Our lab focuses on the study of neural connectivity and how it changes as animals develop and age. With colleagues, we developed a number of tools that permit synaptic level analysis of neural connections. In particular, we developed automated electron microscopy approaches for large scale neural circuit reconstruction. Other approaches include activity dependent uptake of fluorescent dyes, transgenic approaches to label individual nerve cells, and “combinatoric” methods (e.g., DiOlistics, Brainbow, and NPS) to label many nerve cells in the same tissue. These connectomic methods seek to make it routine to acquire neural circuit data in any nervous system.