2003-2011 Ph.D. University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina
Department: Cell and Developmental Biology
Title: The role of Yes-Associated Protein (YAP) in vertebrate development.
Mentor: Sharon L. Milgram, Ph.D.
Other Mentors: Sally A. Moody, Ph.D. (George Washington University)
Kenneth L. Kramer, Ph.D. (NHLBI-NIH, now Creighton University)
Frank Conlon, Ph.D. (University of North Carolina at Chapel Hill)
Doctoral Hooding Ceremony video & University of the people video
1999-2003 M.S. Medical University of South Carolina (MUSC), Charleston, South Carolina
Department: Molecular and Cellular Biology and Pathobiology
Title: Structure/Function studies of Pigment-Epithelium Derived Factor (PEDF).
Mentor: Jian-xing Ma, M.D., Ph.D.
1993-1997 B.S. University of South Carolina, Columbia, Columbia, South Carolina
2022-current Visiting Assistant Professor, Department of Biology, Worcester State University
Taught the following courses:
The Human Animal (BI-103) & Lab (BI-104), primarily designed to serve the Communication Sciences & Disorders (CSD) Majors, but also served as a general education science course & lab for science non-majors.
Introduction to Cellular & Molecular Biology & Lab (BI-141), a second-semester introductory biology course & lab for biology & biotechnology majors.
Anatomy and Physiology I (BI-161) & II (BI-162) & Lab, a two-semester A&P course & lab for students in Nursing, Occupational Therapy, Biology (pre-Physical Therapy, pre-Physician Assistant, pre-Pharmacy students), & Public Health majors.
Used Visible Body Courseware; microscopes; tissue slides; cow eye, sheep heart, & cow kidney dissections; student-recorded electrocardiograms (ECG); spirometers; and a variety of quality models. Lab partners were provided lists of the anatomical features they would need to identify during each scheduled lab practical. Students then worked together to practice quizzing each other. Once both students felt confident, I circled the classroom and quizzed them to ensure they could identify each feature before leaving the lab for the day.
Met with A&P faculty to discuss ways to improve the courses and measure student assessment.
Used Blackboard Ultra to provide learning materials, chapter homework assignments, formative assessments, feedback, and grades.
Served on the departmental Assessment Committee. I also met with two different groups of faculty (Intro Cell/Molec Bio & Human Bio/A&P)) to help create questions for the newly implemented departmental assessment exam given to freshmen and seniors.
Advised Biology major students in their course selections each semester and talked with them about their career goals and the additional steps they could take to help achieve them.
2021-2022 Visiting Assistant Professor, Department of Biology, Coe College
Taught the following courses:
Human Biology (BIO-100), a general education science course for non-majors.
Human Biology Lab (BIO-100L), a general education science lab course for non-majors.
Cellular & Molecular Biology (BIO-145), a first-semester introductory biology course for science majors and pre-nursing students.
Cellular & Molecular Biology Lab (BIO-145L), a first-semester biology lab for science majors
Organismal & Ecological Biology (BIO-155), a second-semester introductory biology course for science majors.
Organismal & Ecological Biology Lab (BIO-155L), second- semester introductory biology course for science majors.
Integrated Human Anatomy (BIO-315), a senior-level elective course consisting mostly of pre-professional school students.
Used the Gray’s Anatomy for Students textbook for this course because these senior-level pre-professional school students had already been exposed to human anatomy in the freshman Organismal & Ecological Biology course/lab as well as in an Integrated Human Physiology (BIO-375) course. Therefore, we were able to go a bit more in-depth to best prepare them for their post-graduate career.
Integrated Human Anatomy Lab (BIO-315L), a senior-level elective lab consisting mostly of pre-professional school students.
Used a SynDaver, cat dissection, a variety of quality models, human bones, plastinated human organs, practice labeling worksheets, and additional question-based worksheets that helped students review material from the lecture course.
Used Moodle to provide learning materials, weekly homework assignments, weekly muddiest point assignments, formative assessments, active learning activities, feedback, summative assessments, and grades.
