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Intracapsular and intracellular algae. (A) Whole-mount image of egg capsule (Ec) showing clusters of algal cells. (B) TEM image of algal cells encased by an outer envelope (En; boxed region in 3A) on the inner border of the innermost egg capsule. (C and D) TEM images of intracapsular algae revealing starch granules (S), chloroplast grana (G), nuclei (N), and vacuoles (V). (E) Coronal vibratome section through the trunk region of a Stage-37 embryo. White spots are autofluorescing cells embedded in the somites (So). (F–J) Intracellular algae found within somites. (F) Algal cell embedded inside a salamander myocyte (M). (G) Higher magnification of intracellular alga in F. Salamander mitochondria (Mi) and myofibrils (My) occur in the surrounding host cytoplasm. (H) Paired algal cells within a host cell. (I) Algal cell surrounded by a thickened outer envelope. (J) Degraded algal cell without an outer envelope. F, flagella; Nt, neural tube; No, notochord; Yp, yolk platelet. (Scale bars: 100 μm in A; 2 μm in B–D; 1 mm in E; 2 μm in F–J.)
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