Publications and Patents

Patents

Patents Granted:

  1. "In-line digital holographic microscope and a method of in-line holographic microscopy”, US Patent (Patent Number: 8194124). (web link)

  2. High Sensitivity Temporal Focusing Widefield Multiphoton Endoscope Capable of Deep Imaging", US Patent (Patent number: 9867525). (web link)

  3. 'Phase Cancellation Microscopy", September 2021, US patent (patent number 11255656) (web link)

Patent applications in process:

  1. "Diffraction grating based common path interferometer for imaging Fourier transform scpectroscopy", July 2016, US patent application US2016/013366. (web link)

  2. "A Digital Holographic Microscopy System and a Method of Digital Holographic Microscopy", WIPO Patent Application WO/2009/148407. (web link)


Book(s) Chapter(s)

  1. Chapter Title: "Digital Reflection Holography and Applications" (web link)

Book Title: "Digital Holography for MEMS & Micro Systems Metrology" (Edited by Anand Asundi) (web link)

Publisher: John Wiely, UK (September 2011)

2. Chapter Title: "Optical Instrumentation Design for Fluorescence Lifetime Spectroscopy and Imaging"

Book Title: "Fluorescence Lifetime Spectroscopy and Imaging"(Edited by Laura Marcu, Paul French, and Daniel Elson)

Publisher: Taylor & Francis Books, Inc. (July 2014) (web link)


Research Papers in Peer-Reviewed International Journals

  1. V.R. Singh and P.T.C. So, “Photon reassignment method for structured illumination fluorescence microscopy for 3D tissue imaging”, manuscript in preparation.

  2. V.R. Singh and P.T.C. So, “Spectral un-mixing method of neuronal images using Poisson noise based maximum likelihood estimation”, manuscript in preparation.

  3. A.B. Roberts, J. Zhang, V.R. Singh, M. Nikolic, E. Moeendarbary, R.D. Kamm, P. So, G. Scarcelli, "Tumor cell nuclei soften during transendothelial migration", J. Biomechanics, Vol. 121, 110400, 2021. (web link)

  4. Y.-H. Tsai, C.-W. Liu, W.-K. Lin, C.-S. Wang, C.-H. Chiang, V.R. Singh, P. So, C.-F. Chou, S.-W. Chu, "Two-photon microscopy at >500 volumes/second", bioRxiv, 2020. (web link)

  5. V.R. Singh, Y.A. Yang, H. Yu, R.D. Kamm, Z. Yaqoob, P.T.C. So, "Studying nucleic and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy", Nature Communications, 10, Article number: 3652, 2019. (web link)

  6. D.N. Wadduwage, J. Kay, V.R. Singh, O. Kiraly, M.R. S.-Jackson, J. Rajapakse2, B.P. Engelward, P. So, “Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice”, Scientific Report, 8, 12108, 2018. (web link)

  7. A. Ahmad, V. Dubey, V.R. Singh, J.-C. Tinguley, C.I. Øie, D.L. Wolfson, D.S. Mehta, P.T C. So and B.S. Ahluwalia, "Quantitative phase microscopy of red blood cells during planner trapping and propulsion", Lab on a Chip, 18, 3025, 2018. (web link)

  8. W.-T. Lin, C.Y. Lin, V.R. Singh, and Y. Luo, "Speckle illumintion holographic non-scanning (SHIN) fluorescence endoscopy", Journal of Biophotonics, e201800010, 2018.(web link)

  9. D.N. Wadduwage, V.R. Singh, H. Choi, Z. Yaqoob, J.A. Heemskerk, P. Matsudaria, P. So, "A novel common-path interferometer for full-field imaging Fourier tranform spectroscopy", Optica, Vol. 4(5), 546-556, 2017. (web link)

  10. R. Zhou, D. Jin, P. Hosseini, V.R. Singh, Y.-H. Kim, C. Kuang, R.R. Dasari, Z. Yaqoob, P.So, "Modeling the depth-sectioning effect in dynamic speckle-field interferometric microscopy", Optics Express, Vol. 25(1), 130-143, 2017. (web link)

  11. P.-H. Wang, V.R. Singh, J.-M. Wong, Y. Luo, "Non-axial-scanning multi-focal confocal microscopy with multiplexed volume holographic gratings", Optics Letters, Vol. 42 (2), 346-349, 2017. (web link)

  12. C.Y. Lin, W.-T. Lin, H.-S. Chen, J.-M. Wong, V.R. Singh, Y. Luo, "Talbot illumination holographic fluorescence endoscopy for optically sectioned multi-plane imaging", Optics Letters, Vol. 14(2), 344-347, 2016. (web link)

