Enzymatically Responsive Hydrogels as Cell Culture Platforms to Investigate Sex-Specific Extracellular Matrix Remodeling

Problem Statement

Currently, there is a need for creating an in vitro model for aortic valve stenosis (AVS) that can recapitulate the mechanical and biochemical properties of the in vivo tissue microenvironment. Additionally, this platform must be able to observe the sex-specific changes in cell phenotype during AVS disease progression. 

Solution

To address this need, we have developed a 3D hydrogel that will be able to mimic the microenvironment of the aortic valve leaflets. Researchers will be able encapsulate cells in the hydrogel and study the changes in cell phenotype due to external stimuli. This external stimuli is customizable through an MMP degradable matrix and ability to click on thiolated proteins to the hydrogel backbone.