The table below, show partially all the original data. The chemicals represented, are the result of unit conversion with calibration and quantification in ug/mg from the abundance of the Chromatograph.
Table 1. The white column with the complete ID of the samples, in yellow color the independent variables (week of collection of data; tree ID from 1 to 10; Treatments and control; direction of inoculation) and in blue the dependent variables : all the 20 chemicals extracted from control and treatments from the lodgepole pine trees they belong to the chemical induced defence of the tree that are produced in higher concentration under stress conditions.
Figure 8. In this is boxplot, are represented on the x-axes the chemicals from the original data, grouped in the three major terpen chemical defences (monoterpene, sesquiterpene, diterpene). On the y-axes there is the concentration of the chemical in ug/mg.
The graph is filled with the 3 treatments plus the control.
The trees are reacting more in presence of the fungi, increasing the level of monoterpenes and diterpenes. On the contrary, the level of sesquiterpenes are decreasing. That means that there is high probability that the tree is using energy to produce more some specific compounds instead of others. Within treatments, there is a slightly more difference in increasing the production of monoterpenes and diterpenes when Grosmannia clavigera is present and the range of concentration in the sesquiterpenes is slightly higher when Leptographium longiclavatum is present.
Figure 9. In this line plot I', showing in time how the different concentration of the chemical produce by the tree change in concentration but I decided to group all chemicals (by chemical structure) in three main categories of terpen: Monoterpenes, Sesquiterpenes and Diterpenes, because I want to test if there is a difference when accumulating the effect of each single chemical. On the x-axes there are the weeks when samples were collected and on the y-axes there is the change in concentration of chemicals; the graph is filled with the 3 treatments plus the control. After two weeks from inoculation in all of them there is the peaks of chemical production that decrease after 4 weeks. So, the tree is reacting more with treatments increasing dramatically the level of monoterpenes and diterpenes. There is a common response in increasing and decreasing concentration of monoterpenes in all treatments. However, there is a slight increasing level of chemicals concentration in all terpen when Grosmannia clavigera is present.
Figure 10. This figure represent the line plots for each chemical detected and their different concentration in presence of the treatments. On the x-axes, there is the time in weeks starting from the day of inoculation (at week 0) and on the y-axes the change in concentration at different scale. Chemicals are filled with the 3 treatments and the control. In overall graphs there is a common pattern of increase in concentration from the week 0 that reach in most of the cases a pick in "week 2 " and decrease until week 6. Some of them shows an interesting common pattern of increasing/decreasing in concentration (see Figure 11).
Table 2. Using one single sample t-test with a confident level of 95%, I'm showing in this table that there is a statistically evidence of chemical response from a lodgepole pine trees, after two weeks from treatments (fungi inoculation). However, only the following terpen are showing a significant correlation:
aplha-pinene, alpha-terpineol, beta myrcene, beta phellandrene, beta pinene, borneol, bornyl acetate, camphene, camphor, gamma terpinene, limonene, p-mycene, terpinen 4ol, terpinolene, gamma 3 carene.
P-values are adjusted with the method "Holm" (Rice, W. R. 1989).
Figure 11. This ten line plots represent the group of chemicals that shows a common pattern of increasing drastically the concentration in presence of treatments. All chemicals are belonging to the group of monoterpene and the highest concentrations are reached with "Beta phellandrene" that is 80 times more and "3 carene" that shows 70 times more compare to the control, after two weeks. In the x-axes is present time express in weeks, and n y-axes the changing concentration at different scale. In eight out of ten chemicals, Grosmannia clavigera shows the highest pick at week 2 compare to the other fungi; thus, the lodgepole pine trees are reacting more in presence of G.clavigera, increasing the concentration of monoterpenes and specifically increasing the concentration of "Borneol" and "Beta phellandrene" when Leptographium longiclavatum and Ophiostoma montium are present.