Biofilm are microorganisms entrapped in a 3D extracellular matrix (ECM) of their own making; the ECM comprises extracellular polysaccharides, DNA, and proteins, the latter mostly in the form of amyloid fibrils. This project analyses the mechanism of amyloid formation in both Gram-positive and Gram-negative bacteria, introduces two different synthetic peptide design approaches to inhibit amyloid formation, and translates the results to clinically relevant models.