  13. X. Zhai, W.-T. Lin, H.-H. Chen, P.-H. Wang, L.-H. Yeh, J.-C. Tsai, V.R. Singh, and Y. Luo, "In-line digital holographic imaging in volume holographic microscopy", Optics Letters, Vol. 40(23), 5542-5545, 2015. (web link)

  14. H.-H. Chen, V.R. Singh, L. Yuan, "Speckle-based volume holographic microscopy for optically sectioned multi-plane fluorescent imaging", Optics Express, Vol. 23(6), 7075-7084, 2015. (web link

  15. *L. Yuan, *V.R. Singh, D. Bhattacharya, E.Y.S. Yew, P. Matsudaira, P.T.C. So, G. Barbastathis, "Talbot Holographic Illumination Non-scanning (THIN) Fluorescence Microscopy", Laser & Photonics Reviews, Vol. 8(5), L71-L75, 2014. (*Shared first author) (web link)

  16. *J.W. Cha, *V.R. Singh, K.H. Kim, J. Subramanian, E. Nedivi, P. So, "Reassignment of Scattered Emission Photons in Miltifocal Multiphoton Microscopy", Scientific Reports (Nature Publishing), Vol. 4, Article number: 5153, 2014. (*Shared first author) (web link)

  17. M.R.S. Jackson, O. Kiraly, J.E. Kay, L. Na, E.A. Rowland, K.E. Winther, D.N. Chow, T. Kimoto, T. Matsuguchi, V.S. Jonnalagadda, V.I. Maklakova, V.R. Singh, D.N. Wadduwage, J. Rajapakse, P.T. C. So, L.S. Collier, B.P. Engelward, "Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo", PLOS Genetics, Vol. 10, Issue 6, e1004299, 2014. (web link)

  18. G. Hegde, V.R. Singh, A. Asundi, "Digital Holographic Microscopy for MEMS/MOEMS Device Inspection and Complete Characterization (Review Article)", Journal of Indian Institute of Science, Vol. 93(1), pp. 2013. (web link)

  19. D. Bhattacharya, V.R. Singh, C. Zhi, P.T.C. So, P. Matsudaira, G. Barbastathis, "Three dimensional HiLo-based structured illumination for a Digital Scanned Laser Sheet Microscope (DSLM) in thick tissue imaging", Optics Express, Vol. 20, Issue 25,27337-27347, 2012. (web link)

  20. V.R. Singh, H. Choi, E.Y.S. Yew, D. Bhattacharya, L. Yuan, C.J.R Sheppard, J.C. Rajapakse, G. Barbastathis, P.T.C. So, "Improving Signal-to-noise ratio of structured light microscopy based on photon reassignment", Biomedical Optics Express, Vol. 3, Issue 1, 206-214, 2012. (web link)

  21. K. Dev, V.R. Singh, A. Asundi, "Full-field Phase Modulation Characterization of Liquid Crystal Spatial Light Modulator using Digital Holography", Applied Optics, Vol. 50, Number 11, 1593-1600, 2011. (web link)

  22. V.R. Singh, E. Darakis, G. Hegde, and A. Asundi, “A new methodology for pixel size retention in lensless digital holographic microscopy applied to micro-particles analysis”, Journal of optics A: Pure and Applied Optics, Vol. 13, Issue 3, 035704, 2011. (web link)

  23. M. Olfatnia, V.R. Singh, T. Xu, J. Miao, and L.S. Ong, "Analysis of Vibration Modes of Piezoelectric Circular Microdiaphragms", Journal of Micromechanics and Microengineering, Vol. 20, Number 8, 085013, 2010. (web link)

  24. B. Jayaraman, V.R. Singh, A. Asundi, G.M. Hegde and N. Bhat, “Thermo-mechanical Characterization of Surface-micromachined Microheaters using In-line Digital Holography”, Measurement Science and Technology, Vol. 21, Issue 1, 015301 (10pp), 2010. (web link)

  25. V.R. Singh and A. Asundi, “In-line digital holography for dynamic metrology of MEMS”, Invited Paper, Chinese Optics Letters, Vol. 7, Issue 12, pp. 1117, 2009. (web link)

  26. W. Qu, O.C. Choo, V.R. Singh, Y. Yu, and A. Asundi, “Quasi physical phase compensation in digital holographic microscopy”, Journal of Optical Society of America (JOSA)-A, Vol. 26, Issue 9, pp. 2005, 2009. (web link)