2020-2021 Assistant Professor, Department of Biology/Chemistry, Springfield College
Taught the following courses:
Anatomy & Physiology Concepts I (BIOL-130), 2 sections, first semester of an A&P course for science non-majors (i.e. Athletic Trainers, Exercise Science, Physical Education, Occupational Therapy, Sports Biology, Health Science etc.).
Anatomy & Physiology Concepts II (BIOL-131), 2 sections, second semester of an A&P course for science non-majors (i.e. Athletic Trainers, Exercise Science, Physical Education, Occupational Therapy, Sports Biology, Health Science etc.).
Bioscience I (BIOL-121), a first-semester introductory biology course for science majors.
Bioscience II (BIOL-122), a second-semester introductory biology course for science majors.
Bioscience I (BIOL-123), 2 sections, a first-semester biology labs for science majors.
Developmental Biology (BIOL-341), a senior-level elective course for science majors.
Used Brightspace (D2L) to provide learning materials, weekly homework assignments, weekly muddiest point assignments, formative assessments, active learning activities, feedback, summative assessments, and grades.
Used Zoom to provide synchronized classroom content, which included the utilization of breakout rooms for completion of group in-class active learning activities.
2019-2020 Visiting Assistant Professor, Department of Biological and Health Sciences, University of Pittsburgh at Bradford
Taught the following courses:
Human Biology (BIOL-0112), an introductory A&P course for nursing students and a general education science course for non-majors.
Incorporated a group critical-thinking case study final project into the course.
Held Sunday evening, outside of class, review sessions for each of the 6 course quizzes.
Molecular Biology (BIOL-1402), an upper-level course for junior and senior biology majors.
Used Blackboard (Bb) modules to provide learning materials and formative assessments, receive required work, and provide grades. Practiced using Canvas, which is set to replace Bb for the upcoming year.
Learning materials focused on reviewing and extending information covered in the intro course, while also learning about the techniques they might use if they want to work in a lab after graduation.
Incorporated the reading, formative assessment, and in-class discussions of current scientific literature.
Molecular Biology Lab (BIOL-1412), an upper-level lab course for junior and senior biology majors.
Organized a series of available EDVOTEK labs to coincide with the material covered in the lecture course.
Responsible for the entire setup and breakdown of these lab experiences.
Genomics, Proteomics, & Bioinformatics (BIOL-1410), an upper-level course for junior & senior bio majors.
Short introductory lectures were used to review over material from previous classes and introduce new daily material for use in the daily in-class computer activity.
In-class computer activities allowed me to walk around the classroom and help individual students who were having trouble or had specific questions. My goal for the computer activities were to provide the students an opportunity to build real-world skills by using computer-based software that they might encounter or need to use if they work in a research lab following graduation.
Introduction to Cell and Molecular Biology w/ labs (BIOL-0101) using a free online textbook (OpenStax).
Weekly online multiple-choice homework questions and in-class quizzes provided students with plenty of practice with the material.
Lectures were divided into three sections, which included embedded questions after each section to allow students to see how well they understood the covered material. For difficult material, I would go back over the topics again during the lecture and in lab, so that they would get as many repetitions as possible.
Recorded lectures using Panopto after transitioning to online learning during the COVID-19 shut down.
Practiced using Canvas, which was set to replace Bb for the upcoming year.
Capstone: Biology (BIOL-1451), a two-semester comprehensive course for senior biology majors whereby they come up with their own research project based on topics of specific interest to them. Students may choose to do a hands-on research project in the lab or a literature-based research project. They collect primary literature references, write an annotated bibliography, develop a project outline, write a 20-page paper on their research project, and present their work as a poster or presentation.
Set up both a freshwater aquarium for a project using snails (Pomacea bridgesii and Pomacea canaliculata) and a chilled, saltwater aquarium for two projects using purple sea urchins (Strongylocentrotus purpuratus). I used my own funds for these endeavors, so the equipment for these projects can come with me.
2018-2019 Assistant Professor, Department of Biological Sciences, Duquesne University
Taught the following courses:
Advanced General Biology (BIOL-115), a freshman-only introductory course for biology majors.
Provided in-class active learning activities, such as Think-Pair-Shares and case studies to enhance learning.
Used Nearpod, an online slideshow lecture tool, to deliver supplemental instruction outside of the classroom.
