(* - contributed equally, # - corresponding author, Kersten lab member)
39. Ousley, D.A.*, Wang, X.*, Mydy, L.S., Kersten, R.D.# Plant genome mining of burpitides from fused precursor peptides. Methods Enzymol., in press. (2025) DOI: https://doi.org/10.1016/bs.mie.2025.04.007
38. Wang, X.*, Shafiq, K.*, Ousley, D.A.*, Chigumba, D.N., Davis, D., McDonough, K.M., Mydy, L.S., Sexton, J.Z., Kersten, R.D.# Large-scale transcriptome mining enables macrocyclic diversification and improved bioactivity of the stephanotic acid scaffold. Nat. Commun. 16, 1-18 (2025). https://www.nature.com/articles/s41467-025-59428-4
37. Damiani, T., Jarmusch, A.K., Aron, A.T., Petras, D., Phelan, V.V., Zhao, H.N., Bittremieux, W., Acharya, D.D., Ahmed, M., Bauermeister, A., Bertin, M.J., Boudreau, P.D., Borges, R.M., Bowen, B.P., Brown, C.J., Chagas, F.O., Clevenger, K.D., Correia, M.S.P., Crandall, W.J., Crüsemann, M., Fahy, E., Fiehn, O., Garg, N., Gerwick, W.H., Gilbert, J.R., Globisch, D., Gomes, P.W.P., Heuckeroth, S., James, C.A., Jarmusch, S.A., Kakhkhorov, S.A., Kang, K.B., Kessler, N., Kersten, R.D., Kim, H., Kirk, R.D., Kohlbacher, O., Kontou, E.E., Liu, K., Lizama-Chamu, I., Luu, G.T., Luzzatto Knaan, T., Mannochio-Russo, H., Marty, M.T., Matsuzawa, Y., McAvoy, A.C., McCall, L.-I., Mohamed, O.G., Nahor, O., Neuweger, H., Niedermeyer, T.H.J., Nishida, K., Northen, T.R., Overdahl, K.E., Rainer, J., Reher, R., Rodriguez, E., Sachsenberg, T.T., Sanchez, L.M., Schmid, R., Stevens, C., Subramaniam, S., Tian, Z., Tripathi, A., Tsugawa, H., van der Hooft, J.J.J., Vicini, A., Walter, A., Weber, T., Xiong, Q., Xu, T., Pluskal, T., Dorrestein, P.C., Wang, M. A universal language for finding mass spectrometry data patterns. Nat. Methods 22, 1247-1254 (2025). https://www.nature.com/articles/s41592-025-02660-z
36. Yan, D., Zhou, M., Adduri, A., Zhuang, Y., Guler, M., Liu, S., Shin, H., Kovach, T., Oh, G., Liu, X., Deng, Y., Wang, X., Cao, L., Sherman, D.H., Schultz, P.J., Kersten, R.D., Clement, J.A., Tripathi, A.#, Behsaz, B.#, Mohimani, H.#. Discovering type I cis-AT polyketides through computational mass spectrometry and genome mining with Seq2PKS. Nat. Commun. 15, 5356 (2024). https://www.nature.com/articles/s41467-024-49587-1
35. Gomes, P.W.P., Mannochio-Russo, H., Schmid, R., Zuffa, S., Damiani, T., Quiros-Guerrero, L.-M., Caraballo-Rodríguez, A. M., Nina Zhao, H.N., Yang, H., Xing, S., Charron-Lamoureux, V., Chigumba, D.N., Sedio, B.E., Myers, J.A., Allard, P.-M., Harwood, T.V., Tamayo-Castillo, G., Kang, K.B., Defossez, E., Koolen, H.H.F., da Silva, M.N., e Silva, C.Y.Y., Rasmann, S., Walker, T.W.N., Glauser, G., Chaves-Fallas, J.M., David, B., Kim, H., Lee, K.H., Kim, M.J., Choi, W.J., Keum, Y.-S., de Lima, E.J.S.P., de Medeiros, L.S., Bataglion, G.A., Costa, E.V., da Silva, F.M.A., Carvalho, A.R.V., Reis, J.D.E., Pamplona, S., Jeong, E., Lee, K., Kim, G.J., Kil, Y.-S., Nam, J.-W., Choi, H., Han, Y.K., Park, S.Y., Lee, K.Y., Hu, C., Dong, Y., Sang, S., Morrison, C.R., Borges, R.M., Teixeira, A.M., Lee, S.Y., Lee, B.S., Jeong, S.Y., Kim, K.H., Rutz, A., Gaudry, A., Bruelhart, E., Kappers, I.F., Karlova, R., Meisenburg, M., Berdaguer, R., Tello, J.S., Henderson, D., Cayola, L., Wright, S.J., Allen, D.N., Anderson-Teixeira, K.J., Baltzer, J.L., Lutz, J.A., McMahon, S.M., Parker, G.G., Parker, J.D., Northen, T.R., Bowen, B.P., Pluskal, T., van der Hooft, J.J.J., Carver, J.J., Bandeira, N., Pullman, B.S., Wolfender, J.-L., Kersten, R.D., Wang, M., Dorrestein, P.C. plantMASST-Community-driven chemotaxonomic digitization of plants. bioRxiv, pp.2024-05 (2024). https://www.biorxiv.org/content/10.1101/2024.05.13.593988v1
