Epigenetic modifications are involved in the development of chronic conditions such as obesity. DNA methylation is one type of three epigenetic modifications affected by nutritional factors. After methylation sequencing, methylation levels of individual genes need to be verified. One method to verify methylation of individual genes is to use Polymerase Chain Reaction (PCR) to amplify genomic DNA samples digested by methylation dependent restriction enzyme MspI, methylation sensitive restriction enzyme HpaII, or the combination of the two enzymes. This method is applied to check the change of methylation levels of Peroxisome proliferator-activated receptor delta (PPARδ) in conditions that mimic the development of obesity. The results indicate general methylation levels of the PPARδ gene decrease when saturated fatty acid palmitic acid is applied, while the methylation levels increase when polyunsaturated fatty acid linoleic acid is applied in comparison to the control. Since PPARδ is involved in adipogenesis, these results are consistent with the observation that high intake of saturated fatty acids is a risk factor of obesity.

Dr. Li, yi.li@health.slu.edu, has been an extraordinary mentor over the last year by sparking an interest in the field of biochemical research, while simultaneously developing Kyle's skills as a researcher. By encouraging Kyle to find the solutions to problems that arise rather than ask for answers, Dr. Li has reignited his curiosity in learning. He promoted autonomy and encouraged Kyle to take charge in training new research assistants. Kyle is fortunate to have taken the opportunities that he has provided, and he understands that this research experience has and will have effects that extend far beyond my resume.