  27. V.R. Singh, G. Hegde, A. Asundi, “Particle field imaging using digital in-line holography”, Current Science, Vol. 96, No. 3, pp. 391-397, 2009. (Web link)

  28. V.R. Singh, J. Miao, Z. Wang, G. Hegde, and A. Asundi, “Dynamic Characterization of MEMS diaphragm using time averaged in-line digital holography”, Optics Communications, Vol. 280, Issue 2, pp. 285, 2007. (Web link)

  29. A. Asundi and V.R. Singh, “Amplitude and Phase Analysis in Digital Dynamic Holography”, Optics Letters, Vol. 31, No. 16, pp. 2420, 2006. (Web link)

  30. A. Asundi and V.R. Singh, “Time averaged in-line digital holographic interferometry for vibration analysis”, Applied Optics, Vol. 45, No. 11, pp. 2391, 2006. (Web link)

  31. V.R. Singh and Anand Asundi, “Vibration analysis of membrane by time-average in-line digital holographic interferometry”, Key Engineering Materials, Vol. 326-328, pp. 23, 2006. (Web link)

  32. A. Asundi and V.R. Singh, “Sectioning of Amplitude Images in Digital Holography”, Measurement Science and Technology, Vol. 17, pp 75, 2006. (Web link)

  33. A. Asundi, and V.R. Singh, “Circle of Holography – Digital In-line Holography for Imaging, Microscopy and Measurement” Journal of Holography and Speckle, Vol. 3, No2, pp. 106-111, 2006. (Web link)


Research Papers in International Conferences Proceedings

  1. V.R. Singh, J.C. Rajapakse, H. Yu, P.T.C. So, "Intensity normalization of two-photon microscopy images for liver fibrosis analysis", Proc. SPIE, Vol. 7903, pp. 79030P, 2011. (Web link)

  2. V.R. Singh, S. Lui, and A. Asundi, “Digital reflection holography based systems for MEMS measurements”, Proc. SPIE, Vol. 7718, pp. 77180Z, 2010. (Web link)

  3. M. Olfatnia, V.R. Singh, T. Xu, J.M. Miao, L.S. Ong, "Response of Piezoelectric Circular Microdiaphragm Sensors in Higher Frequency Modes, IEEE Sensors, 2662-2665, 2010. (Web link)

  4. K. Dev, V.R. Singh, A. Asundi, "Full-field TN-LCSLM phase modulation characterization using digital holography", Proc. SPIE, Vol. 7775, pp. 777519, 2010. (Web link)

  5. V.R. Singh, S. Lui, and A. Asundi, “Compact Handheld Digital Holograp hic Microscopy System Development”, proc. SPIE, Vol. 7522, pp. 75224L, 2009. (Web link)

  6. V.R. Singh, E. Darakis, and A. Asundi, “Dynamic imaging of Micro-particles in 3D Using Lensless In-line Digital Holographic Microscopy”, Proc. of SPIE, Vol. 7522, pp. 75221G, 2009. (Web link)

  7. T.R. Lewis, W. Qu, O.C. Chee, V.R. Singh, and A. Asundi, "Quantitative analysis of live cells using digital holographic microscopy", Proc. SPIE, Vol. 7522, pp. 75222P, 2009. (Web link)

  8. C.P. Lee, Y.P. Chia, V.R. Singh, A. Asundi, X.J. Khoo, K.L. Tay, and J. Zhou, "Digital holographic display", Proc. SPIE, Vol. 7522, pp. 75225S, 2009. (Web link)

  9. W. Qu, O.C. Chee, Y. Yu, V.R. Singh, and A. Asundi, "Physical phase compensation in digital holographic microscopy", Proc. SPIE, Vol. 7522, pp. 75224P, 2009. (Web link)

  10. A. Asundi, and V.R. Singh, “Digital Holography for MEMS Applications”, Invited Paper, Novel Techniques in Microscopy, OSA Technical Digest (CD) (Optical Society of America, 2009), paper JTuA2, 2009. (Web link)

  11. A. Asundi, V.R. Singh, W.J. Qu, T. Khanam, "Compact digital holographic microscope and applications", Conference on Lasers and Electro-Optics/Pecific Rim (CLEOPR), Shanghai, China (Optical Society of America), Paper: TuE3_2, 2009. (Web link)

  12. V.R. Singh, O.C. Chee, E. Sim, and A.Asundi, “Characterisation of laser marks using digital holographic microscopy”, Proc. SPIE, Vol. 7155, pp. 715509-1, 2008. (web link)

