Embedded Files
Natsumushi - Image analysis software for entomological studies -
Copyright(c) 2013-2025 Masahiko Tanahashi
Ver. 1.12 / Ver. 2.0β (download links will appear at the bottom of this page)
ntms1122.zip ..... Windows executable (14 Nov 2018; Ver. 1.12.2; FINAL VERSION of Natsumushi 1.x)
ntms20b_yyyymmdd.zip ..... Windows executable, recent beta version
ntms20b_yyyymmdd_nn.zip ..... Windows executable, recent beta version, minimum memory use & simple toolbar (for basic measurements, RECOMMENDED)
usermanual_line.pdf ..... Manual for basic line-length measurements (for Ver. 2.x)
sample_images.zip .... Sample images used in the original publication (Tanahashi & Fukatsu 2018)
Original publication (for version 1.x)
Tanahashi M., Fukatsu T. (2018) Natsumushi - An image measuring software for entomological studies. Entomological Science 21(3): 347-360
https://onlinelibrary.wiley.com/doi/full/10.1111/ens.12315 [PDF]
What are new features of Natsumushi 2.x ?
Quick, stable and saving memories
Being able to deal with TIFF and PNG (ver. 1.x can only open JPEG and BMP)
Powerful macro commands and fully customizable function keys
Curve length measurement by cubic spline approximation
3-D length measurement using two perpendicular photographs
Shape outline measurement by elliptic Fourier approximation
Shape matching analysis by genetic algorithm
Improved GabRat edge disruption analysis (GabRat-R; Tanahashi et al. 2025) [journal link]
Area Integration Index with Random Rearrangement (AIIRR; Tanahashi et al. 2025) [journal link] [website of AIIRR] [GitHub]
Related works using Natsumushi software
Tanahashi M, Lin M-Z, Lin C-P (2025) An overview of GabRat edge disruption and its new extensions for unbiased quantification of disruptive camouflaging patterns using randomization technique. PLOS ONE 20(7), e0300238. https://doi.org/10.1371/journal.pone.e0300238
Tanahashi M, Lin M-Z, Lin C-P (2025) Area Integration Index with Random Rearrangement (AIIRR): a new concept for quantifying disruptive colorations. Methods in Ecology and Evolution 16(8): 1781-1795. https://doi.org/10.1111/2041-210X.70085 (See also AIIR website)
Koga R, Moriyama M, Onodera-Tanifuji N, Ishii Y, Takai H, et al. (2022) Single mutation makes Escherichia coli an insect mutualist. Nature Microbiology 7: 1141-1150.
Anbutsu H, Moriyama M, Nikoh N, Hosokawa T, Futahashi R, Tanahashi M, Meng X-Y, Kuriwada T, Mori N, Oshima K, Hattori M, Fujie M, Satoh N, Maeda T, Shigenobu S, Koga R, Fukatsu T (2017) Small genome symbiont underlies cuticle hardness in beetles. Proceedings of the National Academy of Sciences of the United States of America 114(40): E8382-E8391.
Okude G, Futahashi R, Tanahashi M, Fukatsu T (2017) Laboratory rearing system for Ischnura senegalensis (Insecta: Odonata) enables detailed description of larval development and morphogenesis in dragonfly. Zoological Science 34(5): 386-397.
Download Links
Natsumushi 2.0 beta (Full version, 2025-04-07)
Natsumushi 2.0 beta (Minimum memory version, 2025-04-07)
Natsumushi 1.12.2 (Final version of Natsumushi 1.x) *Not recommended as it has some unfixed bugs
User Manual for line length measurements (pdf) (for Natsumushi 2.x)
User Manual for color measurements (mp4) (for Natsumushi 2.x)
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