SMTC@BostonChildren's 

Single Molecule Fluorescence Microscopy

• Single molecule FRET and PIFE

• Single molecule pulldown (SiMPull)

• Single molecule localization microscopy and tracking

Single Molecule Tools for Cell Dynamics and Profiling

• Genome Oligopaint via Local Denaturation (GOLD) FISH

• Very fast (vf) CRISPR

• Multiplexed Error-Robust Fluorescence in situ Hybridization (MERFISH)

GRC Genome Architecture in Cell Fate and Disease

First Salamanca International Advanced Biophysics and Structural Biology Course

8th Nucleic Acids Conference

VAI Epigenetics Symposium 

Genome Architecture and Function Summer School and Workshop

Genome Engineering: CRISPR Frontiers at Cold Spring Harbor Laboratory

30th International Workshop on “Single Molecule Spectroscopy and Super-resolution Microscopy

2nd Spatial Genome Organisation Conference

70th Annual Meeting, Biophysical Society

• Two-color single molecule total internal reflection microscope (x2)

• Four-color single molecule total internal reflection microscope (x2)

• MERFISH microscope (x2)

• Single molecule localization microscope for super-resolution

• Three-color single molecule total internal reflection microscope for live cell imaging

• High speed atomic force microscope

• LUMICK C-Trap

• Picoquant LUMINOSA single molecule confocal microscope

• PROTEINA SPID (Single-molecule Protein Interaction Detection) microscope

Software tools

• smCamera

Reference samples

• smFRET

Holliday junction

Cas9 DNA unwinding

• smPIFE

T7 transcription initiation

RecA filament dynamics

• vfCRISPR

caged guide RNA

• GOLDFISH

FISH probes for standard target sites

• MERFISH

Standard reagents

Microscope designs

Measurement protocols

• A structured guide to SMTC videos

• Training activities

• Summer School

• Boot Camp

• Imaging Innovation Workshop

• Mini-grants

Academic partners

• Shan Zha (Columbia University)

• Alistair Boettiger (Stanford University)

• Catherine Royer (Rensselaer Polytechnique University)

• Richard Fishel (Ohio State University)

• Sangjin Kim (University of Illinois at Urbana-Champaign)

• James Berger (Johns Hopkins University)

• James Shorter (University of Pennsylvania)

• Amy Gladfelter (Duke University)

• Yang Gao (Rice University)

• Sanjeevi Sivasankar (University of California at Davis)

• Timothy Springer (Harvard Medical School)

• Shizhen Wang (University of Missouri, Kansas City)

• Ken Lau (Vanderbilt University)

• Jeffrey Spraggins (Vanderbilt University)

Industrial partners

• LUMICKS

• Oxford NanoImaging

• Exciting Instruments

• Bruker

• PicoQuant

• Vizgen

• PROTEINA

Single molecule biophysics labs in the United States (incomplete)

• Alabama

  • Hui-Ting Lee (University of Alabama at Birmingham)

• Arizona

  • Steve Presse (Arizona State University)

  • Rizal Hariadi (Arizona State University)

  • Marcia Levitus (Arizona State University)

• California

  • Steven Chu (Stanford University)

  • Bianxiao Cui (Stanford University)

  • Alistair Boettiger (Stanford University)

  • Sanjeevi Sivasankar (University of California at Davis)

  • Ahmet Yildiz (University of California at Berkeley)

  • Carlos Bustamante (University of California at Berkeley)

  • Xavier Darzacq (University of California at Berkeley)

  • Ke Xu (University of California at Berkeley) 

  • Douglas Smith (University of California at San Diego)

  • Shimon Weiss (UCLA)

  • W.E. Moerner (Stanford University)

  • Shannon Yan (Stanford University)

  • Omar Saleh (University of California at Santa Barbara)

  • Michael Stone (University of California at Santa Cruz)

  • Pallav Kosuri (Salk Institute)

  • Ashok Deniz (Scripps Institute)

  • Zev Bryant (Stanford University)

  • Yale Goldman (University of California at Davis)

  • Alexander Dunn (Stanford University)

  • Fabien Pinaud (University of Southern California)

  • Gheorgeh Chistol (Stanford University)

  • Shasha Chong (California Institute of Technology)

  • Vu Nguyen (University of California at San Diego)

• Colorado

  • David Nesbitt (University of Colorado, Boulder)

  • Thomas Perkins (University of Colorado, Boulder)

  • Diego Krapf (Colorado State University)

  • Timothy Stasevich (Colorado State University)

• Connecticut

  • Yongli Zhang (Yale University)

  • Moitrayee Bhattacharyya (Yale University)

• Georgia

  • Harold Kim (Georgia Institute of Technology)

  • Cheng Zhu (Georgia Institute of Technology)

  • Khalid Salaita (Emory University)

• Illinois

  • Jingyi Fei (University of Chicago)

  • Yann Chemla (University of Illinois at Urbana-Champaign)

  • Ido Golding (University of Illinois at Urbana-Champaign)

  • Sangjin Kim (University of Illinois at Urbana-Champaign)

  • Paul Selvin (University of Illinois at Urbana-Champaign)

  • Reza Vafabakhsh (Northwestern University)

  • John Marko (Northwestern University)

  • Christy Landes (University of Illinois at Urbana-Champaign)

  • Allison Squires (University of Chicago)

  • Ronald Rock (University of Chicago)

• Indiana

  • Matthew Bochman (Indiana University)

• Iowa

  • Maria Spies (University of Iowa)

