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Spheroids represent one of the most prevalent and widely utilized examples of scaffold-free 3D cell culture systems. These three-dimensional cellular aggregates are formed by self-assembly and cohesion of cells in the absence of an external scaffold or matrix.
PROTOCOL OVERVIEW
Published protocols on spheroid generation can be applied to both primary cells and established cell lines.
Publications:
Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening (2018). Griner, L. M., Gampa, K., Do, T., Nguyen, H., Farley, D., Hogan, C. J., Auld, D. S., Silver, S. J. J. Vis. Exp.(139), e57476, doi:10.3791/57476.
Patient-derived tumor spheroid cultures as a promising tool to assist personalized therapeutic decisions in breast cancer (2022). Hofmann, S., Cohen-Harazi, R., Maizels, Y., & Koman, I. Translational cancer research, 11(1), 134–147. https://doi.org/10.21037/tcr-21-1577
EXPECTED OUTPUT
Representative photos of MCF-7 cells grown in to spheroids at different seeding densities using ultralow attachment plate. Phase contrast photos were taken 3 days after seeding under an inverted microscope with 4x magnification. Photo c/o Allen Joy Corachea, Science Research Specialist, Regen Lab.
APPLICATIONS
Publication: Griner, L. M., Gampa, K., Do, T., Nguyen, H., Farley, D., Hogan, C. J., Auld, D. S., Silver, S. J. Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening.J. Vis. Exp.(139), e57476, doi:10.3791/57476 (2018).
Publication: Berens, E. B., Holy, J. M., Riegel, A. T., Wellstein, A. A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting.J. Vis. Exp.(105), e53409, doi:10.3791/53409 (2015).
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