Nour, S. et al. A review of accelerated wound healing approaches: biomaterial- assisted tissue remodeling. Journal of Materials Science: Materials in Medicine 30, (2019).
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Accelerated Wound Healing Research
Accelerated Wound Healing Research
Wound healing is currently a great area of interest for therapeutics and biomaterials research. The Air Force Office of Scientific Research (AFOSR) has funded our project to investigate the benefits of cellular reprogramming in wound healing. However, current in vitro and in vivo skin wounding models are not faithful to the conditions and anatomy of actual human skin. Wounding is often done manually, which can be difficult to reproduce.
In Vitro 3D Wound Healing Model
In Vitro 3D Wound Healing Model
My lab members and I developed a multiweek experiment with four stages: dermal construction, epidermal construction, automated wounding, and cellular reprogramming. Over the course of one summer, I was able to complete stages one and two, with plans for stages three and four in place. Our in vitro model mimics the construction and composition of actual skin. Automated wounding is much more reproducible than manual wounding.
Human skin compared to biofabricated in vitro skin equivalent
IMG_1132.mov
The biofabricated skin equivalent constructs were automatically wounded with the BioAssemblyBot 400 and biopsy punches.
Experiments
Experiments
Collagen hydrogel and fibroblast construct after 1 day
Collagen hydrogel and fibroblast construct after 10 days
Agarose test gel with automated biopsy punch wound
H&E stained full thickness skin equivalent after 22 days (20x)
H&E stained full thickness skin equivalent after 22 days (40x)
BJ-Incucyte-CellCycle.mp4Human dermal fibroblast progressing through the cell cycle
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