• Kansas

  • Erik Holmstrom (University of Kansas, Lawrence)

• Kentucky

  • Prakash Shrestha (University of Kentucky)

• Maine

  • Samuel Hess (University of Maine)

• Maryland

  • Jie Xiao (Johns Hopkins University)

  • Bin Wu (Johns Hopkins University)

  • Keir Neuman (National Institutes of Health)

  • Minjoung Kyoung (University of Maryland, Baltimore County)

  • Rodrigo Maillard (Georgetwon University)

  • Daniel Larson (National Institutes of Health)

  • Hoi-Sung Chung (National Institutes of Health)

  • Quan Wang (National Institutes of Health)

  • Inhee Chung (George Washington University)

  • Sarah Woodson (Johns Hopkins University)

  • Carl Wu (Johns Hopkins University)

  • William Huang (Johns Hopkins University)

  • Thomas Graham (Johns Hopkins University)

• Massachusetts

  • Xiaowei Zhuang (Harvard University)

  • Mark Williams (Northeastern University)

  • Jeff Gelles (Brandeis University)

  • Wesley Wong (Harvard Medical School)

  • Joseph Loparo (Harvard Medical School)

  • Meni Wanunu (Northeastern University)

  • Gabriela Schlau-Cohen (MIT)

  • Chunte (Sam) Peng (MIT)

  • Ashlee Carter (Amherst College)

  • Anders Hansen (MIT)

• Michigan

  • Jens Schmidt (Michigan State University)

  • Nils Walter (University of Michigan, Ann Arbor)

  • Julie Biteen (University of Michigan, Ann Arbor)

  • Matthew Comstock (Michigan State University)

• Minnesota

  • Elias Puchner (University of Minnesota, Twin Cities)

• Missouri

  • Shizhen Wang (University of Missouri, Kansas City)

  • Timothy Lohman (Washington University, Saint Louis)

  • Andrea Soranno (Washington University, Saint Louis)

  • Eric Galbert (Washington University, Saint Louis)

  • Roberto Galletto (Washington University, Saint Louis)

  • Edwin Anthony (Saint Louis University)

  • Gavn King (University of Missouri, Columbia)

  • Maria Mills (University of Missouri, Columbia)

  • Matthew Lew (Washington University, Saint Louis)

• Nebraska

  • Piero Bianco (University of Nebraska Medical Center)

  • Qing Tang (University of Nebraska, Lincoln)

• New Jersey

  • Sang-Hyuk Lee (Rutgers University)

  • Keith Mickolajczyk (Rutgers University)

  • Haw Yang (Princeton University)

  • Thomas Gregor (Princeton University)

• New Mexico

  • Diane Lidke (University of New Mexico)

• New York

  • Ruben Gonzalez Jr. (Columbia University)

  • Eric Greene (Columbia University)

  • Alexandros Pertsinidis (Memorial Sloan Kettering Cancer Center)

  • Shixin Liu (Rockefeller University)

  • Robert Singer (Albert Einstein College of Medicine)

  • Arne Gennerich (Albert Einstein College of Medicine)

  • Michelle Wang (Cornell University)

  • Eli Rothenberg (New York University)

  • Arnd Pralle (University at Buffalo)

  • Dmitri Ermolenko (University of Rochester)

  • Mark Bown (Stony Brook University)

  • Peng Chen (Cornell University)

  • Simon Scheuring (Weill Cornell Medicine)

  • Elio Abbondanzieri (University of Rochester)

• North Carolina

  • Keith Weninger (North Carolina State University)

  • Sharonda LeBlanc (North Carolina State University)

  • Kevin Welsher (Duke University)

  • Christoph Schmidt (Duke University)

  • Christine Payne (Due University)

• Ohio

  • Hamza Balci (Kent State University)

  • Michael Poirier (Ohio State University)

  • Comert Kural (Ohio State University)

  • Xuefeng Wang (University of Cincinnati)

  • Hanbin Mao (Kent State University)

  • Jiajie Diao (University of Cincinnati)

  • H. Peter Lu (Bowling Green State University)

• Oregon

  • Thuy Ngo (Oregon Health Science University)

• Pennsylvania

  • Melike Lakadamyali (University of Pennsylvania)

  • Ruobo Zhou (Pennsylvania State University)

  • Elizabeth Rhoades (University of Pennsylvania)

  • Mustafa Mir (Children's Hospital of Philadelphia)

  • William Hancock (Pennsylvania State University)

  • Tae-Hee Lee (Pennsylvania State University)

  • Bennet van Houten (University of Pittsburg)

• South Carolina

  • Laura Finzi (Clemson University)

• Tennessee

  • Scott Blanchard (St. Jude's Children's Hospital)

  • Rajan Lamichhane (University of Tennessee, Knoxville)

  • Matthew Lang (Vanderbilt University)

  • Gregor Neuert (Vanderbilt University)

• Texas

  • Ilya Finkelstein (University of Texas, Austin)

  • Lanying Zeng (Texas A & M University)

  • Siegfried Musser (Texas A & M University)

  • Hyeongjun Kim (University of Texas, Rio Grande Valley)

• Vermont

  • David Warshaw (University of Vermont)

• Virginia

  • Andreas Gahlmann (University of Virginia)

• Washington

  • Charles Asbury (University of Washington, Seattle)

  • Jens Gundlach (University of Washington, Seattle)

• Wisconsin

  • Aaron Hoskins (University of Wisconsin, Madison)

  • Randall Goldsmith (University of Wisconsin, Madison)