Assessed student learning of online learning materials using timed online quizzes.
Used Blackboard (Bb) modules to provide learning materials, receive required work, and provide grades.
Anatomy and Physiology I and II labs (BIOL-208, 209)
Developmental Biology (BIOL- 313)
Compared and contrasted the development of vertebrate model organisms and humans.
Used a variety of formative and summative assessments to evaluate student learning.
Biology Research Forum (BIOL-394), a course designed for students performing research with a faculty mentor. The course guided students in writing their senior thesis and developing an oral presentation based on their research project. Students also read and critically assessed scientific literature, discussed the ethics of research and publication, and critiqued the work of their peers.
Seminar course (BIOL- 490/690) for junior and senior biology majors and graduate students.
Undergraduate and graduate students attended and sometimes participated in weekly departmental research presentations by biological scientists. Students provided written reports illustrating their presence.
Participated in the following 9-week asynchronous online faculty certification course:
"Foundations in Online Teaching & Learning"
Developed an online course for Anatomy & Physiology I.
Attended the following events, which were sponsored by the Center for Teaching Excellence:
Writing Prompts that Work: A Workshop in Crafting Effective Assignment Sheets for Writing Projects
Small Changes Advancing LEarning (SCALE) Micro-Workshop: Be the Spark
Attended the Diversity and Inclusion Speaker Series.
Used Blackboard (Bb) modules to provide learning materials, receive required work, and provide grades.
2017-2018 Instructor, Departments of Biology and Kinesiology, Coe College
Taught the following courses:
Physiology of Exercise (PE-525), a capstone course for Kinesiology seniors.
Created daily worksheets, which encouraged students to read the book and come to class prepared and ready to discuss what they learned from their reading and the completed worksheet.
Used Moodle to provide and receive required worksheets, chapter quizzes, and grades.
Incorporated four physiology labs, using iWorx equipment and software, into the course for the first time.
Introduction to Biology Laboratory (BIO-140), two sections, the lab associated with the department’s introductory cell and molecular biology course, typically taken exclusively by freshmen.
Organismal and Ecological Biology Laboratory (BIO-150), which focuses on fetal pig and crayfish dissections, invertebrate behavior, respiration rates of African hissing cockroaches, and field ecology.
Both labs, BIO-140 and BIO-150, are writing emphasis courses, which require a major lab report to be submitted and feedback from the professor to be provided for student revision and improvement of the original draft.
Volunteered to help the Writing Committee judge a set of written portfolios submitted by the First Year Seminar (FYS) Program faculty for the Outstanding FYS Portfolio Prize.
2017 Adjunct Science Faculty, Kirkwood Community College
Taught two sections of Nutrition (BIO-151).
Created daily worksheets, which encouraged students to read the book and come to class prepared and ready to discuss what they learned from their reading and the completed worksheet.
Met with English as a second language (ESL) students for an additional half hour after each class to provide supplemental instruction.
Used Talon to provide and receive required daily worksheets and chapter quizzes.
2016 Tutor III, Community College of Baltimore County (CCBC)
Tutored CCBC students in Biology, Nutrition, Human Anatomy/Physiology, and Microbiology.
2014 (Spring) Judge for the Northwest Association for Biomedical Research (NWABR)
Middle School Essay Contest entitled: “Biomedical Breakthroughs and my life”
2013-2014 Laboratory mentor to Notre Dame of Maryland University
Undergraduate Honors Student
2013 (Fall) Teaching Fellows Program for Johns Hopkins Medical Institute Postdoctoral Fellows at Notre Dame of Maryland University
Trained in didactic teaching under the direction of Janice Bonner, Ph.D. at Notre Dame of Maryland University
Recruited and mentored a Notre Dame of Maryland University Honors student on a lab project.
2013 (Spring) Judge for the Northwest Association for Biomedical Research (NWABR)
Middle School Essay Contest entitled: “Biomedical Breakthroughs and my life”
2007-2009 Laboratory mentor, 2 NIH Post-Baccalaureate Fellows
2006-2007 Laboratory mentor to UNC Biology Undergraduate Honors Student
Honors Thesis: Identifying the transcriptional regulatory elements of Yes-associated protein (YAP).
