Achieved Results

Short Scientific Report of the Project

The execution of project was carried out according to the implementation plan of the project NanoAnSys, PN-III-P1-1.1-TE-2019-1276, TE80/01.09.2020, thus fulfilling all scientific objectives and successfully achieving the performance indicators corresponding to this stage. The aim of the project NanoAnSys was to obtain a system of nano (nm) dimensions, anthocyanin carrier (AnSys), fluorescent and sensitive to pH variation intracellular. Among the objectives set, the following have been achieved: (i) the design and development of the anthocyanin transport nanosystem; (ii) functionalization and physico-chemical characterization of the nanosystem; (iii) efficient encapsulation and release of anthocyanins from the nanosystem; (iv) evaluation of the cytotoxicity of free anthocyanins and the nanosystem on melanoma cells; (v) intracellular monitoring of AnSys (internalisation, localisation, controlled release). The performance indicators achieved based on the data obtained and reported for stage 2 are: 2 Articles (ISI indexed, with IF = 4.072 and 4.411), 8 Conferences (2 oral presentations / 6 posters), 1 Master's Thesis, 1 PhD Thesis.

Performance indicators:

Articles indexed ISI with IF:

1. Nistor, M.; Focsan, M.; Gaina, L.; Cenariu, M.; Pintea, A.; Socaciu, C.; Rugina, D., Real-time fluorescence imaging of anthocyanins complexed with diphenylboric acid 2-aminoethyl inside B16-F10 melanoma cells. Phytochemistry. 2021, 189, 112849, IF=4.072.

2. Nistor, M.; Pop, R.; Daescu, A.; Pintea, A.; Socaciu, C.; Rugina, D., Anthocyanins as Key Phytochemicals Acting for the Prevention of Metabolic Diseases: An Overview. Molecules. 2022, 27(13), IF=4.411.

3. Dăescu A., Ispas C., Nistor M., Pop R., Tabaran F., Diaconeasa Z., Matea C., Rugina D., Pintea A. Microcapsules for a local delivery of anthocyanins, under preparation, will be submitted in 2 weeks to Applied Sciences, 2022, IF=2.838.

4. Nistor, M.; Pop, R.; Daescu, A.; Focsan, M.; Pintea, A.; Rugina, D., Polymeric pH-responsive nanocapsules loaded with anthocyanins for melanoma intracellular delivery, release and therapy, under preparation.

Conferences:

1. Nistor, M.; Focsan, M.; Pop, R.; Daescu, A.; Pintea, A.; Rugina, D., Nanoparticles designed for anthocyanins transport inside melanoma cells. 20^th International Conference Life Sciences for Sustainable Development, 23-24 September 2021, Cluj-Napoca, Romania.

2. Daescu, A.; Nistor, M.; Rugina, D.; Pintea, A., Influence of pH on stability of anthocyanins pigments from Amelanchier lamarkii juneberries. 20^th International Conference Life Sciences for Sustainable Development, 23-24 September 2021, Cluj-Napoca, Romania.

3. Nistor, M.; Daescu, A.; Popan, R.; Boldianu, A.; Focsan, M.; Pintea, A.; Rugina, D., Fluorescent pH-sensitive nanosystems for anthocyanins delivery and tracking inside melanoma cells. 35^th Conference of the European Colloid & Interface Society, 5-10 September 2021, Athens, Greece.

4. Boldianu A., Nanosistem fluorescent pH-sensibil pentru monitorizarea internalizării antocianilor în celule de melanom, Simpozion Științific Studențesc, 21 Iulie 2021, USAMV Cluj-Napoca, Cluj-Napoca, Romania.

5. Nistor, M.; Pop, R.; Daescu, A.; Focsan, M.; Pintea, A.; Rugina, D., Polymeric pH-responsive nanocapsules loaded with anthocyanins for melanoma intracellular delivery, release and therapy. 25^th International Union of Biochemistry and Molecular Biology – 46^th Federation of European Biochemical Societies – 15^th Pan-American Association for Biochemistry and Molecular Biology International Congress, 9-14 Iuilie 2022, Lisbon, Portugal.

6. Madalina Nistor, Roxana Pop, Monica Focsan, Septimiu Tripon, Lucian Barbu, Adela Daescu, Adrian Boldianu, Adela Pintea, Dumitrita Rugina, pH-responsive nanocapsules with in vitro therapeutic action: anthocyanins encapsulation and controlled release inside b16-f10 melanoma cells, 21^th International Conference Life Sciences for Sustainable Development, 15-17 September 2022, Cluj-Napoca, Romania.

7. Daescu A., Ispas C., Nistor M., Pop R., Tabaran F., Diaconeasa Z., Matea C., Rugina D., Pintea A. Microcapsules for a local delivery targeting the diabetic retina, 21^th International Conference Life Sciences for Sustainable Development, 15-17 September 2022, Cluj-Napoca, Romania.

8. Daescu A., Nistor M., Rugina D., Nicolescu A., Pintea A. Stability, bioaccessibility and enzyme inhibition potential of anthocyanins pigments from Amelanchier lamarkii berries, 10^th International Symposium on Recent Advances in Food Analysis, 6-8 September 2022, Praga, Republica Ceha.

Master Thesis:

Pop Roxana, Nanocapsule sensibile la pH pentru livrarea, localizarea intracelulară și eliberarea antocianilor în celule de melanom (pH-sensitive nanocapsules for delivery, intracellular localization and release of anthocyanins in melanoma cells), coordonatori: Banciu Manuela si Rugina Dumitrita, Iulie 2021, UBB Cluj-Napoca, Romania.

PhD Thesis:

Nistor Mădălina-Lorena, Anthocyanins as phytochemicals with biomedical applications: biochemical characterisation and in vitro biological action, coordinator: Socaciu Carmen, Iulie 2022, USAMV Cluj-Napoca, Romania.