34. Chekan, J.R.#, Mydy, L.S., Pasquale, M.A., Kersten, R.D.#, 2024. Plant peptides–redefining an area of ribosomally synthesized and post-translationally modified peptides. Nat. Prod. Rep. 41, 1020-1059 (2024). https://pubs.rsc.org/en/content/articlelanding/2024/np/d3np00042g
33. Mydy, L.S.#, Hungerford, J., Chigumba, D.N., Konwerski, J.R., Jantzi, S.C., Wang, D., Smith, J.L., Kersten, R.D.#. An intramolecular macrocyclase in plant ribosomal peptide biosynthesis. Nat. Chem. Biol. 20, 530-540 (2024). https://www.nature.com/articles/s41589-024-01552-1
32. Lee, Y.Y., Guler, M., Chigumba, D.N., Wang, S., Mittal, N., Miller, C., Krummenacher, B., Liu, H., Cao, L., Kannan, A., Narayan, K., Slocum, S.T., Roth, B.L., Gurevich, A., Behsaz, B., Kersten, R.D., Mohimani, H.# HypoRiPPAtlas as an Atlas of hypothetical natural products for mass spectrometry database search. Nat. Commun. 14, 4219 (2023). https://www.nature.com/articles/s41467-023-39905-4
31. Yao, S.#, Sapkota, D., Hungerford, J.A., Kersten, R.D. Jujube Fruit Metabolomic Profiles Reveal Cultivar Differences and Function as Cultivar Fingerprints. Plants 12, 2313 (2023). https://www.mdpi.com/2223-7747/12/12/2313
30. Smith, D.J., Kharbush, J.J., Kersten, R.D., Dick, G.J.# Uptake of phytoplankton-derived carbon and cobalamins by novel acidobacteria genera in microcystis blooms inferred from metagenomic and metatranscriptomic evidence. AEM 88, e01803-21 (2022). https://journals.asm.org/doi/10.1128/aem.01803-21
29. Kersten, R.D.#, Mydy, L.S., Fallon, T.R., de Waal, F., Shafiq, K., Wotring, J.W., Sexton, J.Z., Weng, J.K.# Gene-Guided Discovery and Ribosomal Biosynthesis of Moroidin Peptides. J. Am. Chem. Soc. 144, 7686-7692 (2022). https://pubs.acs.org/doi/10.1021/jacs.2c00014
28. Chigumba, D.N.*, Mydy, L.S.*, de Waal, F.*, Li, W., Shafiq, K., Wotring, J.W., Mohamed, O.G., Mladenovic, T., Tripathi, A., Sexton, J.Z., Kautsar, S. Medema, M.H.#, Kersten, R.D.# Discovery and biosynthesis of cyclic plant peptides via autocatalytic cyclases. Nat. Chem. Biol. 18, 18-28 (2022). https://www.nature.com/articles/s41589-021-00892-6
27. Mydy, L.S., Chigumba, D.N., Kersten, R.D.# 2021. Plant Copper Metalloenzymes As Prospects for New Metabolism Involving Aromatic Compounds. Front. Plant Sci. 12, 692108-692108 (2021). https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2021.692108/full
Pre-Michigan
26. Kersten, R.D., Weng, J.-K. Gene-guided discovery and engineering of branched cyclic peptides in plants. Proc. Natl. Acad. Sci. U. S. A. 115, E10961-E10969 (2018). https://www.pnas.org/doi/10.1073/pnas.1813993115
25. Wada, N., Kersten, R., Iwai, T., Lee, S., Sakurai, F., Kikuchi, T., Fujita, D., Fujita, M., Weng, J.-K. Crystalline Sponge‐based Structural Analysis of Crude Natural Product Extracts. Angew. Chem. Int. Ed. Engl. 57, 3671-3675 (2018). https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201713219
24. Kersten, R.D.*, Lee, S.*, Fujita, D. Pluskal, T., Kram, S., Smith, J.E., Iwai, T., Noel, J.P., Fujita, M., Weng, J.-K. A Red Algal Bourbonane Sesquiterpene Synthase Defined by Microgram-scale NMR-coupled Crystalline Sponge XRD Analysis. J. Am. Chem. Soc. 139, 16838-16844 (2017, * - contributed equally to this work) https://pubs.acs.org/doi/10.1021/jacs.7b09452
