Main research interest:

My main research interest is to understand how AAA+ ATPases are using ATP hydrolysis to remodel their specific substrates. Indeed, in all kingdom of life, a same structural unit (AAA+ core) is used for very versatile cellular activities (for example for transcription activation, protein degradation, or microtubule severing).

Understanding the conserved feature of such domain is critical to understand how these different molecular machines are working. In addition, more and more human diseases have been linked to defective proteins member of the AAA+ protein family. Strikingly, deep sequencing approaches have reported that the mutations responsible for the activity default were, in most of the time, located into the AAA+ core, pointing out the importance of understanding the molecular bases allowing the AAA+ core to use ATP hydrolysis and to transmit this "energy" to the remodeling motifs.

Currently, I am using the AAA+ microtubule-severing enzyme Katanin as system model.

Contact : email

Recent papers:

Enhanced Golden Gate Assembly: evaluating overhang strength for improved ligation efficiency

Patryk Strzelecki*, Nicolas Joly*, Pascal Hébraud, Elise Hoffmann, Grzegorz M Cech, Anna Kloska, Florent Busi, Wilfried Grange. (*co-first author)

Nucleic Acids Research, 2024;  https://doi-org.insb.bib.cnrs.fr/10.1093/nar/gkae809

Microtubule-binding domains in Katanin p80 subunit are essential for severing activity in C. elegans.

Beaumale E, Van Hove L, Pintard L, Joly N.

J Cell Biol. 2024 Apr 1;223(4):e202308023. doi: 10.1083/jcb.202308023. Epub 2024 Feb 8. PMID: 38329452

Cortical microtubule pulling forces contribute to the union of the parental genomes in the C. elegans zygote

Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Joly N, Pintard L.

Elife. 2022 Mar 8;11:e75382. doi: 10.7554/eLife.75382. PMID: 35259092

A survey of the kinome pharmacopeia reveals multiple scaffolds and targets for the development of novel anthelmintics.

Knox J, Joly N, Linossi EM, Carmona-Negrón JA, Jura N, Pintard L, Zuercher W, Roy PJ.

Sci Rep. 2021 Apr 28;11(1):9161. doi: 10.1038/s41598-021-88150-6. PMID: 33911106

Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry.

Tavernier N, Thomas Y, Vigneron S, Maisonneuve P, Orlicky S, Mader P, Regmi SG, Van Hove L, Levinson NM, Gasmi-Seabrook G, Joly N, Poteau M, Velez-Aguilera G, Gavet O, Castro A, Dasso M, Lorca T, Sicheri F, Pintard L. 

Nat Commun. 2021 Mar 26;12(1):1899. doi: 10.1038/s41467-021-21922-w. PMID: 33771996

PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly.

Velez-Aguilera G, Nkombo Nkoula S, Ossareh-Nazari B, Link J, Paouneskou D, Van Hove L, Joly N, Tavernier N, Verbavatz JM, Jantsch V, Pintard L.

Elife. 2020 Oct 8;9:e59510. doi: 10.7554/eLife.59510.

Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development.

Joly N, Beaumale E, Van Hove L, Martino L, Pintard L.

J Cell Biol. 2020 Jun 1;219(6):e201912037. doi: 10.1083/jcb.201912037.PMID: 32412594