Sukswai, N., & Khoury, J. D. (2019). Immunohistochemistry Innovations for Diagnosis and Tissue-Based Biomarker Detection. Current Hematologic Malignancy Reports, 14(5), 368–375. https://doi.org/10.1007/s11899-019-00533-9
Immunohistochemistry is an integral technique for tissue-based diagnostics and biomarker detection with broad worldwide adoption. Advances in core chemistries, antibody design, and automation have ushered unprecedented sensitivity, specificity, and reproducibility in immunohistochemistry assays. As a result, clinical immunohistochemistry assays that utilize dual-color approaches and mutation-specific antibodies provide novel tools in clinical diagnostics that until recently were in the realm of investigational research. This review provides an overview of innovations in clinical immunohistochemistry assays with emphasis on those used for patients with hematopoietic neoplasms.
Loughran, P. A., Ross, M. A., & St. Croix, C. M. (2022). Immunohistochemistry. Current Protocols, 2(9), e549. https://doi.org/10.1002/cpz1.549
Immunohistochemistry is an essential technique for the localization and measurement of proteins in cells and tissues. This article describes methods for labeling proteins in adherent and suspension cell cultures and in tissue sections. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.