There is demand for a device capable of force analysis of muscle tissue upon electrical stimulation, suspended in nutrient media which can be produced at a low cost for instructional teaching labs, such as the BENG 172 lab course.
We aimed to address the following issues with the currently used model:
Expensive to replace
Difficult to use
Damage from rust and corrosion
Muscle testing chambers have been present in biological experimentation for decades [1], often used to measure the force-length relationship of small tissue samples.
Chambers like this are distributed by a handful of manufacturers at high prices, leaving a demand for affordable and versatile muscle testing chambers for use in classrooms or research laboratory settings.
Figure 2. Experimental diagram of a muscle testing chamber. Image from [2].
Figure 3. Graph demonstrating muscle tissue force-length relationship and optimal length for force output. Image from [3].
The UCSD course BENG 170 (Bioengineering Laboratory) has a lab involving dissection of a bullfrog to extract the semitendinosis muscle, which is then placed in the muscle testing chamber to be electrically stimulate and induce twitching. Results include finding peak tension during the muscle twitch and plotting passive and active length tension curves.
The BENG 170 lab set up consists of the muscle sample resting on the chamber electrodes connected at the ends by a suture attached to an isometric force transducer on one end, and a stable rod on the other. A stimulator provides artificial pulse stimulation and an oscilloscope for data acquisition.
[1] Camila Vesga-Castro, Javier Aldazabal, Ainara Vallejo-Illarramendi, Jacobo Paredes (2022) Contractile force assessment methods for in vitro skeletal muscle tissues eLife 11:e77204 https://doi.org/10.7554/eLife.77204
[2] Rock, M. Experimental diagrams. Meghan Rocktopus. http://www.meghanrocktopus.com/diagrams-1
[3] O’Connor, W. (2015). Muscular Strength. SlidePlayer. https://slideplayer.com/slide/4746092/
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