The proteins used for this study were found during a literature review about the presence of Quinolizidine Alkaloids (QAs) in the Genisteae Tribe.
The uniprot database (The UniProt Consortium 2019) was searched for each protein and a BLAST sequence was run for each.
The resulting fasta files were then run with the Clustal Omega tool (Madeira 2019) to compare the proteins and visualize them with the use of Jalview (Waterhouse 2009). This allows for an in-depth analysis of the similarity of the protein sequences.
Finally, phylogenetic trees of these sequences were then created with Simple Phylogeny, which will allow us to gauge evolutionary distance between the proteins.