• Discovery of a new selective Endoplasmic Reticulum Aminopeptidase 1 inhibitor for Hedgehog-dependent cancers treatment 

Bufalieri F*, Cucinotta A*, Cammarone S*, Agnoli F, Basili I, Ferri G, Quaglio D, Caimano M, Capriotti AL, Montone CM, Severini LL, Tempora P, D'Amico S, Juretich F, Semrau MS, Navacci S, Conenna M, Bordone R, Spallotta F, Garavaglia S, Storici P, Ghirga F, Laganà A, Locatelli F, Ayrault O, Infante P, Botta B, Mori M, Fruci D, Di Marcotullio L. 

Molecular Therapy 2025. doi:10.1016/j.ymthe.2025.10.036. PMID: 41108078.

• MEX3A is a diagnostic, independent prognostic biomarker and a promising therapeutic target in glioblastoma 

Bufalieri F*, Armocida D*, Cucinotta A, Familiari P, Di Magno L, Serraino A, Adabbo G, Agnoli F, Lospinoso Severini L, Antonelli M, Frati A, Canettieri G, Infante P, Santoro A, D'Angelo L, Di Marcotullio L.

Frontiers in Oncology 2025.  doi: 10.3389/fonc.2025.1585592. PMID: 40958871

• SALL4 is a CRL3^REN/KCTD11 substrate that drives Sonic Hedgehog-dependent medulloblastoma 

Lospinoso Severini L*, Loricchio E*, Navacci S*, Basili I, Alfonsi R, Bernardi F, Moretti M, Conenna M, Cucinotta A, Coni S, Petroni M, De Smaele E, Giannini G, Maroder M, Canettieri G, Mastronuzzi A, Guardavaccaro D, Ayrault O, Infante P, Bufalieri F, Di Marcotullio L.

Cell Death & Differentiation 2024. doi: 10.1038/s41418-023-01246-6. PMID: 38062245

• ERAP1 as an emerging therapeutic target for medulloblastoma 

Bufalieri F, Fruci D, Di Marcotullio L.

Trends in Cancer 2022.  doi: 10.1016/j.trecan.2021.09.005. PMID: 34686465.

• Glabrescione B delivery by self-assembling micelles efficiently inhibits tumor growth in preclinical models of Hedgehog-dependent medulloblastoma 

Infante P*, Malfanti A*, Quaglio D*, Balducci S, De Martin S, Bufalieri F, Mastrotto F, Basili I, Garofalo M, Lospinoso Severini L, Mori M, Manni I, Moretti M, Nicoletti C, Piaggio G, Caliceti P, Botta B, Ghirga F, Salmaso S, Di Marcotullio L. 

Cancer Letters 2021. doi: 10.1016/j.canlet.2020.11.028. PMID: 33249196.

• The SHH/GLI signaling pathway: a therapeutic target for medulloblastoma 

Lospinoso Severini L*, Ghirga F*, Bufalieri F, Quaglio D, Infante P, Di Marcotullio L. 

Expert Opinion on Therapeutic Targets 2020. doi: 10.1080/14728222.2020.1823967. PMID: 32990091.

• The RNA-Binding Ubiquitin Ligase MEX3A Affects Glioblastoma Tumorigenesis by Inducing Ubiquitylation and Degradation of RIG-I 

Bufalieri F*, Caimano M*, Lospinoso Severini L, Basili I, Paglia F, Sampirisi L, Loricchio E, Petroni M, Canettieri G, Santoro A, D'Angelo L, Infante P, Di Marcotullio L.

Cancers (Basel) 2020. doi: 10.3390/cancers12020321. PMID: 32019099.

• ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP 

Bufalieri F*, Infante P*, Bernardi F, Caimano M, Romania P, Moretti M, Lospinoso Severini L, Talbot J, Melaiu O, Tanori M, Di Magno L, Bellavia D, Capalbo C, Puget S, De Smaele E, Canettieri G, Guardavaccaro D, Busino L, Peschiaroli A, Pazzaglia S, Giannini G, Melino G, Locatelli F, Gulino A, Ayrault O, Fruci D, Di Marcotullio L. 

Nature Communications 2019.  doi: 10.1038/s41467-019-11093-0. PMID: 31341163.

• Itch/β-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis 

Infante P*, Faedda R*, Bernardi F*, Bufalieri F, Lospinoso Severini L, Alfonsi R, Mazzà D, Siler M, Coni S, Po A, Petroni M, Ferretti E, Mori M, De Smaele E, Canettieri G, Capalbo C, Maroder M, Screpanti I, Kool M, Pfister SM, Guardavaccaro D, Gulino A, Di Marcotullio L. 

Nature Communications 2018. doi: 10.1038/s41467-018-03339-0. PMID: 29515120.

• Gli1/DNA interaction is a druggable target for Hedgehog-dependent tumors.  

Infante P,* Mori M*, Alfonsi R*, Ghirga F, Aiello F, Toscano S, Ingallina C, Siler M, Cucchi D, Po A, Miele E, D'Amico D, Canettieri G, De Smaele E, Ferretti E, Screpanti I, Uccello Barretta G, Botta M, Botta B, Gulino A, Di Marcotullio L. 

EMBO Journal 2015. doi: 10.15252/embj.201489213. PMID: 25476449.

• Histone deacetylase and Cullin3-REN(KCTD11) ubiquitin ligase interplay regulates Hedgehog signalling through Gli acetylation.  

Canettieri G*, Di Marcotullio L*, Greco A, Coni S, Antonucci L, Infante P, Pietrosanti L, De Smaele E, Ferretti E, Miele E, Pelloni M, De Simone G, Pedone EM, Gallinari P, Giorgi A, Steinkühler C, Vitagliano L, Pedone C, Schinin ME, Screpanti I, Gulino A. 

Nature Cell Biology 2010. doi: 10.1038/ncb2013. PMID: 20081843.

• Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination.  

Di Marcotullio L*, Ferretti E*, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. 

Nature Cell Biology 2006. doi: 10.1038/ncb1510. PMID: 17115028.

• REN(KCTD11) is a suppressor of Hedgehog signaling and is deleted in human medulloblastoma.  

Di Marcotullio L*, Ferretti E*, De Smaele E*, Argenti B, Mincione C, Zazzeroni F, Gallo R, Masuelli L, Napolitano M, Maroder M, Modesti A, Giangaspero F, Screpanti I, Alesse E, Gulino A.

PNAS 2004. doi: 10.1073/pnas.0400690101. PMID: 15249678.