2005 Laboratory mentor to UNC Biology Undergraduate Honors Student
Honors Thesis: Developmental expression of YAP and TAZ in Xenopus laevis.
2013-2014 Postdoctoral Fellow
Research Summary: Performed experiments to better understand the etiology of glaucoma, a major cause of irreversible blindness worldwide. Patients with glaucoma typically exhibit increased intraocular pressures, which ultimately leads to optic nerve degeneration through progressive retinal ganglion cell (RGC) loss. Because glaucoma is most prevalent in the aged and there are few genetic mutations associated with the disease, environmental factors and epigenetic modifications likely play a critical role in the pathology of the disease. Therefore, I worked to identify and validate the epigenetic changes (ie. DNA methylation) associated with glaucomatous damaged retinas.
Worked to identify and validate new biomarkers of primary open-angle glaucoma (POAG) and age-related macular degeneration (AMD) using Illumina 450K methylation arrays and determining gene expression changes in blood and retinal samples from human patients.
Cloned, characterized, and validated the existence of an uncharacterized, hypothetical protein.
Worked to identify whether DNA methylation is responsible for the retinal exclusivity of a certain neuronal splice variant.
Maintained mouse colony, which included the regular genotyping and breeding of 40-60 cages of mice consisting of 10-16 different genotypes.
Established the isolation of primary retinal ganglion and Muller cells in the lab by compiling 4 protocols into one succinct protocol, which was then provided to other labs at the Wilmer Eye Institute and around the country.
Tested whether reductions in DNA methylation extend retinal ganglion cell survival using single, double, and triple: (Dnmt1, Dnmt3a, Dnmt3b)2lox/2lox, Cre-inducible knockout mice.
Used Pyromark Q24 sequencer and software to design PCR primer pairs and sequencing primers to determine the genomic DNA methylation status of individual sites and loci.
Used lipid-mediated transfection, immunofluorescent staining, and confocal microscopy of cultured cell lines to determine the localization of an uncharacterized, hypothetical protein
Participated in Dean’s Research Integrity Lecture Series at The Johns Hopkins University School of Medicine.
2011-2013 Postdoctoral Fellow
Research Summary 1: Performed experiments to uncover the key molecular pathways involved in regenerating pancreatic β-cells from cells already present in the pancreas. Zebrafish, unlike mice, possess a profound ability to efficiently regenerate tissues and/or organs following chemical, genetic, or physical-mediated injury. Understanding the key developmental roles each of these molecular pathways play in pancreatic organ formation yields essential information for understanding how to best regenerate pancreatic β-cells from existing pancreatic progenitors.
Identified specific markers of a putative adult pancreatic β-cell progenitor cell using cell sorting of transgenic zebrafish pancreata and subsequent RNAseq analysis of single and paired-end reads.
Verified RNAseq analyses and the validity of these genes as cell-specific markers using qPCR, BAC transgenesis, immunofluorescence of mouse and zebrafish tissue sections, and confocal microscopy.
Implemented the recently developed Q transcriptional regulatory system for zebrafish transgenesis.
Created Cre-inducible zebrafish transgenic lines for the purpose of inhibiting or overactivating Notch signaling in a cell type specific manner.
Used TALENs (transcription activator-like effector nucleases) to create gene-specific mutant zebrafish.
Critically reviewed manuscripts and presented my work at international meetings.
2007-2011 Graduate student in the NIH Intramural Research Training Program, (Symposium)
National Heart, Lung, and Blood Institute, National Institutes of Health (NIH), Bethesda, Maryland
Mentor: Sharon L. Milgram, Ph.D.
2003-2007 Graduate Student in the Department of Cell and Developmental Biology
University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina
Mentor: Sharon L. Milgram, Ph.D.
