Embedded Files
Problem Statement
Problem Statement
The development of standardized guidelines for designing skeletal muscle scaffolds from the decellularized extracellular matrix (dECM) is essential for their effective use in tissue engineering and regenerative medicine. With the help of these biomimetic materials, in vitro testing of novel treatments or studies about musculoskeletal diseases would be much more accurate.
There are two primary methods for creating musculoskeletal hydrogel scaffolds: laser cutting followed by decellularization, and dicing followed by decellularization, milling, and molding of skeletal muscle dECM.
Both methods aim to preserve the ECM's physiology through decellularization, a process that removes cellular material while maintaining the ECM structure. Optimizing decellularization protocols is thus a key goal of this research.
Management strategy
Management strategy
Gantt charts such as the figure above allowed our team to visualy captivate what we have been able to accomplish, and how we plan to achieve goals in the future. The team also made adjustments as the project went on in order to make the plans as efficient as possible.
Design Overview
Design Overview
Tissue harvest
Tissue harvest
Procine skeletal tissue was harvested and cut up into 0.5cm x 0.5cm cubes then stored in the -80 degrees freezer.
Decellularization
Decellularization
The tissue cubes were washed in 1% SDS for 4 days and 1 day in IPA, followed by 1 day of water wash, then collected and freezed in the -80 degrees freezer.
Lyophilization
Lyophilization
The collected frozen tissue would be lyophilized for 24 - 48hr.
Milling
Milling
The dried tissue would be milled into a fine powder using different types of filters
Digestion
Digestion
The tissue powder would be digested using pepsin and hydrochloric acid, then incubate for 24hr to solidify.
Mold formation
Mold formation
Should we talk ab this or leave it for a future direction typa page?
Page lead: Minjae Chung
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