  13. V.R. Singh, A. Anderi, C. Gorecki, L. Nieradko, and A. Asundi, “Characterization of MEMS cantilevers using lensless digital holographic microscope”, Proc. SPIE, Vol. 6995, pp. 69950F-1, 2008. (web link)

  14. O.C. Chee, V.R. Singh, E. Sim, and A. Asundi, “Development of simple user-friendly commercial digital holographic microscope”, Proc. of SPIE, Vol. 6912, pp. 69120V-1, 2008. (web link)

  15. A. Asundi and V.R. Singh, “Digital Holography—A new paradigm in Imaging, Microscopy and Metrology”, Invited Paper, Presented in International Conference on Optics and Laser Applications (ICOLA) Indonesia, in September 2007. (web link)

  16. E. Darakis, V.R. Singh, A. Asundi, J.J. Soraghan, “Phase measurement errors due to holographic interferograms compression”, Proceedings of SPIE, Presented in optical metrology conference of SPIE in Munich, Germany in June 2007, Vol. 6616, pp. 661612-1, 2007. (web link)

  17. V.R. Singh, A. Asundi, and M. Jianmin, “Double exposure time averaged in-line digital holography”, Proceedings of SPIE, Presented in optical metrology conference of SPIE in Munich, Germany in June 2007, Vol. 6616, pp. 661630-1, 2007. (web link)

  18. V.R. Singh and A. Asundi, “Advances in dynamic metrology using in-line digital holographic Interferometry”, Proceeding of SPIE, Invited Paper, Presented in Annual conference of SPIE in San Diego, USA in August 2006, Vol. 6293, pp. 629306-1, 2006. (Web link)

  19. V.R. Singh, G. Hegde and A. Asundi, “Digital in-line holography for dynamic micrometrology”, Proceedings of SPIE, Presented in Photonics Europe, France in April 2006, Vol. 6188, pp. 618803-1, 2006. (Web link)

  20. V.R. Singh and A. Asundi, “Amplitude contrast image enhancement in digital holography for particles analysis”, Proceedings of SPIE, Proceedings of SPIE, Presented in annual conference of SPIE in San Diego, USA in August 2005, Vol. 5878, pp. 587817-1, 2005. (Web link)

  21. V.R. Singh and A. Asundi, “Digital in-line holography for microscopic applications”, Proceeding of International Congress of experimental Mechanics (ICEM 05), Presented in New Delhi, India in September, 2005, pp. 186, 2005.

  22. C. Shakher, S. Mirza, V.R. Singh, M. Hossain and R.S. Sirohi, “Different preprocessing and wavelet transform based filtering technique to improve Signal-to-noise ratio in DSPI fringes”, Wolfgang Osten (Ed.), Fringe 2005, The 5th International Workshop on Automatic Processing of Fringe Pattern, Germany, pp. 142, 2005. (web link)


Invited Papers / Talks

  1. Invited Talk: "Studying nucleic and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy”, Northeast Symposium on Bimedical Optics 2018, Organized by Wellman Center for Photomedicine, Massachusetts General Hospital. (Web link)

  2. Invited Talk: "3D Fluorescence Microscopy systems development”, guest lecture (October 30, 2013), Jaypee Institute of Information Technology (JIIT), Noida, India. (Web link)

  3. Invited Paper: “In-line digital holography for dynamic metrology of MEMS”, Chinese Optics Letters, Vol. 7, Issue 12, pp. 1117, 2009. (web link)

  4. Invited Talk: “Digital Holography for MEMS Applications”, Novel Techniques in Microscopy, OSA Technical Digest (CD) (Optical Society of America, 2009), paper JTuA2, Presented at Vancouver, Canada, 2009. (Web link)

  5. Invited Talk: “Digital Holography—A new paradigm in Imaging, Microscopy and Metrology”, Presented in International Conference on Optics and Laser Applications (ICOLA) Indonesia, in September 2007. (web link)

  6. Invited Paper: “Advances in dynamic metrology using in-line digital holographic Interferometry”, Proceeding of SPIE, Presented in Annual conference of SPIE in San Diego, USA in August 2006, Vol. 6293, pp. 629306-1, 2006. (Web link)


Doctor of Philosophy (Ph.D.) Thesis

Title of the thesis: “Digital In-line Holography for Dynamic Applications”

Awarded by: Nanyang Technological University, Singapore

Year of award: 2008

[Click here to download the thesis]

Updated on Jul 29, 2019