Research Summary: Yes-associated protein 65 (YAP) functions as both a transcriptional co-activator and as a scaffolding protein. Given that YAP knockout mice struggled to progress normally through early development, in part because of nutritional deficiencies, I sought to better characterize a role for YAP by using embryos that develop externally: Xenopus laevis and Danio rerio. YAP morpholino (MO)-mediated loss-of-function resulted in a delay of mesoderm induction and severely impaired A-P axis elongation, phenotypes that were similar to YAP-/- mice. YAP gain-of-function experiments in Xenopus laevis expanded the progenitor populations in the neural plate and neural plate border zone, while concomitantly inhibiting differentiation markers for the neural crest, preplacodal ectoderm, hatching gland, epidermis, and somitic muscle.
Investigated the role of Yes-associated protein (YAP) in vertebrate development by designing and performing experiments in Xenopus laevis and Danio rerio using microinjections of morpholinos (MOs) and in vitro transcribed RNAs, in situ hybridization, luciferase and β-galactosidase activity assays, and chromatin immunoprecipitations.
Evaluated transcriptional control of YAP using luciferase and β-galactosidase activity assays and gel-shift analysis.
Created wild type, yap+/-, and yap-/-mouse embryonic fibroblast (MEF) cell lines using viral-mediated immortalization.
Determined gene expression changes in wild type and yap-/-mouse embryonic fibroblast (MEF) cell lines using microarray analyses and RT-PCR.
Evaluated putative YAP transcriptional targets in the established MEF cell lines by adding YAP back to the null cells via nucleofection.
Evaluated protein-protein interactions with YAP using radioactive in vitro binding assays, GST pulldowns, and co-immunoprecipitations.
Used the following techniques: whole mount in situ hybridization of Xenopus laevis embryos, in vitro transcription, electron microscopy, time-lapse video microscopy, western blot analyses (enhanced chemiluminescence (ECL) and fluorescent detection using a Li-Cor Odyssey), qPCR, PCR, RT-PCR, Amaxa nucleofector technology, GST-pulldowns using in vitro translated proteins using [35S] radiolabeled methionine or purified bacterial-induced proteins, baculovirus-mediated protein expression and subsequent purification, co-immunoprecipitations, retroviral immortalization of primary mouse embryonic fibroblasts, microarray analyses, electrophoretic mobility shift assays (EMSA), in vitro microinjections (in vitro transcribed RNAs, morpholinos, and DNA), chromatin immunoprecipitation (ChIP), luciferase assays, cell fractionation, and whole mount immunostaining of Xenopus laevis embryos for these investigations.
Participated in a semester long Grant Writing course in which we critiqued both funded and nonfunded grants from a variety of investigators.
Attended a weeklong scientific writing workshop given by George Gopen, J.D., Ph.D and entitled “Writing from the reader’s perspective”
Critically reviewed manuscripts and presented my work at international meetings.
Mentored two UNC Honors Biology undergraduates through their Honors theses. (see above)
Mentored two postbaccalaureates during my time at the NIH.
1999-2003 Graduate student in Molecular and Cellular Biology and Pathobiology
Medical University of South Carolina, Charleston, South Carolina
Mentor: Jian-xing Ma, M.D., Ph.D.
Research Summary: Pigment-Epithelium Derived growth Factor (PEDF) is an established neurotrophic and anti-angiogenic factor. I evaluated the transcriptional control of PEDF in a retinal pigment epithelial cell line using β-galactosidase/luciferase assays and gel-shift analyses. Investigated the anti-angiogenic abilities of PEDF on the retinal microvasculature by isolating primary human and bovine retinal microvascular cells (endothelial and pericytes), purifying wild type and deleted forms of PEDF from E. coli, and adding the purified proteins to the cultured cells in order to determine the region of PEDF responsible for its anti-angiogenic properties.
Evaluated the transcriptional control of Pigment-Epithelium Derived growth Factor (PEDF) in a retinal pigment epithelial cell line (D407) using β-galactosidase/luciferase assays and electrophoretic mobility shift assays (EMSA).
Purified wild type and deleted forms of PEDF using E. coli induced His-tagged proteins and nickel columns.
Isolated primary human and bovine retinal microvascular cells (endothelial and pericytes) and add the purified proteins to the cells in order to determine the region of PEDF responsible for its anti-angiogenic properties.
Used ELISA to measured VEGF levels in cell-conditioned media.
