Main research interest:
My main research interest is to understand how AAA+ ATPases are using ATP hydrolysis to remodel their specific substrates. Indeed, in all kingdom of life, a same structural unit (AAA+ core) is used for very versatile cellular activities (for example for transcription activation, protein degradation, or microtubule severing).
Understanding the conserved feature of such domain is critical to understand how these different molecular machines are working. In addition, more and more human diseases have been linked to defective proteins member of the AAA+ protein family. Strikingly, deep sequencing approaches have reported that the mutations responsible for the activity default were, in most of the time, located into the AAA+ core, pointing out the importance of understanding the molecular bases allowing the AAA+ core to use ATP hydrolysis and to transmit this "energy" to the remodeling motifs.
Currently, I am using the AAA+ microtubule-severing enzyme Katanin as system model.
Contact : email
Recent papers:
Sanial M, Miled R, Alves M, Claret S, Joly N, Proux-Gillardeaux V, Plessis A, Léon S.
Biol Cell. 2025 Feb;117(2):e2400161. doi: 10.1111/boc.202400161. PMID: 39924827
Enhanced Golden Gate Assembly: evaluating overhang strength for improved ligation efficiency
Patryk Strzelecki*, Nicolas Joly*, Pascal Hébraud, Elise Hoffmann, Grzegorz M Cech, Anna Kloska, Florent Busi, Wilfried Grange. (*co-first author)
Nucleic Acids Research, 2024; https://doi-org.insb.bib.cnrs.fr/10.1093/nar/gkae809
Beaumale E, Van Hove L, Pintard L, Joly N.
J Cell Biol. 2024 Apr 1;223(4):e202308023. doi: 10.1083/jcb.202308023. Epub 2024 Feb 8. PMID: 38329452
Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Joly N, Pintard L.
Elife. 2022 Mar 8;11:e75382. doi: 10.7554/eLife.75382. PMID: 35259092
Joly N, Beaumale E, Van Hove L, Martino L, Pintard L.
J Cell Biol. 2020 Jun 1;219(6):e201912037. doi: 10.1083/jcb.201912037.PMID: 32412594