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Image Credits: https://www.rcsb.org/structure/7QCQ
Goal
Goal
We want to make an automated nanobody discovery platform that allows for sequencing and identification of high affinity nanobody proteins to various therapeutic targets. We plan on using a microfluidic platform to ascertain protein binding and minimize costs.
Approach
Approach
We want to synthesize a nanobody library using in vitro translation. The library fragments are constructed from multiple oligo fragments designed for mRNA display such that the encoding mRNA will remain attached to the translated protein product. The target proteins are immobilized to a microfluidic flow surface via biotin attachment, and the library is flowed over it. After several washes, only nanobodies with high affinity will remain. These proteins are then sequenced.
Image Credits: https://blog.addgene.org/synthetic-nanobodies-against-membrane-proteins
Image Credits: https://www.mdpi.com/2075-4418/11/9/1530
why nanobodies?
why nanobodies?
Smaller size
Higher binding affinity
Higher thermostability
Lower immunogenicity
Image Credits: https://www.thno.org/v09p7772.htm
implications?
implications?
Better proteins for therapeutic and immunohistochemical diagnostics can help patients with protein-based diseases
Producing higher affinity and stability proteins are profitable for both patients and pharmaceutical companies
Streamline Nanobody Discovery
Watch our intro video!
Watch our intro video!
Copy of Senior Design Video.movCredit: Baylee Larson
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