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OVERVIEW
OVERVIEW
The Department of Biotechnology at IIT Madras and DST-FIST Advanced Bioimaging Facility in collaboration with the India Bioimaging Consortium is organizing a specialized hands-on-training workshop on advanced fluorescence microscopy techniques and image analysis designed for Ph.D. scholars, Post-Doctoral researchers from academic institutions and industry working in bioscience, bioengineering, biomedical and medical sciences, pharmaceutical sciences, and allied fields.
This workshop will offer a comprehensive blend of theoretical lectures and practical training in both fundamental and advanced microscopy techniques. It is designed for small, focused groups, and will involve active learning through expert lectures from internationally recognized faculty and technical scientists, guided experiments, and hands-on exposure to sophisticated microscopy systems on a variety of fluorescence-based techniques.
Training Curriculum:
Fundamentals of Light Microscopy and Immunofluorescence
Live Cell Imaging & Time-lapse Imaging
Förster Resonance Energy Transfer (FRET)
Fluorescence Recovery After Photobleaching (FRAP)
Expansion Microscopy (ExM)
Total Internal Reflection Fluorescence (TIRF)
Quantitative Image Analysis and Interpretation
Salient Features:
Hands-on instructions on wide-field and confocal microscopy systems
Networking and mentoring opportunities with internationally recognized faculty and microscopy support specialists
Individual problem solving, discussion and trouble-shooting
Free access to training toolkit, reference guides, and image analysis software
Software demonstrations and data analysis sessions
Certificate of participation from IIT Madras
Organizer:
Dr. Shantanu Pradhan
Assistant Professor
Department of Biotechnology
Bhupat & Jyoti Mehta School of Biosciences
Indian Institute of Technology Madras
Phone: 044-2257-4125
Email: spradhan@smail.iitm.ac.in
Coordinators:
Dr. Srividhya Ravichandran
Shreaya Bhaskar
Sneha Sathapathi
Danisha M
Sasirekha Mathaiyan
Madhankumar S
OBJECTIVES
OBJECTIVES
Conceptual understanding of basic principles of fluorescence, fluorophore properties, optical path of a fluorescence microscope, widefield vs. confocal vs. super-resolution microscopes
Practical microscope skills involving microscope set up and image acquisition from fixed and live samples, with optimized parameters to minimize photobleaching and phototoxicity
Sample preparation and labeling involving appropriate selection of fluorescent dyes/probes/proteins for specific biological questions, avoiding common artefacts and protocol adjustment
Image processing and analysis involving basic image processing tools (ImageJ/FIJI), deconvolution, quantitative analyses of colocalization, intensity across multiple dimensions (time, z, wavelength), and scientific presentation of images and graphs
Application and experimental design to choose the right microscopy platform/technique to answer biological questions of interest, including controls and replicates, and interpret fluorescence images to draw biological conclusions
IMPORTANT DATES & DETAILS
IMPORTANT DATES & DETAILS
Number of seats: 15 (students/postdocs/technical staff/young faculty)
Minimum qualification: Masters in any branch of Life Science/Engineering/Allied areas
Abstract submission deadline: March 31, 2026
Abstract submission link: https://forms.gle/nEHE8PAuPnQENAik7
Registration deadline: April 15, 2026
Registration link: Will be sent to the selected participants via email.
Registration fees: Rs. 9,440 (Rs. 8,000/- + 18% GST) (without accommodation)
Rs. 11,800/- (Rs. 10,000/- + 18% GST) (with hostel accommodation)
(Fees includes registration, food, workshop literature and other materials)
Guest House accommodation is limited and will be provided on request.
SPEAKERS & TRAINERS
SPEAKERS & TRAINERS
Dr. Shantanu Pradhan,
IIT Madras
Dr. Mamata Bangera,
IIT Madras
Dr. Richa Karmakar,
IIT Madras
Dr. Krishanu Ray,
BITS Pilani Hyderabad
Dr. Sreelaja Nair,
IIT Bombay
Dr. Sudipta Maiti,
BITS Pilani Hyderabad
Dr. Deepak Nair,
IISc Bangalore
Dr. Priti Agarwal,
NCBS Bangalore
Dr. Sveta Chakrabarti,
Manipal Institute of Regenerative Medicine, MAHE
Dr. Bibhu Ranjan Sarangi,
IIT Palakkad
Dr. Ganesh Kadasoor,
Evident Scientific
Dr. Sebastian Raja, Leica Microsystems
Shiva Prasad,
DSS Imagetech
PARTNERS
PARTNERS
SPONSORSHIP
SPONSORSHIP
Diamond sponsor - Rs. 50,000/- (Dedicated technical presentation and demonstration time, Stall with instrument demo, logos on banners & other Workshop materials )
Gold sponsor - Rs. 25,000/- (Stall with instrument demo, logos on banners & other Workshop materials)
Silver sponsor - Rs. 10,000/- (Logos on banners & other Workshop materials)
CONTACT
CONTACT
Email: spradhan@smail.iitm.ac.in, advancedimagingfacility@smail.iitm.ac.in
Phone: 044-2257-4125, 044-2257-5097
Venue: Department of Biotechnology (Block 1), Bhupat & Jyoti Mehta School of Biosciences, IIT Madras, Guindy, Chennai, Tamil Nadu 600036
DST-FIST Advanced Bioimaging Facility hosted within BIO-SAIF (Sophisticated Analytical Instrumentation Facility) is a modern core facility equipped with state-of-the-art widefield (Olympus IX83) and confocal (Olympus FV3000) systems, providing an ideal setting for hands-on training in advanced microscopy techniques.
Accommodation: Outstation participants will be accommodated in IIT Madras hostel. Invited speakers will be accommodated in Taramani Guest House (https://tgh.iitm.ac.in/). Guest House accommodation for participants will be arranged upon request.
Information for Participants:
Bring your own laptops for image analysis modules.
Install FIJI (ImageJ) software prior to arrival.
You may bring some of your own samples for imaging.
Carry USB drives for data backup.
Prepare 1–2 biological questions relevant to their research for discussion during interactive sessions.
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