1997-1999 Research Assistant in the Department of Pathology/Ophthalmology
University of South Carolina-School of Medicine, Columbia, South Carolina
Mentors: Ramesh Tripathi, M.D., Ph.D. & Brenda T. Tripathi, Ph.D.
Research Summary: Performed experiments to better understand the etiology of glaucoma, a major cause of irreversible blindness worldwide. Patients with glaucoma typically exhibit increased intraocular pressures due to obstruction or greater resistance in the aqueous outflow facility. This increased outflow resistance is due to loss of trabecular meshwork cells resulting in increased extracellular matrix deposition and alterations in local growth factors. Although some patients respond well to pharmacological treatments to lower their intraocular pressure, some do not and require trabeculectomy (filtration surgery). Unfortunately, this surgery sometimes fails due to the overproliferation of Tenon's capsule fibroblasts which attempt to seal the filtering incision. Therefore, I performed experiments to analyze their proliferation rates and extracellular deposition in the presence of defined growth factor cocktails.
Organized and maintained the lab to maximize lab productivity, which included preparing orders for scientific sourcing and procurement.
Performed a number of experiments involving the primary isolation and culturing of porcine trabecular meshwork cells and Human Tenon’s capsule fibroblasts.
Critically reviewed manuscripts and presented my work at an international Ophthalmology meeting (ARVO).
1996 (Summer) Howard Hughes Summer Research Fellow
University of South Carolina, Columbia
Mentor: Robert P. Lawther, Ph.D.
2014 National Eye Institute fellowship program entitled, "Fundamental Issues in Vision Research", at the Marine Biological Laboratory (MBL);
August 10-23, 2014.
2001 Fight for Sight (Prevent Blindness America) Student Fellowship
“Transcriptional Regulation of PEDF under Hypoxic Conditions.”
2001 (March) Sigma Xi, Grant-in-Aid Award via National Academy of Sciences
“Transcriptional Regulation of PEDF under Hypoxic Conditions.”
2000 Sigma Xi, Grant-in-Aid Award via National Academy of Sciences
“Gene Regulation of Retinal Capillary Cells under Normal and Glycemic Conditions.”
2013-2014 Johns Hopkins Postdoctoral Association (JHPDA)
Chair, Policy and Advocacy (P&A) Committee
Postdoctoral Representative at monthly Johns Hopkins Faculty Senate meetings
Postdoctoral Representative at monthly Student Health Committee meetings
Led monthly P&A Committee Meetings by setting the meeting’s agenda and disseminating the minutes of the meetings to members and JHPDA executive board members.
Successfully advocated for the enforcement of equal pay of ALL postdoctoral fellows based on the policy set forth by the university that ALL fellows are to be paid according to the established National Research Service Award (NRSA) guidelines resulting in increased pay for fellows.
Developed, administered, and summarized results from the yearly JHPDA P&A survey. Survey participation doubled from previous years due to increased online presence and campus-wide outreach.
Organized and led P&A event entitled, “Speak out for science!”. Led 20 postdoctoral fellows and graduate students to Washington D.C. and met with the offices of 5 U.S. Congressional Representatives to advocate for increased medical research funding.
Attended and reported on P&A committee progress/events at monthly JHPDA meetings.
Member, Communications Committee
Crafted and emailed weekly JHPDA bulletins on a rotating basis to ~1300 fellows.
Updated the JHPDA website with new events and information, as needed.
Helped put on annual JHPDA sponsored events, such as the Postdoc Research Symposium, POSTDOCtorfest, the December Holiday Party, and the first annual Hopkins Postdoc Retreat.
Elected Co-President of the JHPDA for the upcoming year.
