DST-FIST Protein Characterization Facility

The basic principle of Circular Dichroism (CD) is the measurement of the difference in absorbance between left- and right-circularly polarized light as it passes through an optically active sample. CD spectroscopy is commonly used to study the structure of chiral molecules, such as proteins, by detecting their characteristic secondary and tertiary conformations. The resulting CD spectrum plots the wavelength-dependent difference in absorbance, providing insights into molecular conformation and electronic transitions.

Room No: 318, Block 1

The MicroCal PEAQ Malvern ITC (Isothermal Titration Calorimeter) measures the heat released or absorbed during molecular interactions, providing detailed insights into binding affinity, stoichiometry, and thermodynamics.

Room No: 318, Block 1

The Jasco V-760 UV-Vis Spectrophotometer is used for precise absorbance and transmittance measurements across the ultraviolet and visible light spectra, facilitating the analysis of molecular concentration, chemical composition, and reaction kinetics.

Room No: 318, Block 1

The PerkinElmer FTIR (Fourier Transform Infrared) spectrometer is used to identify chemical compounds and analyze molecular structures by measuring the absorption of infrared light across different wavelengths.

Room No: 318, Block 1

The Malvern Zetasizer DLS (Dynamic Light Scattering) measures the size distribution, zeta potential, and molecular dynamics of nanoparticles and colloidal suspensions, aiding in material characterization and stability analysis.

Room No: 318, Block 1

The QuantStudio Flex by Applied Biosystems is a real-time PCR system used for high-precision gene expression analysis, genotyping, and other molecular biology applications.

Room No: 318, Block 1

The Bio-Rad ChemiDoc MP Imaging System is a versatile platform used for capturing high-resolution images of chemiluminescent, fluorescence, and colorimetric assays in molecular biology research. It enables precise quantification and analysis of protein, nucleic acid, and cell-based experiments, offering advanced image processing and data analysis tools for a wide range of applications.

Room No: 114, Block 2

The BioTek Synergy H1 is a multi-mode microplate reader used for a wide range of applications, including absorbance, fluorescence, and luminescence assays, providing versatile and high-throughput detection in research and diagnostics.

Room No: 318, Block 1

A lyophilizer, also known as a freeze dryer, removes moisture from samples by freezing them and then applying a vacuum, thereby preserving delicate biological and pharmaceutical materials. Used for organic samples or for samples between 12-48 hours

Block 1. Room no:112

A lyophilizer, also known as a freeze dryer, removes moisture from samples by freezing them and then applying a vacuum, thereby preserving delicate biological and pharmaceutical materials. Used for tissue samples or for samples greater than 48 hours.

Block 2, Room no: 218

The Thermo Scientific NanoDrop One is a highly sensitive spectrophotometer designed for quick and accurate quantification and quality assessment of nucleic acids, proteins, and other biomolecules using just a small sample volume.

Room No: 114, Block 2