Osmosis Lab
Background:
Human blood, at 0.9% salt concentration, is a little less salty than seawater, which has a salt concentration of about 35 parts per thousand (3.5%).
If we take seawater as an example of a solution, the salt is called the solute (the particles that are dissolved) and the water is the solvent (the liquid that dissolves the particles).
Osmosis is the movement of a solvent across a semi-permeable membrane from an area of lower solute concentration to an area of higher solute concentration.
The water (the solvent) can move across the membrane but the dissolved solutes (the sodium and chloride ions that form salt) cannot.
In such situations, water will move across the membrane to balance the concentration of the solutes on both sides. Cells tend to lose water (their solvent) in hypertonic environments (where there are more solutes outside than inside the cell) and gain water in hypotonic environments (where there are fewer solutes outside than inside the cell). When solute concentrations are the same on both sides of the cell, there is no net water movement, and the cell is said to be in an isotonic environment.
In this lab we will test samples of potato or carrot tissue to see how much water they absorb or release in salt solutions of varying concentrations. This gives us an indirect way to measure the osmotic concentration within living cells.
Hypo=under, iso=equal, hyper=over
Problem/Purpose: What is the concentration of s in a potato cell?