Peer-reviewed
F. Delaspre, R.L. Beer, M. Rovira, W. Huang, G. Wang, S. Gee, M.D. Vitery, S.J. Wheelan, M.J. Parsons. Centroacinar cells are progenitors that contribute to endocrine pancreas regeneration. Available online 7 July, 2015 Diabetes (PMID:26153247), (pdf)
A. Subedi, M. Macurak, S.T. Gee, E. Monge, M.G. Goll, C. Potter, M.J. Parsons, M.E. Halpern. Adoption of the Q transcriptional regulatory system for zebrafish transgenesis. Available online 20 June, 2013 Methods http://dx.doi.org/10.1016/j.ymeth.2013.06.012. PMID: 23792917, (pdf)
S.T. Gee, S.L. Milgram, K. Kramer, F.L. Conlon, S.A. Moody. Yes-associated protein 65 (YAP) expands neural progenitors and regulates pax3 expression in the neural plate border zone. Available online 8 June, 2011 PLoS ONE 6(6):e20309. doi:10.1371/journal.pone.0020309. (PMID: 21687713), (pdf)
E.K. Vidro, S. Gee, R. Unda, J.X. Ma, A. Tsin. Glucose and TGF-β2 modulate the viability of cultured human retinal pericytes and their VEGF release. Current Eye Research 33(11):984-993, 2008. (PMID: 19085381), (pdf)
J. Tombran-Tink, N. Lara, S.E. Apricio, P. Potluri, S. Gee, J-x. Ma, G. Chader and C. J. Barnstable. Retinoic Acid and dexamethasone regulate the expression of PEDF in retinal and endothelial cells. Experimental Eye Research 78(5):945-955, 2004. (PMID: 15051476), (pdf)
G. Gao, Y. Li, S. Gee, A. Dudley, J. Fant, C. Crosson, J-x. Ma. Down-regulation of VEGF and up-regulation of PEDF: A possible mechanism for the anti-angiogenic activity of plasminogen Kringle 5. J Biol Chem 277(11):9492-7, 2002. (PMID: 11782462), (pdf)
G. Gao, Y. Li, D. Zhang, S. Gee, C. Crosson, J-x. Ma. Unbalanced expression of VEGF and PEDF in ischemia-induced retinal neovascularization. FEBS Letters. 489(2-3):270-276, 2001. (PMID: 11165263), (pdf)
Lay Media
Stephen Gee. Steroids and Glaucoma. Summer 1999 Prevent Blindness News. (pdf)
Stephen Gee. The AGEing (Advanced Glycation End products) Process. Diabetes Forecast. 51(10):72-74, 1998. (pdf)
A. Subedi, M. Macurak, S. Gee, M. Goll, C.J. Potter, M.J. Parsons, M.E. Halpern. Application of a new transcriptional regulatory system to zebrafish. 10th International Zebrafish Genetics and Development, Madison, Wisconsin, June 20-24, 2012.
S.T. Gee, F. Delaspre, W. Huang, M.J. Parsons. The role of the Notch-signaling pathway in pancreas regeneration. American Diabetes Association 72nd Scientific Sessions, Philadelphia, Pennsylvania, June 8-12, 2012.
S.T. Gee, S.L Milgram, K.L. Kramer, F.L. Conlon, S.A. Moody. YAP is an important regulator of cell differentiation. Cold Spring Harbor Meeting on Vertebrate Organogenesis, Cold Spring Harbor, New York, April 27-May 1, 2010.
S.T. Gee, S.L. Milgram, K.L. Kramer, F.L. Conlon, S.A. Moody. YAP is an important regulator of cellular differentiation. Society for Developmental Biology 68th Annual Meeting, San Francisco, California, July 23-27, 2009.
S.T. Gee, S.L. Milgram, K.L. Kramer, F.L. Conlon, S.A. Moody. Yes-associated protein 65 (YAP) expands neural and somitic progenitors and regulates Pax3 expression in the paraxial mesoderm. Society for Developmental Biology Mid-Atlantic Regional Meeting, College Park, Maryland, May 15-16, 2009.
S.T. Gee, F.L. Conlon, S.L. Milgram. Yes-associated protein 65 (YAP65) is required for early Xenopus development. Society for Developmental Biology 65th Annual Meeting, Ann Arbor, Michigan, June 17-21, 2006. Developmental Biology 295:393, 2006.
J. Tombran-Tink, S. Gee, N. Lara, J-x Ma, C.J. Barnstable. PEDF expression and promoter activity is regulated by retinoic acid. ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 44:E-5236, 2003.
J-x Ma, G. Gao, Y. Li, S. Gee, J. Fant, C.E. Crosson, A. Dudley. Angiogenic inhibitor K5 inhibits retinal neovascularization possibly through down-regulation of VEGF and up-regulation of PEDF. ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 43:E-1279, 2002.