23. Wang, M., Carver, J.J., Phelan, V.V., Sanchez, L.M. (and 123 others, including Kersten. R.D.), Sharing and community curation of mass spectrometry data with GNPS. Nat. Biotechnol. 34, 828, (2016). https://www.nature.com/articles/nbt.3597
22. Kersten, R.D., Diedrich, J.K., Yates III, J.R., Noel, J.P. Mechanism-based post-translational modification and inactivation in terpene synthases. ACS Chem. Biol. 10, 2501-2511 (2015). https://pubs.acs.org/doi/10.1021/acschembio.5b00539
21. Michelsen, C.F., Watrous, J., Glaring, M.A., Kersten, R., Koyama, N., Dorrestein, P.C., Stougaard, P. Nonribosomal Peptides, Key Biocontrol Components for Pseudomonas fluorescens In5, Isolated from a Greenlandic Suppressive Soil. mBio, 6, e00079-15 (2015). https://journals.asm.org/doi/10.1128/mbio.00079-15
20. Mohimani, H., Liu, W. T., Kersten, R. D., Moore, B. S., Dorrestein, P. C., Pevzner, P. A. NRPquest: Coupling Mass Spectrometry and Genome Mining for Nonribosomal Peptide Discovery. J. Nat. Prod. 77, 1902–1909 (2014). https://pubs.acs.org/doi/10.1021/np500370c
19. Agarwal, V., El Gamal, A.A., Yamanaka, K., Poth, D., Kersten, R.D., Schorn, M., Allen, E.E. Moore, B.S., Biosynthesis of polybrominated organic compounds by marine bacteria. Nat. Chem. Biol. 10, 640-647 (2014). https://www.nature.com/articles/nchembio.1564
18. Mohimani, H., Kersten, R.D., Liu, W.-T., Wang, M., Purvine, S.O., Wu, S., Brewer, H.M., Pasa-Tolic, L., Moore, B.S., Pevzner, P.A., Dorrestein, P.C. Automated genome mining of ribosomal peptide natural products. ACS Chem. Biol. 9, 1545–1551 (2014). https://pubs.acs.org/doi/10.1021/cb500199h
17. Yamanaka, K., Reynolds, K.A., Kersten, R.D., Ryan, K.S., Gonzalez, D.J., Nizet, V., Dorrestein, P.C., Moore, B.S. Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc. Natl. Acad. Sci. U. S. A. 111, 1957-1962 (2014). https://www.pnas.org/doi/10.1073/pnas.1319584111
16. Liu, W.T., Lamsa, A., Wong, W.R., Boudreau, P.D., Kersten, R., Peng, Y., Moree, W.J., Duggan, B.M., Moore, B.S., Gerwick, W.H., Linington, R.G., Pogliano, K., Dorrestein, P.C. MS/MS-based networking and peptidogenomics guided genome mining revealed the stenothricin gene cluster in Streptomyces roseosporus. J. Antibiot. (Tokyo) 67, 99-104 (2014). https://www.nature.com/articles/ja201399
15. Kersten, R.D., Ziemert, N., Gonzalez, D.J., Duggan, B.M., Nizet, V., Dorrestein, P.C., Moore, B.S. Glycogenomics as a mass spectrometry-guided genome-mining method for microbial glycosylated molecules. Proc. Natl. Acad. Sci. U. S. A. 110, E4407-4416 (2013). https://www.pnas.org/doi/10.1073/pnas.1315492110
14. Kersten, R.D., Lane, A.L., Nett, M., Richter, T.K., Duggan, B.M., Dorrestein, P.C., Moore, B.S. Bioactivity-Guided Genome Mining Reveals the Lomaiviticin Biosynthetic Gene Cluster in Salinispora tropica. Chembiochem 14, 955-962 (2013). https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.201300147
13. Nguyen, D.D., Wu, C.-H., Moree, W.J., Lamsa, A., Medema, M.H., Zhao, X., Gavilan, R.G., Aparicio, M., Atencio, L., Jackson, C., Ballesteros, J., Sanchez, J., Watrous, J.D., Phelan, V.V., van de Wiel, C., Kersten, R.D., Mehnaz, S., De Mot, R., Shank, E.A., Charusanti, P., Nagarajan, H., Duggan, B.M., Moore, B.S., Bandeira, N., Palsson, B., Pogliano, K., Gutiérrez, M., Dorrestein P.C. MS/MS networking guided analysis of molecule and gene cluster families. Proc. Natl. Acad. Sci. U. S. A. 110, E2611-2620 (2013). https://www.pnas.org/doi/abs/10.1073/pnas.1303471110
