Christine B. Nunez
Biotechnology M.S. Candidate | Molecular & Analytical Scientist
203-685-0089 | ChristinebNunez@gmail.com | [LinkedIn]
PROFESSIONAL SUMMARY
Biotechnology M.S. candidate with hands-on experience in molecular biology, environmental analytical chemistry, and translational research. Skilled in experimental design, PCR optimization, DNA extraction, and ELISA-based assays. Proven ability to modernize laboratory workflows, improve turnaround efficiency, and mentor developing scientists. Strong interest in R&D roles integrating molecular research with process optimization and real-world impact.
TECHNICAL SKILLS
Molecular & Cell Biology: PCR optimization, DNA extraction (Monarch/PowerSoil), cell culture, ELISA, STRING database analysis
Analytical & Environmental Methods: AAS, PLM, HACH spectrophotometry, TCLP/SPLP, wet chemistry (COD/BOD/metals)
Data & Quality: Advanced Excel (automation), LIMS (Element), SPSS/JMP, ANOVA, data visualization, QC validation, EPA/NELAC compliance, SOP development
GRADUATE RESEARCH EXPERIENCE
Graduate Thesis Research – Elms College– Spring 2025–Present
Molecular Verification of Yeast Inclusion in Equine Feeds: Optimizing PCR Protocols
• Optimized DNA extraction, purification, and PCR protocols to detect Saccharomyces cerevisiae in commercial equine feeds
• Addressed false-negative amplification challenges caused by complex feed matrices by comparing multiple polymerases and DNA cleanup kits
• Developed a methodological workflow to improve accuracy and reproducibility in microbial verification for processed feeds
• Provided insights toward potential quality control pathways for commercial equine probiotic products
• Presented findings via poster at Pioneer Valley Microbiology Symposium (March 2026) and Experiential Learning Showcase (May 2025)
Graduate Research – Elms College– Spring 2025–Present
Methylparaben Protein–Protein Interactions in Human Ductal Breast Carcinoma Cell Line ZR-75-1
• Conducted ELISA assays on ZR-75-1 ductal carcinoma cells dosed with methylparaben to quantify Hsp90 and Tau (MAPT) expression
• Integrated experimental data with STRING protein–protein interaction analysis and literature to identify mechanistic pathways affected by endocrine-disrupting compounds
• Synthesized findings to contextualize molecular interactions in tumor microenvironment signaling
• Scheduled for poster and slide presentation at Experiential Learning Showcase, May 2026 (upcoming)
PROFESSION EXPERIENCE
Laboratory Analyst – Enviromed, Meriden, CT – April 2024–Present
• Ensured 100% compliance in SOPs and volumetric dilutions during NELAC/TNI audits
• Reduced contamination and sample re-runs by re-engineering glassware decontamination and hood hygiene
• Automated Excel-based data analysis with NIST-traceable calibrations, increasing reporting accuracy and efficiency
• Delivered same-day reporting for 24-hour TAT lead clearance samples, improving prior lab throughput
• Manage procurement and mentor new analysts in analytical methods, EPA-compliant documentation, and ELPAT proficiency testing
Chemist / Analyst – Complete Environmental Testing, Stratford, CT – May 2022–April 2024
• Led TCLP/SPLP extractions and wet chemistry assays with full QC validation, increasing throughput by ~30% through digital workflow restructuring
• Performed matrix-specific extractions and validated results via Matrix Spikes, Blanks, and Calibration Verifications (ICV/CCV)
• Utilized LIMS (Element) for structured data tracking, reporting integrity, and analytical accuracy across multiple analytes
LEADERSHIP & MENTORSHIP
• Train and guide new laboratory personnel using structured troubleshooting methodology
• Foster independent analytical thinking by encouraging guided problem-solving
• Recognized for clear instruction, supportive mentorship, and developing technical confidence in team members
POSTERS & PRESENTATIONS
Experiential Learning Showcase, Elms College – May 2025
Equine Probiotics: Comparison of Polymerase Performance in Molecular Detection (Poster)
Pioneer Valley Microbiology Symposium – March 2026
Molecular Verification of Yeast Inclusion in Equine Feeds: Optimizing PCR Protocols (Poster)
Experiential Learning Showcase, Elms College – May 2026 (upcoming)
Methylparaben Protein–Protein Interactions in Human Ductal Breast Carcinoma Cell Line ZR-75-1 (Poster & Presentation)
University of Bridgeport – Faculty Research Day – Spring 2017
Hydroponics: Comparison of Culture Systems and Nutrient Levels for Growing Arabidopsis thaliana (Poster)
University of Bridgeport – Faculty Research Day – Spring 2015
Monk Parakeets: Tolerance and Territoriality (Poster)
EDUCATION
M.S. Biotechnology – Elms College – Expected May 2026 | GPA: 3.9
Certificates: Cell Culture (2025), ELISA (2025)
B.S. Biology & Psychology – University of Bridgeport – December 2018 | GPA: 3.140
Honors: Beta Beta Beta Biological Honor Society