publications

Hydrogel-tissue chemistry (HTC) steps toward tissue functionalization. The main steps for transformation of the tissue, as shown for (a) the diagrammed cell prior to the HTC process, include (b) hydrogel-monomer infusion followed by cross-linking to native macromolecules and then gelation, (c) stringent delipidation with detergent without the risk of extensive biomolecule loss or structural disruption, (d) optical clearing, refractive index matching, high-resolution volumetric imaging (through delipidized tissue), and read-out of biomolecules tethered to the tissue-hydrogel coordinate system, shown in green.

Hydrogel-Tissue Chemistry

Mano T, Albanese A, Dodt H-U, Erturk A, Gradinaru V, Treweek JB, Miyawaki A, Chung K, Ueda HR. (2018) Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy. J. Neurosci., 38, 9330–9337.
Gradinaru V, Treweek JB, Overton K, and Deisseroth K. (2018) Hydrogel-Tissue Chemistry: Principles and Applications. Annu. Rev. Biophys., 47, 355-376.
Treweek JB and Gradinaru V. (2016) Extracting structural and functional features of widely distributed biological circuits with single cell resolution via tissue clearing and delivery vectors. Curr. Opin. Biotechnol., 40, 193-207. PMCID: PMC4975678
Treweek JB, Chan KY, Flytzanis NC, Yang B, Deverman BE, Greenbaum A, Lignell A, Xiao C, Cai L, Ladinsky MS, Bjorkman PJ, Fowlkes CC, and Gradinaru V. (2015) Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping. Nat. Protoc., 10, 1860-1896. PMCID: PMC4917295
Yang B, Treweek, JB, Kulkarni RP, Deverman BE, Chen CK, Lubeck E, Shah S, Cai L, and Gradinaru V. (2014) Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell, 158, 945-958. PMCID: PMC4153367

Systems Neuroscience

Cho JR, Treweek JB, Robinson JE, Xiao C, Bremner LR, Greenbaum A, and Gradinaru V. (2017) Dorsal Raphe Dopamine Neurons Modulate Arousal and Promote Wakefulness by Salient Stimuli. Neuron, 94, 1205-1219.e8.
Xiao C, Cho JR, Zhou C, Treweek JB, Chan K, McKinney SL, Yang B, and Gradinaru V. (2016) Cholinergic Mesopontine Signals Govern Locomotion and Reward through Dissociable Midbrain Pathways. Neuron, 90, 333-347. PMCID: PMC4840478
Treweek JB, Jaferi A, Colago EE, Zhou P, and Pickel VM. (2009) Electron microscopic localization of corticotropin-releasing factor (CRF) and CRF receptor in rat and mouse central nucleus of the amygdala. J. Comp. Neurol., 512, 323-335. PMCID: PMC2873768

Biomedical Sciences

Cohen A, Treweek JB, Edwards S, Leão RM, Koob GF, and George O. (2015) Extended access to nicotine leads to a CRF1 receptor dependent increase in anxiety-like behavior and hyperalgesia in rats. Addict. Biol., 20, 56-68. PMCID: PMC3859819
Treweek JB, Dickerson TJ, and Janda KD. (2009) Drugs of abuse that mediate advanced glycation end product formation: a chemical link to disease pathology. Acc. Chem. Res., 42, 659-669. PMCID: PMC2982794
Treweek JB, Moreno AY, and Janda KD. (2009) SLEEPLESS-ness and insomnia in fruit flies. Angew. Chem. Int. Ed. Engl., 48, 438-440.

Immunotherapy

Treweek JB and Janda KD. (2012) An antidote for acute cocaine toxicity. Mol. Pharm., 9, 969-978. PMCID: PMC3319285
Janda KD and Treweek JB. (2011) Vaccines targeting drugs of abuse: is the glass half-empty or half full? Nat. Rev. Immunol., 12, 67-72.
Treweek JB, Roberts AJ, and Janda KD. (2011) Immunopharmacotherapeutic manifolds and modulation of cocaine overdose. Pharmacol. Biochem. Behav., 98, 474-484. PMCID: PMC3069656
Treweek JB, Roberts AJ, and Janda KD. (2010) Superadditive Effects of Ethanol and Flunitrazepam: Implications of Using Immunopharmacotherapy as a Therapeutic. Mol. Pharm., 7, 2056-2068. PMCID: PMC2997924
Treweek JB, Sun C, Mayorov AV, Qi L, Levy CL, Roberts AJ, Dickerson TJ, and Janda KD. (2008) Prevention of drug-induced memory impairment by immunopharmacotherapy. J. Med. Chem., 51, 6866-6875. PMCID: PMC2661766
Treweek JB, Wee S, Koob GF, Dickerson TJ, and Janda KD. (2007) Self-vaccination by methamphetamine glycation products chemically links chronic drug abuse and cardiovascular disease. Proc. Natl. Acad. Sci. USA, 104, 11580-11584. PMCID: PMC1913859

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