993. PMID: 36753561.

  • Chavtur C*, Staubus WJ*, Ho M, Hergott DEB, Seilie AM, Healy S, Duffy P, Jackson L, Talley A, Kappe SHI, Hoffman SL, Richie TL, Kublin JG, Chang M, Murphy SC. 2023. Plasmodium 18S rRNA biomarker clearance after FDA-approved antimalarial treatment in controlled human malaria infection trials. Open Forum Infect Dis (*co-first authors) (submitted 11/13/2022, accepted 4/10/2023)

  • Global Malaria Programme. 2015. A WHO external quality assurance scheme for malaria nucleic acid amplification testing (WHO meeting report). 8–9 June 2015, London, United Kingdom. (http://www.who.int/malaria/publications/atoz/9789241509985_eng.pdf) (Contributor)

  • Chi P, Awuor Owino E, Jao I, Olewe F, Ogutu B, Bejon P, Kapulu M, Kamuya D, Marsh V, CHMI-SIKA Study Team. 2021. Understanding the benefits and burdens associated with a malaria human infection study in Kenya: experiences of study volunteers and other stakeholders. 2021. Trials. 22(1):494. doi: 10.1186/s13063-021-05455-7. (https://doi.org/10.1186/s13063-021-05455-7). PMID: 34311781.

  • Kapulu MC, Njuguna P, Hamaluba M, Kimani D, Ngoi JM, Musembi J, Ngoto O, Otieno E, Billingsley PF, Controlled Human Malaria Infection in Semi-Immune Kenyan Adults (CHMI-SIKA) Study Team. 2021. Safety and PCR monitoring in 161 semi-immune Kenyan adults following controlled human malaria infection. JCI Insight. 6(17):146443. (https://doi.org/10.1172/jci.insight.146443). PMID: 34264864.

  • Kimingi HW, Kinyua AW, Achieng NA, Wambui KM, Mwangi S, Nguti R, Kivisi CA, Jensen ATR, Bejon P, Kapulu MC, Abdi AI, Kinyanjui SM; CHMI-SIKA Study Team. 2022. Breadth of Antibodies to Plasmodium falciparum Variant Surface Antigens Is Associated With Immunity in a Controlled Human Malaria Infection Study. Front Immunol. 13:894770. doi: 10.3389/fimmu.2022.894770. PMID: 35711446; PMCID: PMC9195513.

  • Kariuki SN, Macharia AW, Makale J, Nyamu W, Hoffman SL, Kapulu MC, Bejon P, Rayner JC, Williams TN; for the CHMI-SIKA Study Team. 2023. The Dantu blood group prevents parasite growth in vivo: Evidence from a controlled human malaria infection study. Elife. 12:e83874. doi: 10.7554/eLife.83874. PMID: 37310872; PMCID: PMC10264070.