S.T. Gee, J. Tombran-Tink, J-x. Ma. Identification of a putative enhancer region for Pigment Epithelium-Derived Factor (PEDF). ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 43:E-689, 2002.
G. Gao, Y. Li, S. Gee, P.I. Kaufman, R.A. Saunders, C. Crosson, J-x. Ma. Unbalanced retinal VEGF and PEDF expression in oxygen-induced retinopathy. ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 42(4):S92, 2001.
S.M. Hammad, Y. Bellil, S. Gee, T.J. Lyons. Glycoxidized LDL induces apoptosis in retinal capillary cells. 17th International Diabetes Federation Congress. Mexico City, Mexico. November 5-10, 2000.
Stephen T. Gee, Junping Li, Brenda J. Tripathi, Ramesh C. Tripathi. Effects of TGF-beta 1, TGF-beta 2, and TGF-beta 3 on human Tenon's capsule fibroblast proliferation. ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 40(4):S662, 1999.
B.J. Tripathi, C.E. Yockey, S. Gee, P.C. Tripathi, R.C. Tripathi. Metabolism and growth of trabecular cells are impaired on collagenVI substrate. ARVO Annual Meeting, Investigative Ophthalmology and Visual Science. 39(3):S253, 1998.
2000-2015 I served as a Volunteer Editor for Investigative Ophthalmology and Visual Science (IOVS). I am acknowledged for my IOVS editorial work in the following publications:
Manuel F. Bande, Maria Santiago, Maria Jose Blanco, Purificacion Mera, Carmela Capeans, Maria Xose Rodrigquez-Alvarez, Maria Pardo, and Antonio Pineiro. Serum DJ-1/PARK7 is a potential biomarker of choroidal nevi transformation. IOVS 53(1):62-67, 2012. (pdf)
Mio Oshikawa, Chihiro Tsutsui, Tomoko Ikegami, Yuki Fuchida, Maki Matsubara, Shigeru Toyama, Ron Usami, Kuniyo Ohtoko, and Seishi Kato. Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method. IOVS 52(9):6662-6670, 2011. (pdf)
Stefan Arnhold, Helmut Klein, Irina Semkova, Klaus Addicks, and Ulrich Schraermeyer. Neurally selected embryonic stem cells induce tumor formation after long-term survival following engraftment into the subretinal space. IOVS 45(12):4521-4255, 2004. (pdf)
Louis Tong, Seang-Mei Saw, Jyh-Kuen Siak, Gus Gazzard, and Donald Tan. Corneal thickness determination and correlates in Singaporean schoolchildren. IOVS 45(11):4004-4009, 2004. (pdf)
Kenichi Kimoto, Kazuo Nakatsuka, Noritaka Matsuo, and Hidekatsu Yoshioka. p38 MAPK mediates the expression of type I collagen induced by TGF-β2 in human retinal pigment epithelial cells ARPE-19. IOVS 45(7):2431-2437, 2004. (pdf)
Zhiqiang Pan, Yu Chen, Wenhua Zhang, Ying Jie, Na Li, and Yuying Wu. Rat corneal allograft survival prolonged by the superantigen staphylococcal enterotoxin B. IOVS 44(8):3346-3351, 2003. (pdf)
Yuichi Kaji, Shiro Amano, Tomohiko Usui, Tetsuro Oshika, Kenji Yamashiro, Susumu Ishida, Kaori Suzuki, Sumiyoshi Tanaka, Anthony P. Adamis, Ryoji Nagai, and Seiko Horiuchi. Expression and function of receptors for Advanced Glycation End-products in bovine corneal endothelial cells. IOVS 44(2):521-528, 2003. (pdf)
Yoko Ogawa, Kazuto Yamazaki, Masataka Kuwana, Yukihiko Mashima, Yu Nakamura, Susumu Ishida, Ikuko Toda, Yoshihisa Oguchi, Kazuo Tsubota, Shinichiro Okamoto, and Yutaka Kawakami. A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD. IOVS 42(1):111-119, 2001. (pdf)