Submissions

Submitting a Library

To get in the queue:

• Submit sequencing requests and access the submission form through iLabs.

• Save the form using your sample name (your initials + PI initials + 2-digit number).

  • Examples: EDJD01, MJPW02-p

  • Do not use any extra punctuation, spaces, etc. This makes it easier to read the tube labels.

  • Write the date on the side of the tube.

• Put 20ul of your sample in a 1.5ml low bind tube at 5-10nM (this is around 1.5-3ng/ul). For each additional lane, please add an additional 10ul of library.

• Label the top of the tube with the sample name and the side of the tube with the date.

• Drop off the sample tube to the CAT, and custom primer if needed, in the small freezer on the ground outside the double doors. This is near room S271.

• Custom primers: Dropoff 10ul of a 100uM aliquot in a 1.5ml tube. Label it with your sample name + "Primer"

• Shipping samples:

  • Place 1.5ml tubes in a secondary container like a 50ml conical with kimwipes prevent samples from jostling.

  • Ship via UPS or FedEx to:

    • UCSF CAT Lab GH-S252

    • 600 16th Street

    • San Francisco CA 94158

When submitting manuscripts, please acknowledge the CAT by including the text: “Sequencing was performed at the UCSF CAT, supported by UCSF PBBR, RRP IMIA, and NIH 1S10OD028511-01 grants.”