The World Health Organization warns that the incidence of skin cancers has been increased over the past decades. This warning forced the scientists to develop new strategies for melanoma therapy. In this context, our goal is to obtain pH-sensitive nanosystems to carry anthocyanins (AnSys) directly into melanoma cells, where their delivery, subcellular localization and their pH-controlled release will be monitored. For this purpose the main objectives are: (i) to design and develop the smart fluorescent pH-sensitive nanosystem as carrier for anthocyanins; (ii) to evaluate the efficient release of the entrapped anthocyanins under specific pH conditions, by liquid cromatography coupled with mass spectrometry; (iii) to monitor the pH-dependent intracellular processes after the AnSys internalization into melanoma cells, by using non-invasive high-performance fluorescence microscopy techniques. Therefore, the current proposal is highly scored in novelty-seeking, because there is no data available on a similar pH responsive nanocarrier system, able to facilitate the controlled release of anthocyanins in melanoma tumor cells.