12. Ross, A.C., Xu, Y., Lu, L., Kersten, R.D., Shao, Z., Al-Suwailem, A.M., Dorrestein, P.C., Qian, P.Y., Moore, B.S. Biosynthetic Multitasking Facilitates Thalassospiramide Structural Diversity in Marine Bacteria. J. Am. Chem. Soc. 135, 1155-1162 (2013). https://pubs.acs.org/doi/10.1021/ja3119674
11. Roberts, A.A., Schultz, A.W., Kersten, R.D., Dorrestein, P.C., Moore, B.S. Iron acquisition in the marine actinomycete genus Salinispora is controlled by the desferrioxamine family of siderophores. FEMS Microbiol. Lett. 335, 95-103 (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC4209017/
10. Watrous, J., Roach, P., Alexandrov, T., Heath, B.S., Yang, J.Y., Kersten, R.D., van der Voort, M., Pogliano, K., Gross, H., Raaijmakers, J.M., Moore, B.S., Laskin, J., Bandeira, N., Dorrestein, P.C. Mass spectral molecular networking of living microbial colonies. Proc. Natl. Acad. Sci. U. S. A. 109, E1743-1752 (2012). https://www.pnas.org/doi/10.1073/pnas.1203689109
9. Xu, Y.*, Kersten, R.D.*, Nam, S.J., Lu, L., Al-Suwailem, A.M., Zheng, H., Fenical, W., Dorrestein, P.C., Moore, B.S., Qian, P.Y. Bacterial biosynthesis and maturation of the didemnin anti-cancer agents. J. Am. Chem. Soc. 134, 8625-32 (2012). (* - contributed equally to this work) https://pubs.acs.org/doi/10.1021/ja301735a
8. Gonzalez, D.J., Okumura, C.Y., Hollands, A., Kersten, R., Akong-Moore, K., Pence, M.A., Malone, C.L., Derieux, J., Moore, B.S., Horswill, A.R., Dixon, J.E., Dorrestein, P.C., Nizet, V. Novel phenol-soluble modulin derivatives in community-associated methicillin-resistant Staphylococcus aureus identified through imaging mass spectrometry. J. Biol. Chem. 287, 13889-13898 (2012). https://www.sciencedirect.com/science/article/pii/S002192582052961X?via%3Dihub
7. Liu, W.T., Kersten, R.D., Yang, Y.L., Moore, B.S., Dorrestein, P.C. Imaging mass spectrometry and genome mining via short sequence tagging identified the anti-infective agent arylomycin in Streptomyces roseosporus. J. Am. Chem. Soc. 133, 18010-18013 (2011). https://pubs.acs.org/doi/10.1021/ja2040877
6. Kersten, R.D., Yang, Y.L., Xu, Y., Cimermancic, P., Nam, S.J., Fenical, W., Fischbach, M.A., Moore, B.S., Dorrestein, P.C. A mass spectrometry-guided genome mining approach for natural product peptidogenomics. Nat. Chem. Biol. 7, 794-802 (2011). https://www.nature.com/articles/nchembio.684
5. Yang, Y.L., Xu, Y., Kersten, R.D., Liu, W.T., Meehan, M.J., Moore, B.S., Bandeira, N., Dorrestein, P.C. Connecting chemotypes and phenotypes of cultured marine microbial assemblages by imaging mass spectrometry. Angew. Chem. Int. Ed. Engl. 50, 5839-42 (2011). https://onlinelibrary.wiley.com/doi/10.1002/anie.201101225
4. Meier, J.L., Patel, A.D., Niessen, S., Meehan, M., Kersten, R., Yang, J.Y., Rothmann, M., Cravatt, B.F., Dorrestein, P.C., Burkart, M.D., Bafna, V. Practical 4'-phosphopantetheine active site discovery from proteomic samples. J. Proteome Res. 10, 320-319 (2011). https://pubs.acs.org/doi/full/10.1021/pr100953b
3. Kersten, R.D., Dorrestein, P.C. Metalloenzymes: Natural product nitrosation. Nat. Chem. Biol. 6, 636-7 (2010). https://www.nature.com/articles/nchembio.425
2. Kersten, R.D., Meehan, M.J., Dorrestein, P.C. Applications of Modern Mass Spectrometry Techniques in Natural Products Chemistry. Comprehensive Natural Products II, Ch.9, 389-456 (2010). https://pharmacy.hebmu.edu.cn/trywhx/resources/43/201952017112.pdf
1. Kersten, R.D. and Dorrestein, P.C., 2009. Secondary metabolomics: natural products mass spectrometry goes global. ACS Chemical Biology, 4(8), pp.599-601. https://pubs.acs.org/doi/full/10.1021/cb900187p