Normally, when you do RNA FISH, you need to do an overnight hybridization. Using the standard protocol, you can reduce this to around 4-10 hours or so, but that's about as fast as it goes. Sydney Shaffer (graduate student in the lab), however, has introduced a modified protocol in which you can do RNA FISH hybridizations in just 5 minutes, sometimes as fast as 30 seconds! The key is to use an alcohol fixative rather than a cross-linking fixative while increase probe concentration. And then... magic. Also, while you do have to use a higher concentration of probe, you also use a smaller volume, so you don't even burn that much extra material. We have just published the protocol in PLoS ONE, and below is a nice and detailed protocol.
Also, here's a video:
Prepare wash buffer and hybridization buffer (same as usual protocol):
Wash buffer: 2X SSC with 10% formamide
Hybridization buffer: 2X SSC with 10% dextran sulfate and 10% formamide
Turbo hybridization: