Genome sequencing

The Instrument:

The 3500xL Genetic Analyzer is a 24-capillary sequencing platform suitable for a wide variety of applications, including de novo sequencing, resequencing (mutational profiling), micro satellite analysis, and SNP validation or screening. Most applications can be run on a single polymer and capillary array. Designed to detect up to six fluorescent dyes simultaneously, the 3500 Series enables high levels of multiplexing for fragment analysis applications, increasing throughput and data points per run, which can help lower the cost per sample.

Make: Applied Biosystems™  

Model: 3500xL Genetic Analyzer

Feature and specifications:

• Amperage:15 A

• Run Time: 30 to 140 min. (varies depending on run module)

• Number of Capillaries: 24 Capillaries

• Capillary Length: 36 cm, 50 cm

• Green Features: Energy efficient, Fewer resources used and less waste

• Throughput: Up to 1104 samples per day (using run module Short Read Seq POP7)

• Voltage: Electrophoresis Voltage - Up to 20 kV

• Operating Environment: Temperature: 15°C-30°C, Humidity: 20–80% (non-condensing)

Applications:

·         Clinical Research: Genetic testing, pharmacogenomics, inherited disease research.

·         Forensic Science: DNA Profiling, paternity testing, disaster victim identification.

·         Genetic Research: Sequencing, SNP genotyping, mutation detection.

·         Pharmaceutical Development: Drug development, biomarker discovery.

·         Microbial Genomics: Pathogen identification, antibiotic resistance studies.

·         Environmental Biology: Biodiversity Studies, analyzes genetic divers.

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